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[193722_at] GTP-binding ADP-ribosylation factor

Description F22B5.1 /REP_DB=WormBase Gene ID /WP=CE27425 /GEN=evl-20 /TR=SW:Q19705 /GB=CAA90353.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=GTP-binding ADP-ribosylation factor
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 79      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 92      6grp_total > 69      4grp_total > 13      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 259
1 2 3 4 range
A 302
(1.9)
P 		209.2
(-1.1)
P 		149.1
(-3)
P 		335.8
(3)
P 		187
B 277.8
(0.1)
P 		176.8
(-2.5)
P 		159.4
(-3)
P 		386.1
(3)
P 		227
C 245.5
(-1.4)
P 		211.5
(-2.3)
P 		184.8
(-3)
P 		408.4
(3)
P 		224
avr 275.1
(0.1) 		199.2
(-2) 		164.4
(-3) 		376.8
(3) 		212

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 310.7
(1.6)
P 		212.3
(-3)
P 		216.1
(-2.8)
P 		238
(-1.8)
P 		305.3
(1.4)
P 		339.7
(3)
P 		127
B 307.7
(0.2)
P 		265.6
(-0.9)
P 		191.7
(-3)
P 		206
(-2.6)
P 		327.2
(0.8)
P 		407.5
(3)
P 		216
C 263.3
(-1.6)
P 		236.5
(-2.2)
P 		203.8
(-3)
P 		232.4
(-2.3)
P 		327.5
(-0.1)
P 		464.1
(3)
P 		260
avr 293.9
(-0.3) 		238.1
(-2) 		203.9
(-3) 		225.5
(-2.4) 		320
(0.5) 		403.8
(3) 		200
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 110
(3)
P 		45.4
(-2.5)
P 		39
(-3)
P 		49.2
(-2.1)
P 		71
B 88.6
(3)
P 		43.7
(-2.2)
P 		36.9
(-3)
P 		82.3
(2.3)
P 		52
C 81.3
(3)
P 		60.8
(-3)
P 		64.4
(-1.9)
P 		68.9
(-0.6)
P 		21
avr 93.3
(3) 		50
(-2.6) 		46.8
(-3) 		66.8
(-0.4) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 109
1 2 3 4 5 6 range
A 57.3
(-1.6)
P 		75.5
(0.8)
P 		47.1
(-3)
P 		60.4
(-1.2)
P 		92.1
(3)
P 		82.7
(1.7)
P 		45
B 107.1
(0.4)
P 		88.4
(-0.6)
P 		46.6
(-3)
P 		54.6
(-2.5)
P 		132.9
(1.9)
P 		152
(3)
P 		105
C 97.6
(-0.2)
P 		72.1
(-1.6)
P 		51.9
(-2.7)
P 		46.7
(-3)
P 		106
(0.3)
P 		155.3
(3)
P 		109
avr 87.3
(-0.1) 		78.7
(-0.8) 		48.5
(-3) 		53.9
(-2.6) 		110.3
(1.5) 		130
(3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193722_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2915
Sequence Type Exemplar sequence
Representative Public ID F22B5.1 NCBI
Target Description F22B5.1 /REP_DB=WormBase Gene ID /WP=CE27425 /GEN=evl-20 /TR=SW:Q19705 /GB=CAA90353.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=GTP-binding ADP-ribosylation factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063378(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063378 NCBI Caenorhabditis elegans abnormal Eversion of VuLva family member (evl-20) (evl-20) mRNA, complete cds. 10/11 None
SNAP00000010402 ENSEMBL cdna:SNAP chromosome:CEL140:II:8444229:8445027:1 10/11 None
GENEFINDER00000010413 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8444229:8445027:1 10/11 None
F22B5.1 ENSEMBL cdna:known chromosome:CEL140:II:8444229:8445027:1 gene:F22B5.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8444225-8445024(+) 100.0 100.0
Public Domain and Genome References
Gene Title GTP-binding ADP-ribosylation factor
Gene Symbol evl-20
Ensembl F22B5.1
Entrez Gene 191632 Entrez gene
SwissProt Q19705 EMBL-EBI
WormBase CE27425 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18804_AT ADP-ribosylation factor-like protein 2 (ARL2) at
