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[193748_at] sodium/potassium-transporting ATPase alpha-2 chain

Description C02E7.1 /REP_DB=WormBase Gene ID /WP=CE07838 /TR=O16436 /GB=AAC24161.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=sodium-potassium-transporting ATPase alpha-2 chain
Cluster 6grp_total > 69      4grp_total > 24      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 4.8
(-3)
A 		7.4
(-2.3)
A 		10.1
(-1.6)
A 		27.4
(3)
A 		23
B 3.7
(-2.9)
A 		4.8
(-2.2)
A 		12.6
(3)
A 		3.6
(-3)
A 		9
C 6.2
(3)
A 		5.6
(-3)
A 		6.2
(3)
A 		5.6
(-3)
A 		1
avr 4.9
(-3) 		5.9
(-2.2) 		9.6
(0.9) 		12.2
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 623
1 2 3 4 5 6 range
A 298.8
(-0.3)
P 		76.7
(-2.5)
A 		32.8
(-3)
A 		29.1
(-3)
A 		339.1
(0.1)
P 		634.7
(3)
P 		606
B 265.6
(-0.5)
P 		67.6
(-2.4)
A 		11.5
(-3)
A 		78.1
(-2.3)
A 		238.7
(-0.7)
P 		609.8
(3)
P 		598
C 229.6
(-1.1)
P 		51
(-2.9)
A 		40
(-3)
A 		80.5
(-2.6)
A 		280.7
(-0.5)
P 		623.9
(3)
P 		584
avr 264.7
(-0.6) 		65.1
(-2.6) 		28.1
(-3) 		62.6
(-2.7) 		286.2
(-0.4) 		622.8
(3) 		595
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 1.8
(-2.5)
A 		1.6
(-3)
A 		2.6
(-0.5)
A 		4
(3)
A 		2
B 1.2
(-1.9)
A 		1.2
(-1.9)
A 		2.9
(3)
A 		0.8
(-3)
A 		2
C 2
(2.1)
A 		1.6
(0.2)
A 		2.2
(3)
A 		0.9
(-3)
A 		1
avr 1.7
(-1.9) 		1.5
(-3) 		2.6
(3) 		1.9
(-0.8) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 225
1 2 3 4 5 6 range
A 55.1
(-1)
P 		27.3
(-2.2)
A 		7.1
(-3)
A 		7.4
(-3)
A 		102.3
(0.9)
P 		154.5
(3)
P 		147
B 92.4
(-0.6)
P 		22.5
(-2.5)
A 		2.8
(-3)
A 		20.7
(-2.5)
A 		97
(-0.5)
P 		227.4
(3)
P 		225
C 85.1
(-0.7)
P 		15.6
(-2.8)
A 		10.2
(-3)
A 		16.2
(-2.8)
A 		90.8
(-0.6)
P 		208.8
(3)
P 		199
avr 77.5
(-0.8) 		21.8
(-2.5) 		6.7
(-3) 		14.8
(-2.7) 		96.7
(-0.2) 		196.9
(3) 		190
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193748_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15853
Sequence Type Exemplar sequence
Representative Public ID C02E7.1 NCBI
Target Description C02E7.1 /REP_DB=WormBase Gene ID /WP=CE07838 /TR=O16436 /GB=AAC24161.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=sodium-potassium-transporting ATPase alpha-2 chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071927(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071927 NCBI Caenorhabditis elegans C02E7.1 (C02E7.1) mRNA, complete cds. 11/11 None
SNAP00000015188 ENSEMBL cdna:SNAP chromosome:CEL140:V:4935501:4938899:1 11/11 None
GENEFINDER00000015199 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4935327:4938899:1 11/11 None
C02E7.1 ENSEMBL cdna:known chromosome:CEL140:V:4934552:4938899:1 gene:C02E7.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4934613-4938899(+) 99.87 99.87
Public Domain and Genome References
Gene Title sodium/potassium-transporting ATPase alpha-2 chain
Ensembl C02E7.1
Entrez Gene 182114 Entrez gene
SwissProt O16436 EMBL-EBI
WormBase CE07838 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
6812 cation transport inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
15662 ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism inferred from electronic annotation QuickGO AmiGO
