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[193807_at] 3-beta hydroxysteriod dehydrogenase/isomerase family

Description Y6B3B.11 /REP_DB=WormBase Gene ID /WP=CE20185 /TR=Q9XWF0 /GB=CAA21722.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=3-beta hydroxysteriod dehydrogenase-isomerase family
Cluster 6grp_total > 18      4grp_total > 31      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 4.5
(-3)
A 		7.8
(-0.8)
A 		5.3
(-2.5)
A 		13.5
(3)
A 		9
B 28.8
(3)
A 		13.5
(-1.3)
A 		7.3
(-3)
A 		26.5
(2.4)
A 		22
C 41.9
(3)
A 		10.4
(-2.9)
A 		20.4
(-1)
A 		9.6
(-3)
A 		32
avr 25.1
(3) 		10.6
(-3) 		11
(-2.8) 		16.5
(-0.6) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 83.2
(3)
A 		10.5
(-2.8)
A 		39
(-0.5)
A 		7.8
(-3)
A 		8.9
(-2.9)
A 		23.2
(-1.8)
A 		75
B 6.6
(-2.9)
A 		8.4
(-2.4)
A 		6.9
(-2.8)
A 		27.4
(3)
A 		6.2
(-3)
A 		7.1
(-2.7)
A 		21
C 27.9
(1.4)
A 		19.7
(-0.3)
A 		14
(-1.5)
A 		6.6
(-3)
A 		35.6
(3)
A 		14.3
(-1.4)
A 		29
avr 39.2
(3) 		12.9
(-3) 		20
(-1.4) 		13.9
(-2.8) 		16.9
(-2.1) 		14.9
(-2.5) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 1.7
(0)
A 		1.7
(0)
A 		1.4
(-3)
A 		2
(3)
A 		1
B 9.2
(3)
A 		3.3
(-1.7)
A 		1.7
(-3)
A 		5.7
(0.2)
A 		8
C 13.9
(3)
A 		3
(-2.3)
A 		7.1
(-0.3)
A 		1.6
(-3)
A 		12
avr 8.3
(3) 		2.7
(-3) 		3.4
(-2.3) 		3.1
(-2.6) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 15.3
(3)
A 		3.7
(-2.2)
A 		8.5
(-0.1)
A 		2
(-3)
A 		2.7
(-2.7)
A 		5.7
(-1.3)
A 		13
B 2.3
(-2.4)
A 		2.8
(-1.8)
A 		1.7
(-3)
A 		7.3
(3)
A 		2.5
(-2.1)
A 		2.6
(-2)
A 		6
C 10.4
(2.4)
A 		6
(-0.2)
A 		3.6
(-1.6)
A 		1.3
(-3)
A 		11.5
(3)
A 		4.8
(-0.9)
A 		10
avr 9.3
(3) 		4.2
(-2.3) 		4.6
(-1.9) 		3.5
(-3) 		5.6
(-0.8) 		4.4
(-2.1) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193807_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.978
Sequence Type Exemplar sequence
Representative Public ID Y6B3B.11 NCBI
Target Description Y6B3B.11 /REP_DB=WormBase Gene ID /WP=CE20185 /TR=Q9XWF0 /GB=CAA21722.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=3-beta hydroxysteriod dehydrogenase-isomerase family
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061001(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061001 NCBI Caenorhabditis elegans Y6B3B.11 (Y6B3B.11) mRNA, complete cds. 11/11 None
SNAP00000043747 ENSEMBL cdna:SNAP chromosome:CEL140:I:13728243:13732571:-1 11/11 None
GENEFINDER00000043760 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13728243:13732571:-1 11/11 None
Y6B3B.11 ENSEMBL cdna:known chromosome:CEL140:I:13728243:13732571:-1 gene:Y6B3B.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13728173-13732502(-) 100.0 100.0
Public Domain and Genome References
Gene Title 3-beta hydroxysteriod dehydrogenase/isomerase family
Ensembl Y6B3B.11
Entrez Gene 189371 Entrez gene
SwissProt Q9XWF0 EMBL-EBI
WormBase CE20185 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6694 steroid biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3854 3-beta-hydroxy-delta5-steroid dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21722 Hypothetical protein Y6B3B.11 [Caenorhabditis elegans] ref|NP_493402.1| Y6B3B.11 [Caenorhabditis elegans] 0.0
blast CAE69596 Hypothetical protein CBG15823 [Caenorhabditis briggsae] 1.0E-103
blast AAB52622 Hypothetical protein ZC8.1 [Caenorhabditis elegans] ref|NP_508851.1| ZC8.1 [Caenorhabditis elegans] 1.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR002225 EMBL-EBI 3-beta hydroxysteroid dehydrogenase/isomerase 6.1E-24
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493402.1 2 332-354,430-452
Y6B3B.11 2 332-354,430-452
SNAP00000043747 2 332-354,430-452
GENEFINDER00000043760 2 332-354,430-452
Sequence
Target Sequence 
>C. ELEGANS:193807_AT
tgtggattgccacctgtaagcctaatggcgtagaagcgcagactcagctgtcctcggtgg
ccaactgtatccagggacttgtcaaggcagagttggcgttgagatggtcagacaccccac
atggacagatctacaacataatggacaagacccccgttggcactttctctttctggaccc
ctctaaacattgctctggggttctccagctcgatgatcacagttccagcgacccctattc
gattgttcgcctatttatctcaaatcatcgcggatcgaatgcgtattgacccgattgtta
gtgtgttggaagttgatttgcttcttgttaacaatacatttaacattgagaaggccgagc
gggacctgggatatgaaccctcggtttcagcaattccagagatcatcgagcactacctcc
accgtcttcctccagacgtcgtccgtcccaaaggccgctctgacttctatgtcaaagtcg
ccgtacttgtattggggaccattttgatcttcgtcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGGATTGCCACCTGTAAGCCTAA 193 555 818 Antisense
TAGAAGCGCAGACTCAGCTGTCCTC 82 653 848 Antisense
CTCGGTGGCCAACTGTATCCAGGGA 155 229 870 Antisense
GCGTTGAGATGGTCAGACACCCCAC 571 291 913 Antisense
GACACCCCACATGGACAGATCTACA 585 367 928 Antisense
CTAAACATTGCTCTGGGGTTCTCCA 701 225 1000 Antisense
TCTCCAGCTCGATGATCACAGTTCC 86 611 1019 Antisense
CAGCGACCCCTATTCGATTGTTCGC 497 199 1043 Antisense
GGGACCTGGGATATGAACCCTCGGT 263 303 1178 Antisense
GTCGCCGTACTTGTATTGGGGACCA 14 471 1294 Antisense
TGGGGACCATTTTGATCTTCGTCGC 546 553 1310 Antisense
Sequence Source Affymetrix Proprietary Database