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[193726_at] cytochrome B5 containing fusion protein

Description T13F2.1 /REP_DB=WormBase Gene ID /WP=CE25113 /GEN=fat-4 /TR=Q9XTB7 /GB=CAB61031.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome B5 containing fusion protein
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 61      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 27      6grp_total > 61      4grp_total > 47      6grp_highest_sub > 03_04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2978
1 2 3 4 range
A 6192.3
(3)
P 		3214.1
(-3)
P 		4096.7
(-1.2)
P 		3798.1
(-1.8)
P 		2978
B 4890.5
(3)
P 		4646.1
(1.8)
P 		3653
(-3)
P 		4024.8
(-1.2)
P 		1238
C 6023.7
(3)
P 		4265.1
(-1.3)
P 		4360
(-1.1)
P 		3565.8
(-3)
P 		2458
avr 5702.2
(3) 		4041.8
(-2.2) 		4036.6
(-2.2) 		3796.2
(-3) 		1906

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6080
1 2 3 4 5 6 range
A 5103.3
(-3)
P 		6696.7
(-1.2)
P 		10349.4
(3)
P 		9502
(2)
P 		7243.9
(-0.6)
P 		5872.1
(-2.1)
P 		5246
B 6323.9
(-2.1)
P 		6794
(-1.4)
P 		9934.3
(3)
P 		9682.5
(2.6)
P 		6917.9
(-1.2)
P 		5661.8
(-3)
P 		4273
C 6088.5
(-2.3)
P 		6380.8
(-2)
P 		8587.1
(0.3)
P 		11183.5
(3)
P 		7196.8
(-1.1)
P 		5374.2
(-3)
P 		5809
avr 5838.6
(-2.7) 		6623.8
(-1.7) 		9623.6
(2.3) 		10122.7
(3) 		7119.5
(-1) 		5636
(-3) 		4487
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1699
1 2 3 4 range
A 2254.6
(3)
P 		697.7
(-2.5)
P 		1071
(-1.2)
P 		556
(-3)
P 		1699
B 1559.5
(3)
P 		1147.5
(-0.5)
P 		846.5
(-3)
P 		857.7
(-2.9)
P 		713
C 1994
(3)
P 		1226.2
(-0.3)
P 		1519.6
(1)
P 		601.3
(-3)
P 		1393
avr 1936
(3) 		1023.8
(-1.3) 		1145.7
(-0.8) 		671.7
(-3) 		1264

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1870
1 2 3 4 5 6 range
A 940.8
(-3)
P 		2381.7
(2.9)
P 		2253.9
(2.4)
P 		2409.9
(3)
P 		2185.9
(2.1)
P 		1429.7
(-1)
P 		1469
B 2200.4
(-2.2)
P 		2261.4
(-1.7)
P 		2412.3
(-0.4)
P 		2564.6
(0.9)
P 		2810.6
(3)
P 		2111.3
(-3)
P 		699
C 2256.7
(2.2)
P 		1947
(-1.3)
P 		2185.6
(1.4)
P 		2249
(2.1)
P 		2328.8
(3)
P 		1799.1
(-3)
P 		530
avr 1799.3
(-2.8) 		2196.7
(0.8) 		2283.9
(1.6) 		2407.8
(2.7) 		2441.8
(3) 		1780
(-3) 		662
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193726_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10027
Sequence Type Exemplar sequence
Representative Public ID T13F2.1 NCBI
Target Description T13F2.1 /REP_DB=WormBase Gene ID /WP=CE25113 /GEN=fat-4 /TR=Q9XTB7 /GB=CAB61031.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome B5 containing fusion protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069350(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069350 NCBI Caenorhabditis elegans FATty acid desaturase family member (fat-4) (fat-4) mRNA, complete cds. 11/11 None
SNAP00000043482 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9799445:9802046:1 11/11 None
