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[193752_s_at] mitochondrial glycerol-3-phosphate dehydrogenase

Description T25G3.4 /REP_DB=WormBase Gene ID /WP=CE14180 /TR=SW:P90795 /GB=CAA96690.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=mitochondrial glycerol-3-phosphate dehydrogenase
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 74      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 55      6grp_total > 75      4grp_total > 39      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 339
1 2 3 4 range
A 1465.1
(3)
P 		1172.7
(-2.5)
P 		1148.2
(-3)
P 		1195.5
(-2.1)
P 		317
B 1487.1
(3)
P 		1247.5
(-1.9)
P 		1192.6
(-3)
P 		1451.8
(2.3)
P 		295
C 1446.5
(3)
P 		1196.5
(-2.5)
P 		1213.2
(-2.1)
P 		1174.1
(-3)
P 		272
avr 1466.2
(3) 		1205.6
(-2.6) 		1184.7
(-3) 		1273.8
(-1.1) 		282

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 934
1 2 3 4 5 6 range
A 1216.9
(-3)
P 		1726.8
(0.3)
P 		1713.2
(0.2)
P 		2130.5
(2.9)
P 		1414.5
(-1.7)
P 		2151.2
(3)
P 		934
B 1473.1
(-1.5)
P 		1475.9
(-1.4)
P 		1366.2
(-3)
P 		1761.7
(2.7)
P 		1395.9
(-2.6)
P 		1780.5
(3)
P 		414
C 1491.4
(-2.3)
P 		1416.3
(-3)
P 		2028.2
(2.7)
P 		2063.3
(3)
P 		1502.3
(-2.2)
P 		1877.6
(1.3)
P 		647
avr 1393.8
(-3) 		1539.7
(-1.5) 		1702.5
(0.1) 		1985.2
(3) 		1437.6
(-2.6) 		1936.4
(2.5) 		591
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 358
1 2 3 4 range
A 533.4
(3)
P 		254.6
(-1.7)
P 		300.2
(-0.9)
P 		175
(-3)
P 		358
B 474.2
(3)
P 		308.1
(-2)
P 		276.4
(-3)
P 		309.4
(-2)
P 		198
C 478.8
(3)
P 		344
(0.1)
P 		422.8
(1.8)
P 		198
(-3)
P 		281
avr 495.5
(3) 		302.2
(-1.3) 		333.1
(-0.6) 		227.5
(-3) 		268