ATH1-121501:265337_AT ADP-ribosylation factor-like protein 2 (ARL2) at
CANINE_2:CFAAFFX.21634.1.S1_AT similar to ADP-ribosylation factor-like protein 2 cfa
DROSOPHILA_2:1641687_AT ADP ribosylation factor 84F dm
DROSGENOME1:153118_AT ADP ribosylation factor 84F dm
HU35KSUBA:RC_AA450040_S_AT ADP-ribosylation factor-like 2 hs
HG-U133A:202564_X_AT ADP-ribosylation factor-like 2 hs
HG-U133_PLUS_2:202564_X_AT ADP-ribosylation factor-like 2 hs
HG-FOCUS:202564_X_AT ADP-ribosylation factor-like 2 hs
HG-U133A_2:202564_X_AT ADP-ribosylation factor-like 2 hs
U133_X3P:G4502228_3P_X_AT ADP-ribosylation factor-like 2 hs
U133_X3P:G4502228_3P_AT ADP-ribosylation factor-like 2 hs
HG-U95AV2:31893_AT ADP-ribosylation factor-like 2 hs
MOE430A:1422549_AT ADP-ribosylation factor-like 2 mm
MOUSE430A_2:1422549_AT ADP-ribosylation factor-like 2 mm
MOUSE430_2:1422549_AT ADP-ribosylation factor-like 2 mm
MU11KSUBB:MSA.3812.0_F_AT ADP-ribosylation factor-like 2 mm
MU11KSUBB:W41678_S_AT ADP-ribosylation factor-like 2 mm
MG-U74AV2:103854_AT ADP-ribosylation factor-like 2 mm
MOE430B:1458565_AT ADP-ribosylation factor-like 2 mm
MOUSE430_2:1458565_AT ADP-ribosylation factor-like 2 mm
MU19KSUBA:TC16099_AT ADP-ribosylation factor-like 2 (Arl2), mRNA mm
RG-U34A:RC_AI177418_AT ADP-ribosylation factor-like 2 rn
RAE230A:1368166_AT ADP-ribosylation factor-like 2 rn
RAT230_2:1368166_AT ADP-ribosylation factor-like 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90353 Hypothetical protein F22B5.1 [Caenorhabditis elegans] ref|NP_495779.1| abnormal Eversion of VuLva family member (evl-20) [Caenorhabditis elegans] sp|Q19705|ARFM_CAEEL GTP-binding ADP-ribosylation factor homolog protein evl-20 3.0E-94
blast T21237 ADP-ribosylation factor F22B5.1 [similarity] - Caenorhabditis elegans 4.0E-91
blast CAE57646 Hypothetical protein CBG00634 [Caenorhabditis briggsae] emb|CAE57073.1| Hypothetical protein CBG24969 [Caenorhabditis briggsae] 7.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR006689 EMBL-EBI ARF/SAR superfamily 5.3E-96
Sequence
Target Sequence 
>C. ELEGANS:193722_AT
atgggcttcttgaagattctccgtaagcaacgtgctcgggaacgcgagatgagaatatta
attcttggattagacaacgctggaaagactactttaatgaaaaaattcctcgacgagccg
actgatacaattgaaccaacattaggatttgatatcaaaaccgtgcatttcaaagatttt
caactaaacttatgggatgttggcggacagaaaagtctaagatcttattggaagaattat
ttcgaatccacagatgctttaatttgggtggtagattcgtcagatagagagcgtcttcta
caatgctccgaagaactcaaaaaattgctcggagaagaacgattagccggtgcttctctt
ctagttcttgccaacaaaagtgatctgccaggggccattgatgtgaattcaatagcacag
gtaattcagatgtcttcagtatatacttccgaaaagaaatgctttcttgtactcgatctc
cattccatcaaatcccatcactggaagatctttagttgctgtgcactttctggtgatcga
ctggtccaagcaatgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGGCTTCTTGAAGATTCTCCGTA 179 51 13 Antisense
GATTCTCCGTAAGCAACGTGCTCGG 369 431 27 Antisense
TCGACGAGCCGACTGATACAATTGA 525 601 122 Antisense
GAGCGTCTTCTACAATGCTCCGAAG 621 383 301 Antisense
AAGAACGATTAGCCGGTGCTTCTCT 350 153 347 Antisense
TTCTCTTCTAGTTCTTGCCAACAAA 693 691 366 Antisense
GTGATCTGCCAGGGGCCATTGATGT 115 485 392 Antisense
GAAATGCTTTCTTGTACTCGATCTC 592 361 468 Antisense
GGAAGATCTTTAGTTGCTGTGCACT 589 531 515 Antisense
GCTGTGCACTTTCTGGTGATCGACT 133 295 530 Antisense
GTGATCGACTGGTCCAAGCAATGAC 20 483 545 Antisense
Sequence Source Affymetrix Proprietary Database