16820 hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC24161 Hypothetical protein C02E7.1 [Caenorhabditis elegans] ref|NP_504328.1| C02E7.1 [Caenorhabditis elegans] 0.0
blast AAB65385 Hypothetical protein C09H5.2a [Caenorhabditis elegans] ref|NP_505083.1| C09H5.2a [Caenorhabditis elegans] 0.0
blast CAE66142 Hypothetical protein CBG11371 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A1A4_HUMAN (Q13733) Sodium/potassium-transporting ATPase alpha-4 chain (EC 3.6.3.9) (Sodium pump 4) (Na+/K+ ATPase 4) 0.0
ec A1A2_HUMAN (P50993) Sodium/potassium-transporting ATPase alpha-2 chain precursor (EC 3.6.3.9) (Sodium pump 2) (Na+/K+ ATPase 2) 0.0
ec A1A1_HUMAN (P05023) Sodium/potassium-transporting ATPase alpha-1 chain precursor (EC 3.6.3.9) (Sodium pump 1) (Na+/K+ ATPase 1) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 6.3E-55
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 1.3E-60
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 4.4E-8
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 3.2E-10
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 4.0E-26
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 6.3E-55
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 1.3E-60
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 4.4E-8
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 3.2E-10
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 2.9E-21
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 6.3E-55
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 1.3E-60
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 4.4E-8
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 3.2E-10
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 3.4E-24
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504328.1 8 102-121,126-148,292-314,329-351,816-838,876-898,976-998,1011-1028
C02E7.1 8 102-121,126-148,292-314,329-351,816-838,876-898,976-998,1011-1028
SNAP00000015188 8 59-78,83-105,249-271,286-308,773-795,833-855,933-955,968-985
GENEFINDER00000015199 8 97-116,121-143,287-309,324-346,811-833,871-893,971-993,1006-1023
Sequence
Target Sequence 
>C. ELEGANS:193748_AT
tgtatcgctagcctacgagggaccagaagcagacatcatgcttcagcctcctaggaaaaa
ggacacacatcttgtcactaaaggtcttatcacttacacgtacctgtttatgtcaatctt
catctcaattggatgtgtatgtgcatatctgctatcctactatattaatggaattgggcc
ctgggagttggcattcaccgctagcacttatttcaagcatggagctgcaaattttaccac
tgctaaaggagtcattttaaatgaagaacaacaaatgtatatggccaatcaggcagctgc
agcgttccatatctcattggttcttgggcaagcatttcatctttggatgtgtttaacccg
ccgtgtttcgatttttgctcacgggctgggaaatattgttgcaatctttgctgttatcat
tgatctccttctcatttgcatattcacctttgttccaggagtcaagtatattttcggatc
tcagccaccaccatggcagtgctggcttgtaccaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTATCGCTAGCCTACGAGGGACCA 336 559 2553 Antisense
ATCATGCTTCAGCCTCCTAGGAAAA 1 29 2587 Antisense
ATGTGCATATCTGCTATCCTACTAT 655 49 2691 Antisense
TTGGGCCCTGGGAGTTGGCATTCAC 701 685 2726 Antisense
CATTCACCGCTAGCACTTATTTCAA 576 215 2744 Antisense
GCAGCGTTCCATATCTCATTGGTTC 246 315 2851 Antisense
TCTTTGGATGTGTTTAACCCGCCGT 442 615 2892 Antisense
TGTTATCATTGATCTCCTTCTCATT 551 561 2964 Antisense
ATTTGCATATTCACCTTTGTTCCAG 665 17 2986 Antisense
AGTATATTTTCGGATCTCAGCCACC 271 63 3017 Antisense
ATGGCAGTGCTGGCTTGTACCAATT 332 53 3045 Antisense
Sequence Source Affymetrix Proprietary Database