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[193718_at]

Description C15B12.7 /REP_DB=WormBase Gene ID /WP=CE27065 /GEN=cdf-1 /TR=Q18009 /GB=AAK39165.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Cobalt uptake protein (S. cerevisiae)
Cluster 6grp_highest_10_10 > 53      6grp_high1_10_10 > 35      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 71      6grp_total > 36      4grp_total > 62      6grp_highest_sub > 02      6grp_cgc > 25      6grp_high1_sub > 02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 264
1 2 3 4 range
A 472.7
(3)
P 		299.5
(-3)
P 		440.3
(1.9)
P 		305.9
(-2.8)
P 		173
B 405.6
(3)
P 		333.5
(0.8)
P 		285.1
(-0.7)
P 		208.7
(-3)
P 		197
C 416.3
(3)
P 		331.1
(-1.9)
P 		401.7
(2.2)
P 		312.9
(-3)
P 		103
avr 431.5
(3) 		321.4
(-1.2) 		375.7
(0.8) 		275.8
(-3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 355.2
(0.6)
P 		414.8
(3)
P 		268.4
(-3)
P 		347.6
(0.2)
P 		320.3
(-0.9)
P 		273.9
(-2.8)
P 		146
B 330.2
(-0.1)
P 		376.6
(2.4)
P 		304.5
(-1.6)
P 		303
(-1.7)
P 		278.9
(-3)
P 		386.8
(3)
P 		108
C 374.7
(1)
P 		428.5
(3)
P 		334.7
(-0.5)
P 		400
(1.9)
P 		269.7
(-3)
P 		363.4
(0.5)
P 		159
avr 353.4
(0.3) 		406.6
(3) 		302.5
(-2.3) 		350.2
(0.1) 		289.6
(-3) 		341.4
(-0.3) 		117
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 172.1
(3)
P 		65
(-2)
P 		115.1
(0.3)
P 		44.8
(-3)
P 		127
B 129.3
(3)
P 		82.4
(-0.3)
P 		66.1
(-1.5)
P 		44.5
(-3)
P 		85
C 137.8
(2.8)
P 		95.2
(-0.1)
P 		140
(3)
P 		52.8
(-3)
P 		87
avr 146.4
(3) 		80.9
(-1) 		107.1
(0.6) 		47.4
(-3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 65.5
(-2.5)
P 		147.5
(3)
P 		58.4
(-3)
P 		88.2
(-1)
P 		96.6
(-0.4)
P 		66.7
(-2.4)
P 		89
B 114.9
(0.5)
P 		125.4
(1.4)
P 		74
(-3)
P 		80.3
(-2.5)
P 		113.3
(0.4)
P 		144.2
(3)
P 		70
C 138.9
(3)
P 		130.7
(2.2)
P 		85.2
(-2.5)
P 		80.4
(-3)
P 		87.3
(-2.3)
P 		121.6
(1.2)
P 		59
avr 106.4
(0.3) 		134.5
(3) 		72.5
(-3) 		83
(-2) 		99.1
(-0.4) 		110.8
(0.7) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193718_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17020
Sequence Type Exemplar sequence
Representative Public ID C15B12.7 NCBI
Target Description C15B12.7 /REP_DB=WormBase Gene ID /WP=CE27065 /GEN=cdf-1 /TR=Q18009 /GB=AAK39165.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Cobalt uptake protein (S. cerevisiae)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076695(11), NM_076694(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076695 NCBI Caenorhabditis elegans Cation Diffusion Facilitator family member (cdf-1) (cdf-1) mRNA, complete cds. 11/11 None
NM_076694 NCBI Caenorhabditis elegans Cation Diffusion Facilitator family member (cdf-1) (cdf-1) mRNA, complete cds. 11/11 None
SNAP00000029087 ENSEMBL cdna:SNAP chromosome:CEL140:X:6500250:6502932:-1 11/11 None
GENEFINDER00000029090 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6500250:6504568:-1 11/11 None