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 440
1 2 3 4 5 6 range
A 224.3
(-3)
P 		614.1
(3)
P 		373.1
(-0.7)
P 		540.3
(1.9)
P 		426.8
(0.1)
P 		523.8
(1.6)
P 		390
B 512.6
(0.3)
P 		491.3
(-0.1)
P 		331.7
(-3)
P 		466.6
(-0.6)
P 		567.1
(1.3)
P 		663.9
(3)
P 		332
C 552.8
(0.9)
P 		432.2
(-2.5)
P 		516.2
(-0.2)
P 		414.9
(-3)
P 		486.1
(-1)
P 		628.5
(3)
P 		214
avr 429.9
(-2.3) 		512.5
(0.2) 		407
(-3) 		473.9
(-1) 		493.3
(-0.4) 		605.4
(3) 		198
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193752_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1987
Sequence Type Exemplar sequence
Representative Public ID T25G3.4 NCBI
Target Description T25G3.4 /REP_DB=WormBase Gene ID /WP=CE14180 /TR=SW:P90795 /GB=CAA96690.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=mitochondrial glycerol-3-phosphate dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059714(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059714 NCBI Caenorhabditis elegans T25G3.4 (T25G3.4) mRNA, complete cds. 11/11 None
SNAP00000031269 ENSEMBL cdna:SNAP chromosome:CEL140:I:7573211:7575511:1 11/11 None
GENEFINDER00000031283 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7573211:7575511:1 11/11 None
T25G3.4 ENSEMBL cdna:known chromosome:CEL140:I:7572711:7575850:1 gene:T25G3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7572643-7575442(+) 100.0 100.0
Public Domain and Genome References
Gene Title mitochondrial glycerol-3-phosphate dehydrogenase
Ensembl T25G3.4
Entrez Gene 172509 Entrez gene
SwissProt P90795 EMBL-EBI
WormBase CE14180 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:259146_AT glycerol-3-phosphate dehydrogenase, putative at
CANINE:1605349_AT similar to Glycerol-3-phosphate dehydrogenase, mitochondrial precursor (GPD-M) (GPDH-M) (mtGPD) cfa
CANINE:1601887_AT similar to Glycerol-3-phosphate dehydrogenase, mitochondrial precursor (GPD-M) (GPDH-M) (mtGPD) cfa
CANINE_2:CFAAFFX.14561.1.S1_AT similar to Glycerol-3-phosphate dehydrogenase, mitochondrial precursor (GPD-M) (GPDH-M) (mtGPD) cfa
DROSOPHILA_2:1631474_S_AT dm
DROSGENOME1:152312_AT dm
CHICKEN:GGA.13576.1.S1_AT similar to glycerol-3-phosphate dehydrogenase 2; glycerol phosphate dehydrogenase 1, mitochondrial; FAD-linked glycerol-3-phosphate dehydrogenase gga
CHICKEN:GGAAFFX.23939.1.S1_S_AT similar to glycerol-3-phosphate dehydrogenase 2; glycerol phosphate dehydrogenase 1, mitochondrial; FAD-linked glycerol-3-phosphate dehydrogenase gga
HG-U133B:225447_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133_PLUS_2:225447_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133_PLUS_2:211613_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133A:211613_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133A_2:211613_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U95AV2:41021_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U95AV2:41022_R_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133_PLUS_2:210007_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133A_2:210007_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133A:210007_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-FOCUS:210007_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HU35KSUBA:U12424_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HUGENEFL:U12424_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HU35KSUBC:RC_AA465701_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
U133_X3P:G1806627_3P_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
U133_X3P:G1020314_3P_A_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
U133_X3P:HS.108646.0.S1_3P_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U95C:58522_S_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U95E:68626_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U95D:83099_AT glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U95C:61118_AT Glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133_PLUS_2:243598_AT Glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U133B:243598_AT Glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U95B:42720_AT Glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HG-U95D:70059_AT Glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
HU35KSUBD:RC_AA453987_AT Glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
U133_X3P:HS.144802.0.A1_3P_AT Glycerol-3-phosphate dehydrogenase 2 (mitochondrial) hs
MG-U74BV2:114138_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MU11KSUBA:D50430_S_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MG-U74AV2:98984_F_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MG-U74BV2:113012_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MG-U74BV2:117316_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MG-U74BV2:114139_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MOE430A:1417434_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MOUSE430A_2:1417434_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MOUSE430_2:1417434_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MOE430B:1428323_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MOUSE430_2:1428323_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MOE430B:1452741_S_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MOUSE430_2:1452741_S_AT glycerol phosphate dehydrogenase 2, mitochondrial mm
MOUSE430_2:1459689_AT TISP38 mm
MOE430B:1459689_AT TISP38 mm
MG-U74AV2:162210_R_AT TISP38 mm
RG-U34A:X78593_G_AT glycerol-3-phosphate dehydrogenase 2 rn
RG-U34A:X78593_AT glycerol-3-phosphate dehydrogenase 2 rn
RAE230A:1387670_AT glycerol-3-phosphate dehydrogenase 2 rn
RAT230_2:1387670_AT glycerol-3-phosphate dehydrogenase 2 rn
RG-U34A:U83880UTR#1_G_AT Glycerol-3-phosphate dehydrogenase 2 rn
RG-U34A:U83880UTR#1_AT Glycerol-3-phosphate dehydrogenase 2 rn
RG-U34A:U83879_AT Glycerol-3-phosphate dehydrogenase 2 rn
RAE230A:1369666_AT Glycerol-3-phosphate dehydrogenase 2 rn
RAT230_2:1369666_AT Glycerol-3-phosphate dehydrogenase 2 rn
RG-U34B:RC_AI044925_AT Glycerol-3-phosphate dehydrogenase 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6072 glycerol-3-phosphate metabolism inferred from electronic annotation QuickGO AmiGO
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
9331 glycerol-3-phosphate dehydrogenase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4368 glycerol-3-phosphate dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA96690 Hypothetical protein T25G3.4 [Caenorhabditis elegans] emb|CAA98123.1| Hypothetical protein T25G3.4 [Caenorhabditis elegans] ref|NP_492115.1| T25G3.4 [Caenorhabditis elegans] sp|P90795|GPDM_CAEEL Probable glycerol-3-phosphate dehydrogenase, mitochondrial precursor (GPD-M) (GPDH-M) 0.0
blast CAE66579 Hypothetical protein CBG11896 [Caenorhabditis briggsae] 0.0
blast AAH73694 MGC83596 protein [Xenopus laevis] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.4E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.3E-4
Pfam IPR006076 EMBL-EBI FAD dependent oxidoreductase 4.4E-118
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.4E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.3E-4
Pfam IPR006076 EMBL-EBI FAD dependent oxidoreductase 1.5E-85
Sequence
Target Sequence 
>C. ELEGANS:193752_S_AT
gccaattgttgggcaacgccttcatcccgaattcccatatttagatgctgaggttcgtta
cgctgttcgtgaatatgcctgcacagcaatcgatgttatcgccagaagaatgcgtcttgc
tttcttgaacacatatgccgctcatgaagtacttcctgatgttgttcgtgttatgggaca
agaacttggatggtcatcagctgaacaacgagctcaattggaaaaggcacgtacttttat
tgatatggaaatgggacagaatgctaaacaaactgcagtatccaatgttgcactgaattt
gacaaaagaagaaatgcaaagagccaaggaacgattccaacaattggataaagatcgcaa
aggacatatcacagtcaacgatcttcgcaagcatttcagagaacataaccaaaagattga
tgaacgtgttctccacgaacttctaaacgaagtggatctcaataagaacggagaaattga
aatcgccgaattcttccagctgtactctggattgaaaggaggtcaactcaccggaaatcg
actcgtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCAATTGTTGGGCAACGCCTTCAT 354 277 1575 Antisense
TTCATCCCGAATTCCCATATTTAGA 280 687 1595 Antisense
GATGCTGAGGTTCGTTACGCTGTTC 691 413 1618 Antisense
TACGCTGTTCGTGAATATGCCTGCA 437 649 1633 Antisense
GCCTGCACAGCAATCGATGTTATCG 161 281 1651 Antisense
ATGCGTCTTGCTTTCTTGAACACAT 693 41 1684 Antisense
ATATGCCGCTCATGAAGTACTTCCT 381 21 1707 Antisense
GGACATATCACAGTCAACGATCTTC 391 525 1936 Antisense
AACGATCTTCGCAAGCATTTCAGAG 3 145 1951 Antisense
GAACGTGTTCTCCACGAACTTCTAA 667 347 1996 Antisense
CAACTCACCGGAAATCGACTCGTCG 173 189 2098 Antisense
Sequence Source Affymetrix Proprietary Database