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[193727_at] NADH-ubiquinone oxidoreductase 13 KD-B subunit

Description C33A12.1 /REP_DB=WormBase Gene ID /WP=CE05347 /TR=SW:Q18359 /GB=CAA92789.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase 13 KD-B subunit
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 51      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 26      6grp_total > 62      4grp_total > 49      6grp_highest_sub > 04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1604
1 2 3 4 range
A 4799
(-2.7)
P 		5125
(-0.9)
P 		5855.7
(3)
P 		4735.4
(-3)
P 		1120
B 4604.6
(-3)
P 		5396.2
(3)
P 		5231.6
(1.8)
P 		4784
(-1.6)
P 		792
C 4867.5
(-2.7)
P 		6209
(3)
P 		5581.8
(0.3)
P 		4801.2
(-3)
P 		1408
avr 4757
(-3) 		5576.7
(3) 		5556.4
(2.9) 		4773.5
(-2.9) 		820

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4093
1 2 3 4 5 6 range
A 5239.1
(-1)
P 		5303.4
(-0.9)
P 		7983.7
(3)
P 		7368.4
(2.1)
P 		4877.6
(-1.6)
P 		3890.9
(-3)
P 		4093
B 5494.3
(-0.1)
P 		5598.1
(0.1)
P 		6972
(2.7)
P 		6413.1
(1.6)
P 		7128.6
(3)
P 		4012
(-3)
P 		3117
C 5790.1
(-0.3)
P 		5798.3
(-0.3)
P 		7230.7
(3)
P 		6948.8
(2.3)
P 		6574.5
(1.5)
P 		4636.5
(-3)
P 		2594
avr 5507.8
(-0.5) 		5566.6
(-0.4) 		7395.5
(3) 		6910.1
(2.1) 		6193.6
(0.8) 		4179.8
(-3) 		3216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1252
1 2 3 4 range
A 1747.3
(3)
P 		1112.5
(-0.6)
P 		1530.8
(1.8)
P 		693.2
(-3)
P 		1054
B 1468.4
(3)
P 		1332.7
(1.2)
P 		1212.3
(-0.4)
P 		1019.5
(-3)
P 		449
C 1611.3
(1.2)
P 		1785.1
(2.2)
P 		1945.4
(3)
P 		809.6
(-3)
P 		1136
avr 1609
(3) 		1410.1
(1.4) 		1562.8
(2.6) 		840.8
(-3) 		768

