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[193762_at]

Description F32A11.5 /REP_DB=WormBase Gene ID /WP=CE18656 /TR=Q9XV47 /GB=CAB04228.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=DNA topoisomerase II (N-terminal region) (2 domains)
Cluster 6grp_total > 06      4grp_total > 40      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 6.6
(1.8)
A 		7.4
(3)
A 		3.4
(-3)
A 		6.3
(1.4)
A 		4
B 5.6
(-3)
A 		5.9
(-2.9)
A 		36.3
(3)
A 		27.8
(1.3)
A 		31
C 24.2
(3)
A 		22.4
(2.3)
A 		23.2
(2.6)
A 		7.7
(-3)
A 		17
avr 12.1
(-2.9) 		11.9
(-3) 		21
(3) 		13.9
(-1.7) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 8.5
(3)
A 		5.5
(0.4)
A 		1.6
(-3)
A 		3.2
(-1.6)
A 		5.2
(0.1)
A 		4.6
(-0.4)
A 		7
B 24.3
(3)
A 		23.1
(2.7)
A 		19
(1.6)
A 		5
(-2.1)
A 		2.7
(-2.8)
A 		1.8
(-3)
A 		23
C 19.7
(3)
A 		4
(-2.1)
A 		1.4
(-3)
A 		1.9
(-2.8)
A 		4
(-2.1)
A 		3.4
(-2.3)
A 		18
avr 17.5
(3) 		10.9
(0.2) 		7.3
(-1.3) 		3.4
(-3) 		4
(-2.7) 		3.3
(-3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 2.4
(3)
A 		1.6
(-0.2)
A 		0.9
(-3)
A 		0.9
(-3)
A 		2
B 1.8
(-2.7)
A 		1.5
(-3)
A 		8.4
(3)
A 		5.9
(0.8)
A 		7
C 8
(2.9)
A 		6.4
(1.5)
A 		8.1
(3)
A 		1.3
(-3)
A 		7
avr 4.1
(-0.3) 		3.2
(-2) 		5.8
(3) 		2.7
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1.6
(1.8)
A 		1.9
(3)
A 		0.4
(-3)
A 		0.8
(-1.4)
A 		1.6
(1.8)
A 		1.1
(-0.2)
A 		2
B 8.5
(3)
A 		7.7
(2.4)
A 		4.6
(-0)
A 		1.3
(-2.5)
A 		1.1
(-2.7)
A 		0.7
(-3)
A 		8
C 7.3
(3)
A 		1.2
(-2.2)
A 		0.3
(-3)
A 		0.4
(-2.9)
A 		1.3
(-2.1)
A 		1.1
(-2.3)
A 		7
avr 5.8
(3) 		3.6
(0.4) 		1.8
(-1.8) 		0.8
(-3) 		1.3
(-2.4) 		1
(-2.8) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193762_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3409
Sequence Type Exemplar sequence
Representative Public ID F32A11.5 NCBI
Target Description F32A11.5 /REP_DB=WormBase Gene ID /WP=CE18656 /TR=Q9XV47 /GB=CAB04228.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=DNA topoisomerase II (N-terminal region) (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description Only library file information is available for this probe set. See probe design information above.
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000006592 ENSEMBL cdna:SNAP chromosome:CEL140:II:13130905:13131804:1 11/11 None
F32A11.5 ENSEMBL cdna:known chromosome:CEL140:II:13130905:13131804:1 gene:F32A11.5 11/11 None
F32A11.5.pseudo ENSEMBL cdna:pseudogene chromosome:CEL140:II:13130905:13134108:1 gene:F32A11.5.pseudo 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000006604 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13130901-13131801(+) 100.0 100.0
Public Domain and Genome References
Ensembl F32A11.5
F32A11.5.pseudo
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T21619 hypothetical protein F32A11.5 - Caenorhabditis elegans 1.0E-151
blast CAA94177 Hypothetical protein K12D12.1 [Caenorhabditis elegans] emb|CAA88867.1| Hypothetical protein K12D12.1 [Caenorhabditis elegans] ref|NP_496536.1| K12D12.1 [Caenorhabditis elegans] sp|Q23670|TOP2_CAEEL Probable DNA topoisomerase 2 (DNA topoisomerase II) 1.0E-111
blast CAB04227 Hypothetical protein F32A11.4 [Caenorhabditis elegans] ref|NP_496791.1| F32A11.4 [Caenorhabditis elegans] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 4.5E-11
Sequence
Target Sequence 
>C. ELEGANS:193762_AT
agaagaaatcccacctggagcacatcctcctgcgtcccgacagctacatcggctcaatcg
agcacaatgagaagaagccgatgtgggtctacaacatgaaggacagcaagctggaacaga
gagatcctaagatgaacacaatcaagatcaccatcaatacggagaaaaatgagatctctg
tctacaacaacggcaaaggaatcccagtcacccagcacaaagtggagaaagtctacgtgc
cagagctgatttttggagctcttctaacatcttacaactacaatgacgacgagaagaagc
tgacaggaggaagaaacggatatggagcaaagctttgcaaccttttctccacaaaggtca
ctctggagacgtcttcagtggattacaaaagtgcattcaagcaaacgtggatcaacaaca
tgacgcgtgatgaggagccgacgattgttccgagcaccgacgaagacttcacgaagatca
cattctcgccggatctggcaaagttcaagacgaaggagctggacgatgacatctgccatt
tgatggctcggcgtgcatatgatgttgctggatcctgtaaaggagttgccgtgtcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGAAATCCCACCTGGAGCACAT 74 75 179 Antisense
GCAAGCTGGAACAGAGAGATCCTAA 702 319 284 Antisense
TTTTGGAGCTCTTCTAACATCTTAC 329 679 429 Antisense
GCTCTTCTAACATCTTACAACTACA 277 301 436 Antisense
GAAGCTGACAGGAGGAAGAAACGGA 456 343 474 Antisense
GCTTTGCAACCTTTTCTCCACAAAG 129 305 510 Antisense
CTTTTCTCCACAAAGGTCACTCTGG 353 223 520 Antisense
GCTCGGCGTGCATATGATGTTGCTG 553 299 724 Antisense
GCATATGATGTTGCTGGATCCTGTA 340 309 733 Antisense
TGATGTTGCTGGATCCTGTAAAGGA 51 565 738 Antisense
ATCCTGTAAAGGAGTTGCCGTGTCC 21 13 750 Antisense
Sequence Source Affymetrix Proprietary Database