C15B12.7b ENSEMBL cdna:known chromosome:CEL140:X:6499892:6504557:-1 gene:C15B12.7 11/11 A 
C15B12.7a.2 ENSEMBL cdna:known chromosome:CEL140:X:6500250:6504570:-1 gene:C15B12.7 11/11 None
C15B12.7a.1 ENSEMBL cdna:known chromosome:CEL140:X:6500250:6504568:-1 gene:C15B12.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6500249-6504493(-) 98.73 100.0
Public Domain and Genome References
Ensembl C15B12.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL13323 Cation diffusion facilitator family protein 1, isoform b [Caenorhabditis elegans] ref|NP_509096.1| Cation Diffusion Facilitator family member (cdf-1) [Caenorhabditis elegans] 0.0
blast AAK39165 Cation diffusion facilitator family protein 1, isoform a [Caenorhabditis elegans] ref|NP_509095.1| Cation Diffusion Facilitator family member (cdf-1) [Caenorhabditis elegans] 0.0
blast T15506 hypothetical protein C15B12.7 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002524 EMBL-EBI Cation efflux protein 4.8E-53
Pfam IPR002524 EMBL-EBI Cation efflux protein 4.8E-53
Pfam IPR002524 EMBL-EBI Cation efflux protein 4.8E-53
Pfam IPR002524 EMBL-EBI Cation efflux protein 4.6E-10
Pfam IPR002524 EMBL-EBI Cation efflux protein 6.5E-33
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509096.1 6 117-139,143-160,180-202,212-234,387-409,419-441
NP_509095.1 6 75-97,101-118,138-160,170-192,345-367,377-399
C15B12.7b 6 117-139,143-160,180-202,212-234,387-409,419-441
C15B12.7a.2 6 75-97,101-118,138-160,170-192,345-367,377-399
C15B12.7a.1 6 75-97,101-118,138-160,170-192,345-367,377-399
SNAP00000029087 6 68-90,94-111,131-153,163-185,338-360,370-392
GENEFINDER00000029090 6 100-122,127-149,193-215,225-247,400-422,432-454
Sequence
Target Sequence 
>C. ELEGANS:193718_AT
acattcacggagtttggcttcatttattgtcggatgccttcggatctgtgatcgtcatga
tttctgccggatttgtatactttttgccaacatggaaaattgccgcttatttggatccaa
ttttaagtatttccctggcctccatcatgggtttcactgcagtggttcttgtcaaaacat
cgggagaaaagctgctcaagcaaaccccagaagggctcgacctcgaaaaggtgaaaaaag
atttgtgcagcattgttggtgtatccaaagtggaaaagctgtctgtgtggacgttgtgtg
ggcaacgaatcattgctgccgcccacgtgaacatatgtcacccagcagtttttcctgaag
ctgcttataaaattaaaaattatttccacgacttgggagtacactcaacgactattgaac
ccacctttgaggatacctgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATTCACGGAGTTTGGCTTCATTT 174 105 1127 Antisense
GGCTTCATTTATTGTCGGATGCCTT 607 537 1142 Antisense
GGATGCCTTCGGATCTGTGATCGTC 465 513 1158 Antisense
GTGATCGTCATGATTTCTGCCGGAT 247 485 1174 Antisense
TGCCGGATTTGTATACTTTTTGCCA 448 585 1191 Antisense
CTCCATCATGGGTTTCACTGCAGTG 611 235 1266 Antisense
AGCAAACCCCAGAAGGGCTCGACCT 85 73 1325 Antisense
GTCTGTGTGGACGTTGTGTGGGCAA 606 471 1407 Antisense
TGGGCAACGAATCATTGCTGCCGCC 2 555 1425 Antisense
GAACATATGTCACCCAGCAGTTTTT 180 351 1455 Antisense
GAACCCACCTTTGAGGATACCTGCA 631 343 1543 Antisense
Sequence Source Affymetrix Proprietary Database