GENEFINDER00000043487 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9799445:9802046:1 11/11 None
T13F2.1.3 ENSEMBL cdna:known chromosome:CEL140:IV:9799397:9802187:1 gene:T13F2.1 11/11 None
T13F2.1.2 ENSEMBL cdna:known chromosome:CEL140:IV:9799401:9802187:1 gene:T13F2.1 11/11 None
T13F2.1.1 ENSEMBL cdna:known chromosome:CEL140:IV:9799403:9802187:1 gene:T13F2.1 11/11 None
T13F2.1.4 ENSEMBL cdna:known chromosome:CEL140:IV:9799404:9802184:1 gene:T13F2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9799450-9802052(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome B5 containing fusion protein
Gene Symbol fat-4
Ensembl T13F2.1
Entrez Gene 177819 Entrez gene
SwissProt Q9XTB7 EMBL-EBI
WormBase CE25113 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1603228_AT similar to fatty acid desaturase 1 cfa
CANINE:1601524_AT similar to fatty acid desaturase 1 cfa
CANINE:1583633_AT similar to fatty acid desaturase 1 cfa
CANINE_2:CFA.13257.1.A1_AT similar to fatty acid desaturase 1 cfa
CANINE_2:CFAAFFX.24500.1.S1_S_AT similar to fatty acid desaturase 1 cfa
CANINE_2:CFAAFFX.24504.1.S1_X_AT similar to fatty acid desaturase 1 cfa
CANINE_2:CFAAFFX.24518.1.S1_AT similar to fatty acid desaturase 1 cfa
CHICKEN:GGA.13371.1.S1_AT similar to fatty acid desaturase 1; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1; delta-5 desaturase; delta-5 fatty acid desaturase gga
CHICKEN:GGAAFFX.4465.1.S1_AT similar to fatty acid desaturase 1; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1; delta-5 desaturase; delta-5 fatty acid desaturase gga
HG-U133A:208962_S_AT fatty acid desaturase 1 hs
HG-U133_PLUS_2:208962_S_AT fatty acid desaturase 1 hs
HG-U133A_2:208962_S_AT fatty acid desaturase 1 hs
HG-U133_PLUS_2:208963_X_AT fatty acid desaturase 1 hs
HG-U133A_2:208963_X_AT fatty acid desaturase 1 hs
HG-U133A:208963_X_AT fatty acid desaturase 1 hs
HG-U133_PLUS_2:208964_S_AT fatty acid desaturase 1 hs
HG-U133A:208964_S_AT fatty acid desaturase 1 hs
HG-U133A_2:208964_S_AT fatty acid desaturase 1 hs
HG-FOCUS:208964_S_AT fatty acid desaturase 1 hs
HU35KSUBA:W81053_AT fatty acid desaturase 1 hs
HU35KSUBA:RC_AA431773_AT fatty acid desaturase 1 hs
HU35KSUBC:RC_T51930_AT fatty acid desaturase 1 hs
HU35KSUBD:RC_AA489510_S_AT fatty acid desaturase 1 hs
U133_X3P:G12224983_3P_A_AT fatty acid desaturase 1 hs
U133_X3P:HS2.380041.1.S1_3P_S_AT fatty acid desaturase 1 hs
U133_X3P:HS.132898.0.S3_3P_A_AT fatty acid desaturase 1 hs
U133_X3P:HS.132898.0.S2_3P_A_AT fatty acid desaturase 1 hs
HG-U95AV2:39372_AT fatty acid desaturase 1 hs
HG-U95AV2:39373_AT fatty acid desaturase 1 hs
HG-U95AV2:41717_AT fatty acid desaturase 1 hs
HG-U95AV2:41718_G_AT fatty acid desaturase 1 hs
HG-U95AV2:41719_I_AT fatty acid desaturase 1 hs
HG-U95AV2:41720_R_AT fatty acid desaturase 1 hs
HG-U95E:78926_AT Fatty acid desaturase 1 hs
