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[193818_s_at] neuroendocrine convertase 2 precursor like

Description F32A7.6 /REP_DB=WormBase Gene ID /WP=CE09847 /GEN=aex-5 /TR=P91863 /GB=CAB05501.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=neuroendocrine convertase 2 precursor like
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 71      6grp_total > 41      4grp_total > 47      6grp_cgc > 60      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 485
1 2 3 4 range
A 599.5
(3)
P 		200.2
(-3)
P 		225.6
(-2.6)
P 		273.7
(-1.9)
P 		399
B 685.1
(3)
P 		228.1
(-3)
P 		259.6
(-2.6)
P 		251.7
(-2.7)
P 		457
C 645
(3)
P 		211.4
(-3)
P 		286.8
(-2)
P 		227.6
(-2.8)
P 		434
avr 643.2
(3) 		213.2
(-3) 		257.3
(-2.4) 		251
(-2.5) 		430

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 209
1 2 3 4 5 6 range
A 380.2
(0.3)
P 		444.2
(2.8)
P 		413.1
(1.6)
P 		449.9
(3)
P 		309.4
(-2.5)
P 		295.9
(-3)
P 		154
B 405.3
(2.8)
P 		290.5
(-1.5)
P 		410.1
(3)
P 		325.9
(-0.1)
P 		375.4
(1.7)
P 		248.9
(-3)
P 		161
C 373.2
(0.2)
P 		362
(-0.1)
P 		458
(3)
P 		386
(0.6)
P 		274.2
(-3)
P 		277.7
(-2.9)
P 		184
avr 386.2
(1.4) 		365.6
(0.6) 		427.1
(3) 		387.3
(1.4) 		319.7
(-1.2) 		274.2
(-3) 		153
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 218.3
(3)
P 		43.5
(-2.9)
P 		59
(-2.4)
P 		40.1
(-3)
P 		178
B 218.5
(3)
P 		56.3
(-2.9)
P 		60.1
(-2.8)
P 		53.6
(-3)
P 		165
C 213.5
(3)
P 		60.8
(-2.2)
P 		100
(-0.9)
P 		38.4
(-3)
P 		175
avr 216.8
(3) 		53.5
(-2.7) 		73
(-2) 		44
(-3) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 70.1
(-3)
P 		158
(3)
P 		90
(-1.6)
P 		114.1
(0)
P 		93.4
(-1.4)
P 		72
(-2.9)
P 		88
B 141
(2)
P 		96.7
(-2.1)
P 		99.6
(-1.8)
P 		86.3
(-3)
P 		152.5
(3)
P 		92.8
(-2.4)
P 		66
C 138.3
(3)
P 		110.5
(0.3)
P 		116.6
(0.9)
P 		77.6
(-3)
P 		88.7
(-1.9)
P 		93
(-1.5)
P 		61
avr 116.5
(2.1) 		121.7
(3) 		102.1
(-0.3) 		92.7
(-1.9) 		111.5
(1.3) 		85.9
(-3) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193818_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1622
Sequence Type Exemplar sequence
Representative Public ID F32A7.6 NCBI
Target Description F32A7.6 /REP_DB=WormBase Gene ID /WP=CE09847 /GEN=aex-5 /TR=P91863 /GB=CAB05501.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=neuroendocrine convertase 2 precursor like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061193(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061193 NCBI Caenorhabditis elegans Aboc, EXpulsion defective family member (aex-5) (aex-5) mRNA, complete cds. 11/11 None
SNAP00000011758 ENSEMBL cdna:SNAP chromosome:CEL140:I:14858258:14863849:-1 11/11 None
GENEFINDER00000011762 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14858258:14863521:-1 11/11 None
F32A7.6 ENSEMBL cdna:known chromosome:CEL140:I:14858188:14863908:-1 gene:F32A7.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14858188-14863780(-) 90.91 100.0
Public Domain and Genome References
Gene Title neuroendocrine convertase 2 precursor like
Gene Symbol aex-5
Ensembl F32A7.6
Entrez Gene 173358 Entrez gene
SwissProt P91863 EMBL-EBI
WormBase CE28554 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
45045 secretory pathway inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4289 subtilase activity inferred from electronic annotation QuickGO AmiGO
16808 proprotein convertase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05501 Hypothetical protein F32A7.6 [Caenorhabditis elegans] ref|NP_493594.1| Aboc, EXpulsion defective family member (aex-5) [Caenorhabditis elegans] 0.0
blast CAE72534 Hypothetical protein CBG19714 [Caenorhabditis briggsae] 0.0
blast XP_699895 PREDICTED: similar to proprotein convertase 6B [Danio rerio] 7.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR002884 EMBL-EBI Peptidase S8B, proprotein convertase, P domain 8.1E-45
Pfam IPR000209 EMBL-EBI Peptidase S8 and S53, subtilisin, kexin, sedolisin 1.5E-50
Pfam IPR002884 EMBL-EBI Peptidase S8B, proprotein convertase, P domain 8.1E-45
Pfam IPR000209 EMBL-EBI Peptidase S8 and S53, subtilisin, kexin, sedolisin 1.4E-48
Sequence
Target Sequence 
>C. ELEGANS:193818_S_AT
aacgtgacattcaacacctcattgctcgcacctccaatgcaagtgcaatccgtgacgtgg
aattgtatgaaaattctgctggcctaaatttccacccaaaagttggattcggtctactga
atgcacagaaactcgtggtaatggctgccacgtgggagaatgtggcacctcaagtgactt
gcgagaaaatgaatctagcgaatggaataatcgataattcggattgtgacgtcacaaaag
tagaacgtgtaattgtctccggatctatcatccatccacatcgaggacaagttcaaatca
gattggaatcgccacggggcacaatctccgaactgcttccacttcgtcccaaagacacat
cgcgagatcttctcgactggaactttgtatccgtcaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGTGACATTCAACACCTCATTGC 21 145 1316 Antisense
TGCTCGCACCTCCAATGCAAGTGCA 542 581 1338 Antisense
AATGCAAGTGCAATCCGTGACGTGG 506 165 1351 Antisense
AAATTCTGCTGGCCTAAATTTCCAC 466 111 1386 Antisense
GAATGTGGCACCTCAAGTGACTTGC 220 333 1473 Antisense
GAACGTGTAATTGTCTCCGGATCTA 567 347 1558 Antisense
ATCTATCATCCATCCACATCGAGGA 577 31 1578 Antisense
TTGGAATCGCCACGGGGCACAATCT 470 707 1618 Antisense
CCACTTCGTCCCAAAGACACATCGC 466 275 1654 Antisense
GACACATCGCGAGATCTTCTCGACT 521 365 1669 Antisense
GACTGGAACTTTGTATCCGTCAACT 167 377 1690 Antisense
Sequence Source Affymetrix Proprietary Database