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[193730_at]

Description DY3.4 /REP_DB=WormBase Gene ID /WP=CE15748 /CHR=1 /FEA=Sanger Annotation /DEF=Reverse transcriptase (RNA-dependent DNA polymerase) (HINXTON) TR:O45321 protein_id:CAB09413.1
Cluster 6grp_high1_10_10 > 98      6grp_total > 97      4grp_total > 03      6grp_cgc > 69      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 66.4
(-2.5)
A 		53.1
(-3)
A 		61.8
(-2.7)
A 		208.7
(3)
A 		156
B 75.4
(-1.5)
A 		60.5
(-3)
A 		60.2
(-3)
A 		120.2
(3)
P 		60
C 91.3
(-1.1)
M 		50.8
(-3)
A 		52.4
(-2.9)
P 		182.1
(3)
P 		131
avr 77.7
(-1.8) 		54.8
(-3) 		58.1
(-2.8) 		170.3
(3) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 527
1 2 3 4 5 6 range
A 61
(-3)
A 		65
(-2.9)
P 		135.4
(-1.5)
P 		224.3
(0.2)
P 		366.3
(3)
P 		322.5
(2.1)
P 		305
B 85.7
(-2.8)
A 		74.7
(-3)
A 		159.3
(-1.8)
P 		113.6
(-2.5)
P 		386.8
(1.3)
P 		509.7
(3)
P 		435
C 63.8
(-3)
P 		83.6
(-2.8)
P 		98
(-2.6)
A 		167.4
(-1.8)
P 		269.5
(-0.6)
P 		588
(3)
P 		524
avr 70.2
(-3) 		74.4
(-2.9) 		130.9
(-2.1) 		168.4
(-1.5) 		340.9
(1) 		473.4
(3) 		403
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 24.2
(1)
A 		11.5
(-3)
A 		16.2
(-1.5)
A 		30.5
(3)
A 		19
B 24
(2.2)
A 		15
(-2.5)
A 		14
(-3)
A 		25.6
(3)
P 		12
C 30.2
(2.8)
M 		14.6
(-3)
A 		18.3
(-1.6)
P 		30.7
(3)
P 		16
avr 26.1
(1.9) 		13.7
(-3) 		16.2
(-2) 		28.9
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 11.2
(-3)
A 		23.1
(-2.3)
P 		29.5
(-1.9)
P 		56.9
(-0.2)
P 		110.5
(3)
P 		78.5
(1.1)
P 		99
B 29.8
(-2.8)
A 		24.9
(-3)
A 		38.7
(-2.5)
P 		30.1
(-2.8)
P 		157.1
(1.8)
P 		190.1
(3)
P 		165
C 23.7
(-3)
P 		25.5
(-2.9)
P 		24.9
(-3)
A 		33.7
(-2.7)
P 		87.2
(-0.8)
P 		196.8
(3)
P 		173
avr 21.6
(-3) 		24.5
(-2.9) 		31
(-2.6) 		40.2
(-2.2) 		118.3
(1.3) 		155.1
(3) 		134
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193730_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1009
Sequence Type Exemplar sequence
Representative Public ID DY3.4 NCBI
Target Description DY3.4 /REP_DB=WormBase Gene ID /WP=CE15748 /CHR=1 /FEA=Sanger Annotation /DEF=Reverse transcriptase (RNA-dependent DNA polymerase) (HINXTON) TR:O45321 protein_id:CAB09413.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059972(11), NM_059973(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059972 NCBI Caenorhabditis elegans Telomerase Reverse Transcriptase family member (trt-1) (trt-1) mRNA, complete cds. 11/11 None
NM_059973 NCBI Caenorhabditis elegans Telomerase Reverse Transcriptase family member (trt-1) (trt-1) mRNA, complete cds. 11/11 None
GENEFINDER00000022850 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8776646:8779306:1 11/11 None
DY3.4a.2 ENSEMBL cdna:known chromosome:CEL140:I:8776450:8779308:1 gene:DY3.4 11/11 None
DY3.4b.4 ENSEMBL cdna:known chromosome:CEL140:I:8776644:8779306:1 gene:DY3.4 11/11 None
DY3.4a.1 ENSEMBL cdna:known chromosome:CEL140:I:8776646:8779310:1 gene:DY3.4 11/11 None
DY3.4b.3 ENSEMBL cdna:known chromosome:CEL140:I:8776646:8779306:1 gene:DY3.4 11/11 None
DY3.4b.2 ENSEMBL cdna:known chromosome:CEL140:I:8776648:8779306:1 gene:DY3.4 11/11 None
DY3.4b.1 ENSEMBL cdna:known chromosome:CEL140:I:8776652:8779306:1 gene:DY3.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022835 1/11 Cross Hyb Matching Probes None
SNAP00000022836 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8776576-8779237(+) 100.0 100.0
Public Domain and Genome References
Ensembl DY3.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB09413 Hypothetical protein DY3.4a [Caenorhabditis elegans] ref|NP_492373.1| Telomerase Reverse Transcriptase family member (trt-1) [Caenorhabditis elegans] 0.0
blast CAC42278 Hypothetical protein DY3.4b [Caenorhabditis elegans] ref|NP_492374.1| Telomerase Reverse Transcriptase family member (trt-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 0.0038
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 0.0038
Sequence
Target Sequence 
>C. ELEGANS:193730_AT
tgcacatgaggcgcttcgtcgaaaaatggaattgaaaggagtattcaattttgaagtttg
ctaccgagagatgagttcctctacaacactctacagtgttattcgtaccacactttcaac
gtactactacaagcgtggcccaacatcgtggagaattacaaaaggagttccacagggaca
tcctatatcttcaaatttagcacatatgtacctcaataactttgagcagaaatattggag
caacgaaaaagaggattcgagaattgttttctgcagatatgaggatgatttcattttcat
tacaactgaaaattctttattcgagaagatgatgaaaccattatctactggcaataacac
tcattttttgacggctaatccgaaaaagttcaagaaatcagagcgatgtggagcatcaca
agtccttcaatggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCACATGAGGCGCTTCGTCGAAAA 542 575 1170 Antisense
GTTTGCTACCGAGAGATGAGTTCCT 665 449 1225 Antisense
GATGAGTTCCTCTACAACACTCTAC 492 411 1239 Antisense
CACTCTACAGTGTTATTCGTACCAC 502 197 1256 Antisense
ACCACACTTTCAACGTACTACTACA 204 85 1276 Antisense
TACAAGCGTGGCCCAACATCGTGGA 353 641 1297 Antisense
AAGGAGTTCCACAGGGACATCCTAT 310 159 1331 Antisense
GGGACATCCTATATCTTCAAATTTA 381 495 1344 Antisense
ATCTACTGGCAATAACACTCATTTT 684 29 1512 Antisense
CACTCATTTTTTGACGGCTAATCCG 609 195 1527 Antisense
GGAGCATCACAAGTCCTTCAATGGT 358 521 1579 Antisense
Sequence Source Affymetrix Proprietary Database