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[193759_at]

Description B0304.1 /REP_DB=WormBase Gene ID /WP=CE02423 /GEN=hlh-1 /TR=SW:P22980 /GB=AAK31389.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=helix-loop-helix transcription factor
Cluster 6grp_high1_10_10 > 27      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_total > 27      4grp_total > 21      6grp_cgc > 06      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 103.2
(0.9)
P 		100.6
(0.5)
P 		72.2
(-3)
P 		120.2
(3)
P 		48
B 122.5
(2.2)
P 		93.2
(-1.8)
P 		84.8
(-3)
P 		127.9
(3)
P 		43
C 107.8
(0.8)
P 		92.8
(-0.4)
P 		62.8
(-3)
P 		133
(3)
P 		70
avr 111.2
(1.2) 		95.5
(-0.5) 		73.3
(-3) 		127
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 125.3
(3)
P 		68
(-0.6)
P 		76.8
(-0.1)
P 		30
(-3)
A 		63.5
(-0.9)
P 		66
(-0.7)
P 		95
B 106.7
(3)
P 		90.5
(1.4)
P 		76.4
(-0)
P 		65.6
(-1.1)
P 		46.3
(-3)
P 		58.5
(-1.8)
P 		60
C 103.6
(3)
P 		70.8
(-0.1)
P 		43.1
(-2.6)
P 		44.7
(-2.5)
A 		39.3
(-3)
P 		78.9
(0.7)
P 		64
avr 111.9
(3) 		76.4
(-0.3) 		65.4
(-1.3) 		46.8
(-3) 		49.7
(-2.7) 		67.8
(-1.1) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 37.6
(3)
P 		21.8
(-1.7)
P 		18.9
(-2.6)
P 		17.6
(-3)
P 		20
B 39.1
(3)
P 		23
(-2)
P 		19.7
(-3)
P 		27.3
(-0.6)
P 		19
C 35.7
(3)
P 		26.7
(-0.9)
P 		21.9
(-3)
P 		22.4
(-2.8)
P 		14
avr 37.5
(3) 		23.8
(-1.8) 		20.2
(-3) 		22.4
(-2.2) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 23.1
(2.6)
P 		24.2
(3)
P 		16.7
(0.3)
P 		7.6
(-3)
A 		19.2
(1.2)
P 		16.1
(0.1)
P 		17
B 37.1
(3)
P 		30.1
(0.9)
P 		18.5
(-2.7)
P 		17.4
(-3)
P 		18.8
(-2.6)
P 		21.8
(-1.7)
P 		20
C 38.4
(3)
P 		21.6
(-0.4)
P 		11
(-2.6)
P 		9
(-3)
A 		12.7
(-2.2)
P 		26.4
(0.6)
P 		29
avr 32.9
(3) 		25.3
(0.9) 		15.4
(-1.9) 		11.3
(-3) 		16.9
(-1.4) 		21.4
(-0.2) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193759_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5377
Sequence Type Exemplar sequence
Representative Public ID B0304.1 NCBI
Target Description B0304.1 /REP_DB=WormBase Gene ID /WP=CE02423 /GEN=hlh-1 /TR=SW:P22980 /GB=AAK31389.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=helix-loop-helix transcription factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026722(11), NM_001026721(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026722 NCBI Caenorhabditis elegans Helix Loop Helix family member (hlh-1) (hlh-1) mRNA, complete cds. 11/11 None
NM_001026721 NCBI Caenorhabditis elegans Helix Loop Helix family member (hlh-1) (hlh-1) mRNA, complete cds. 11/11 None
B0304.1c ENSEMBL cdna:known chromosome:CEL140:II:4519056:4522444:1 gene:B0304.1 11/11 None
B0304.1b ENSEMBL cdna:known chromosome:CEL140:II:4519056:4522424:1 gene:B0304.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062397 8/11 Cross Hyb Matching Probes None
SNAP00000018303 7/11 Cross Hyb Matching Probes None
GENEFINDER00000018315 8/11 Cross Hyb Matching Probes None
B0304.1a 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173788 3 2 NM_001026721 193759_at (11)
NM_001026722 193759_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4519054-4522423(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0304.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31389 Helix loop helix protein 1, isoform c [Caenorhabditis elegans] ref|NP_001021893.1| Helix Loop Helix family member (hlh-1) [Caenorhabditis elegans] 1.0E-152
blast AAN39684 Helix loop helix protein 1, isoform b [Caenorhabditis elegans] ref|NP_001021892.1| Helix Loop Helix family member (hlh-1) [Caenorhabditis elegans] sp|P22980|MYOD1_CAEEL Myoblast determination protein 1 homolog (MyoD protein 1) (Helix-loop-helix protein 1) gb|AAA16289.1| CeMyoD 1.0E-145
blast AAN39683 Helix loop helix protein 1, isoform a [Caenorhabditis elegans] ref|NP_494798.4| Helix Loop Helix family member (hlh-1) [Caenorhabditis elegans] gb|AAA16290.1| CeMyoD, alternatively spliced product 1.0E-140
blast B36289 CeMyoD protein - Caenorhabditis elegans 1.0E-140
blast AAN39684 Helix loop helix protein 1, isoform b [Caenorhabditis elegans] ref|NP_001021892.1| Helix Loop Helix family member (hlh-1) [Caenorhabditis elegans] sp|P22980|MYOD1_CAEEL Myoblast determination protein 1 homolog (MyoD protein 1) (Helix-loop-helix protein 1) gb|AAA16289.1| CeMyoD 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 2.0E-16
Sequence
Target Sequence 
>C. ELEGANS:193759_AT
cagagccgagtcgacagtcaacatgaggacactacaacgtctactgctggcggagccggt
gtcggtggaccgcggagaaccaaattcgttctttcagtggacagacggaaggcggcaaca
atgcgtgagagacggcgtcttcggaaagtcaacgaagcttttgaagttgtgaagcaaaga
acgtgtccgaatccgaatcaacgtcttcctaaagtcgagattctcagatcagcaattgat
tatatcaataatctagaaagaatgctgcaacaagccggaaaaatgacaaagattatggag
cagaaccagcatttgcagatgacacagcagattaatggagctccacctcacgattatgtg
acttcctctcactttgcatcgagctcttacaatccagaaaatatgtttgacgatgatgat
ttgacggattcggacgacgatagagatcatcataagctcgggaacgcggtcgatct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGAGCCGAGTCGACAGTCAACATG 638 203 376 Antisense
GGACACTACAACGTCTACTGCTGGC 57 527 402 Antisense
GGTGGACCGCGGAGAACCAAATTCG 656 455 439 Antisense
AAATTCGTTCTTTCAGTGGACAGAC 306 115 457 Antisense
GTGGACAGACGGAAGGCGGCAACAA 105 491 472 Antisense
GAGACGGCGTCTTCGGAAAGTCAAC 117 391 504 Antisense
GCAAAGAACGTGTCCGAATCCGAAT 359 317 549 Antisense
GAATCCGAATCAACGTCTTCCTAAA 443 329 564 Antisense
GTCTTCCTAAAGTCGAGATTCTCAG 282 469 578 Antisense
GGAGCAGAACCAGCATTTGCAGATG 222 521 672 Antisense
ATAAGCTCGGGAACGCGGTCGATCT 389 25 827 Antisense
Sequence Source Affymetrix Proprietary Database