MOUSE430_2:1440444_AT fatty acid desaturase 1 mm
MOE430B:1440444_AT fatty acid desaturase 1 mm
MOE430A:1423680_AT fatty acid desaturase 1 mm
MOUSE430_2:1423680_AT fatty acid desaturase 1 mm
MOUSE430A_2:1423680_AT fatty acid desaturase 1 mm
MG-U74AV2:160391_AT fatty acid desaturase 1 mm
MU19KSUBA:TC14675_AT Fatty acid desaturase 1 (Fads1), mRNA mm
MU19KSUBB:TC28012_AT Fatty acid desaturase 1 (Fads1), mRNA mm
MU19KSUBB:TC28012_G_AT Fatty acid desaturase 1 (Fads1), mRNA mm
MU19KSUBB:TC28013_AT Fatty acid desaturase 1 (Fads1), mRNA mm
RAT230_2:1367857_AT fatty acid desaturase 1 rn
RAE230A:1367857_AT fatty acid desaturase 1 rn
RAE230A:1375782_AT Fatty acid desaturase 1 rn
RAT230_2:1375782_AT Fatty acid desaturase 1 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB61031 Hypothetical protein T13F2.1 [Caenorhabditis elegans] ref|NP_501751.1| FATty acid desaturase family member (fat-4) [Caenorhabditis elegans] gb|AAD13294.1| delta5-fatty acid desaturase [Caenorhabditis elegans] gb|AAC95143.1| delta 5 fatty acid desaturase [Caenorhabditis elegans] 0.0
blast H88791 protein T13F2.1 [imported] - Caenorhabditis elegans 0.0
blast CAE59987 Hypothetical protein CBG03480 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 7.4E-66
Pfam IPR001199 EMBL-EBI Cytochrome b5 1.8E-15
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501751.1 4 141-163,255-277,290-312,322-344
T13F2.1.3 4 141-163,255-277,290-312,322-344
T13F2.1.2 4 141-163,255-277,290-312,322-344
T13F2.1.1 4 141-163,255-277,290-312,322-344
T13F2.1.4 4 141-163,255-277,290-312,322-344
SNAP00000043482 4 141-163,255-277,290-312,322-344
GENEFINDER00000043487 4 141-163,255-277,290-312,322-344
Sequence
Target Sequence 
>C. ELEGANS:193726_AT
ggacattcatgttaccattcctccgtctctcgtggcttcttcagtcaatcatttttgtta
gtcagatgccaactcattattatgactattacagaaatactgcgatttatgaacaggttg
gtctctctttgcactgggcttggtcattgggtcaattgtatttcctacccgattggtcaa
ctagaataatgttcttccttgtttctcatcttgttggaggtttcctgctctctcatgtag
ttactttcaatcattattcagtggagaagtttgcattgagctcgaacatcatgtcaaatt
acgcttgtcttcaaatcatgaccacaagaaatatgagacctggaagattcattgactggc
tttggggaggtcttaactatcagattgagcaccatcttttcccaacgatgccacgacaca
acttgaacactgttatgccacttgttaaggagtttgcagcagcaaatggtttaccataca
tggtcgacgattatttcacaggattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACATTCATGTTACCATTCCTCCG 276 525 782 Antisense
ATGAACAGGTTGGTCTCTCTTTGCA 550 43 890 Antisense
CACTGGGCTTGGTCATTGGGTCAAT 582 197 913 Antisense
GTATTTCCTACCCGATTGGTCAACT 115 453 939 Antisense
GAATAATGTTCTTCCTTGTTTCTCA 454 327 965 Antisense
CATCTTGTTGGAGGTTTCCTGCTCT 385 213 988 Antisense
CCTGCTCTCTCATGTAGTTACTTTC 686 265 1005 Antisense
GATTCATTGACTGGCTTTGGGGAGG 340 431 1127 Antisense
TCAGATTGAGCACCATCTTTTCCCA 135 623 1161 Antisense
AACGATGCCACGACACAACTTGAAC 232 143 1185 Antisense
GGTCGACGATTATTTCACAGGATTC 185 503 1263 Antisense
Sequence Source Affymetrix Proprietary Database