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1949
1 2 3 4 5 6 range
A 965.8
(-2.9)
P 		1886.2
(3)
P 		1738.7
(2.1)
P 		1868.8
(2.9)
P 		1471.8
(0.4)
P 		947.3
(-3)
P 		939
B 1911.7
(-1.2)
P 		1863.4
(-1.4)
P 		1693
(-2.2)
P 		1698.6
(-2.1)
P 		2896.3
(3)
P 		1496.1
(-3)
P 		1400
C 2146.2
(3)
P 		1769.2
(-0)
P 		1840.3
(0.5)
P 		1397.4
(-3)
P 		2127.4
(2.8)
P 		1552.1
(-1.8)
P 		749
avr 1674.6
(-0.5) 		1839.6
(0.7) 		1757.3
(0.1) 		1654.9
(-0.7) 		2165.2
(3) 		1331.8
(-3) 		833
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193727_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9199
Sequence Type Exemplar sequence
Representative Public ID C33A12.1 NCBI
Target Description C33A12.1 /REP_DB=WormBase Gene ID /WP=CE05347 /TR=SW:Q18359 /GB=CAA92789.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase 13 KD-B subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069287(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069287 NCBI Caenorhabditis elegans C33A12.1 (C33A12.1) mRNA, complete cds. 11/11 None
SNAP00000009032 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9523847:9524867:-1 11/11 None
GENEFINDER00000009039 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9523847:9524867:-1 11/11 None
C33A12.1 ENSEMBL cdna:known chromosome:CEL140:IV:9523765:9524872:-1 gene:C33A12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9523852-9524873(-) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-ubiquinone oxidoreductase 13 KD-B subunit
Ensembl C33A12.1
Entrez Gene 177786 Entrez gene
SwissProt Q18359 EMBL-EBI
WormBase CE05347 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1601366_AT similar to NADH-ubiquinone oxidoreductase 13 kDa-B subunit (Complex I-13Kd-B) (CI-13Kd-B) (Complex I subunit B13) cfa
CANINE_2:CFA.13444.1.S1_S_AT similar to NADH-ubiquinone oxidoreductase 13 kDa-B subunit (Complex I-13Kd-B) (CI-13Kd-B) (Complex I subunit B13) cfa
CHICKEN:GGA.6336.1.S1_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa gga
HG-U133_PLUS_2:215850_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-U133A_2:215850_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-U133A:215850_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HU35KSUBA:HUM_ALU_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HU35KSUBB:HUM_ALU_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HU35KSUBC:HUM_ALU_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HU35KSUBD:HUM_ALU_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HUGENEFL:HUM_ALU_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HC-G110:HUM_ALU_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HU35KSUBB:RC_AA024664_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HU35KSUBD:RC_AA369460_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HUGENEFL:U53468_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-U133A:201304_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-U133_PLUS_2:201304_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-FOCUS:201304_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-U133A_2:201304_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
U133_X3P:G13699821_3P_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
U133_X3P:HS.287492.0.S1_3P_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
U133_X3P:215850_3P_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-U95AV2:38462_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-U95D:74839_R_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HG-U95B:44896_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
HU35KSUBB:RC_R08184_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa hs
MU11KSUBA:AA543494_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 mm
MG-U74AV2:97201_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 mm
MOE430A:1417286_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 mm
MOUSE430A_2:1417286_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 mm
MOUSE430_2:1417286_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 mm
MOE430A:1417285_A_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 mm
MOUSE430A_2:1417285_A_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 mm
MOUSE430_2:1417285_A_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 mm
RT-U34:D86215_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 5 rn
RG-U34A:D86215_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 5 rn
RAE230A:1386951_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 5 rn
RAT230_2:1386951_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 5 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5743 mitochondrial inner membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16651 oxidoreductase activity, acting on NADH or NADPH inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92789 Hypothetical protein C33A12.1 [Caenorhabditis elegans] ref|NP_501688.1| C33A12.1 [Caenorhabditis elegans] sp|Q18359|NUFM_CAEEL Putative NADH-ubiquinone oxidoreductase 17.3 kDa subunit (Complex I-17.3KD) (CI-17.3KD) 1.0E-80
blast CAE72769 Hypothetical protein CBG20020 [Caenorhabditis briggsae] 4.0E-77
blast XP_415997 PREDICTED: similar to NADH dehydrogenase [Gallus gallus] 3.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR006806 EMBL-EBI ETC complex I subunit 2.2E-36
Sequence
Target Sequence 
>C. ELEGANS:193727_AT
ggctcgtgccacacaacagagatccatgtacgagaacccttacatcaaccgtttcaaggc
caggagcaaggtttccgaggatttccacaagaagactacaggaattactggactttttgt
caatgagcatccacatcgtgcgttgactgttgtgtatggcagaattctccgcgcccttga
acaaattccacgggatgctgcttaccgcaagtatactgaagctgtggtaaagcagcgttt
ggctcttgttcaagccgaaaacgacattaagaagcttgaggagaaaattggaatgggaca
aatcgaagaagtcatcgaacaggccgaatatgaactggagacaacacgagcaattgttga
ttcaaaagcatgggagcctctcgtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTCGTGCCACACAACAGAGATCC 621 539 42 Antisense
CAGAGATCCATGTACGAGAACCCTT 693 205 58 Antisense
TACGAGAACCCTTACATCAACCGTT 355 647 70 Antisense
TCAACCGTTTCAAGGCCAGGAGCAA 382 631 86 Antisense
GCAAGGTTTCCGAGGATTTCCACAA 632 313 107 Antisense
AGCATCCACATCGTGCGTTGACTGT 180 79 167 Antisense
TGTGTATGGCAGAATTCTCCGCGCC 438 555 192 Antisense
CCGCGCCCTTGAACAAATTCCACGG 62 261 210 Antisense
CACGGGATGCTGCTTACCGCAAGTA 517 197 230 Antisense
AAAGCAGCGTTTGGCTCTTGTTCAA 651 123 270 Antisense
TCAAAAGCATGGGAGCCTCTCGTCG 214 629 403 Antisense
Sequence Source Affymetrix Proprietary Database