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[193710_at] eukaryotic peptide chain release factor subunit 1

Description T05H4.6 /REP_DB=WormBase Gene ID /WP=CE13279 /CHR=5 /FEA=Sanger Annotation /DEF=eukaryotic peptide chain release factor subunit 1 (ST.LOUIS) TR:O16520 protein_id:AAB66012.1
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 05      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 51      6grp_total > 04      4grp_total > 59      6grp_highest_sub > De      6grp_high1_sub > De      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 745
1 2 3 4 range
A 890.2
(2)
P 		796.2
(1)
P 		977.1
(3)
P 		438.8
(-3)
P 		538
B 838.7
(0.5)
P 		975.6
(1.7)
P 		1127.5
(3)
P 		425
(-3)
P 		703
C 807.1
(-0.6)
P 		1170.3
(3)
P 		1162.8
(2.9)
P 		564.1
(-3)
P 		606
avr 845.3
(0.6) 		980.7
(1.9) 		1089.1
(3) 		476
(-3) 		613

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 534
1 2 3 4 5 6 range
A 603.9
(-0.7)
P 		787.2
(3)
P 		670.8
(0.6)
P 		707
(1.4)
P 		490.7
(-3)
P 		526.6
(-2.3)
P 		297
B 601.1
(3)
P 		551.1
(1.2)
P 		443.6
(-2.7)
P 		513.2
(-0.2)
P 		576.4
(2.1)
P 		435
(-3)
P 		166
C 968.6
(3)
P 		671.3
(-0.8)
P 		630.3
(-1.3)
P 		545.9
(-2.4)
P 		575.5
(-2)
P 		496.6
(-3)
P 		472
avr 724.5
(3) 		669.9
(1.6) 		581.6
(-0.6) 		588.7
(-0.4) 		547.5
(-1.5) 		486.1
(-3) 		238
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 341
1 2 3 4 range
A 324.1
(3)
P 		172.8
(-0.5)
P 		255.4
(1.4)
P 		64.2
(-3)
P 		260
B 267.5
(3)
P 		240.9
(2.1)
P 		261.3
(2.8)
P 		90.6
(-3)
P 		177
C 267.2
(0.3)
P 		336.5
(1.7)
P 		405.3
(3)
P 		95.1
(-3)
P 		310
avr 286.3
(2.4) 		250.1
(1.5) 		307.3
(3) 		83.3
(-3) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 111.3
(-3)
P 		280
(3)
P 		146.1
(-1.8)
P 		179.3
(-0.6)
P 		148.1
(-1.7)
P 		128.2
(-2.4)
P 		169
B 209.1
(1.8)
P 		183.4
(0.6)
P 		107.7
(-3)
P 		135.9
(-1.7)
P 		234.2
(3)
P 		162.2
(-0.4)
P 		127
C 359
(3)
P 		204.8
(-0.7)
P 		160.4
(-1.8)
P 		109.8
(-3)
P 		186.2
(-1.2)
P 		166.2
(-1.6)
P 		249
avr 226.5
(3) 		222.7
(2.7) 		138.1
(-3) 		141.7
(-2.8) 		189.5
(0.5) 		152.2
(-2) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193710_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11629
Sequence Type Exemplar sequence
Representative Public ID T05H4.6 NCBI
Target Description T05H4.6 /REP_DB=WormBase Gene ID /WP=CE13279 /CHR=5 /FEA=Sanger Annotation /DEF=eukaryotic peptide chain release factor subunit 1 (ST.LOUIS) TR:O16520 protein_id:AAB66012.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028936(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028936 NCBI Caenorhabditis elegans T05H4.6 (T05H4.6) mRNA, complete cds. 11/11 None
SNAP00000040308 ENSEMBL cdna:SNAP chromosome:CEL140:V:6436754:6438225:1 11/11 None
GENEFINDER00000040316 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6436754:6438225:1 11/11 None
T05H4.6b.2 ENSEMBL cdna:known chromosome:CEL140:V:6436722:6438976:1 gene:T05H4.6 10/11 None
T05H4.6b.1 ENSEMBL cdna:known chromosome:CEL140:V:6436724:6438976:1 gene:T05H4.6 10/11 None
T05H4.6a.1 ENSEMBL cdna:known chromosome:CEL140:V:6436754:6438986:1 gene:T05H4.6 11/11 A 
T05H4.6a.2 ENSEMBL cdna:known chromosome:CEL140:V:6436754:6438977:1 gene:T05H4.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6436753-6438225(+) 100.0 100.0
Public Domain and Genome References
Gene Title eukaryotic peptide chain release factor subunit 1
Ensembl T05H4.6
Entrez Gene 179028 Entrez gene
SwissProt O16520 EMBL-EBI
WormBase CE13279 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261204_S_AT eukaryotic release factor 1 family protein / eRF1 family protein at
ATGENOME1:19690_S_AT eukaryotic release factor 1 family protein / eRF1 family protein at
CANINE_2:CFAAFFX.23648.1.S1_S_AT similar to eukaryotic translation termination factor 1 (predicted) cfa
CANINE_2:CFAAFFX.2814.1.S1_S_AT similar to eukaryotic translation termination factor 1 (predicted) cfa
CANINE_2:CFA.17298.1.S1_AT similar to eukaryotic translation termination factor 1 (predicted) cfa
DROSOPHILA_2:1640288_S_AT eukaryotic release factor 1 dm
DROSGENOME1:154148_AT eukaryotic release factor 1 dm
CHICKEN:GGA.6429.1.S1_S_AT eukaryotic translation termination factor 1 gga
HG-FOCUS:201574_AT eukaryotic translation termination factor 1 hs
HG-U133A:201574_AT eukaryotic translation termination factor 1 hs
HG-U133A_2:201574_AT eukaryotic translation termination factor 1 hs
HG-U133_PLUS_2:201574_AT eukaryotic translation termination factor 1 hs
HG-U133A:201573_S_AT eukaryotic translation termination factor 1 hs
HG-U133A_2:201573_S_AT eukaryotic translation termination factor 1 hs
HG-U133_PLUS_2:201573_S_AT eukaryotic translation termination factor 1 hs
HUGENEFL:X81625_AT eukaryotic translation termination factor 1 hs
HUGENEFL:M75715_S_AT eukaryotic translation termination factor 1 hs
U133_X3P:G4759033_3P_AT eukaryotic translation termination factor 1 hs
U133_X3P:G338686_3P_A_AT eukaryotic translation termination factor 1 hs
HG-U95AV2:37318_AT eukaryotic translation termination factor 1 hs
MOE430A:1424013_AT eukaryotic translation termination factor 1 mm
MOUSE430_2:1424013_AT eukaryotic translation termination factor 1 mm
MOUSE430A_2:1424013_AT eukaryotic translation termination factor 1 mm
MG-U74AV2:98608_AT eukaryotic translation termination factor 1 mm
MOUSE430_2:1451208_AT eukaryotic translation termination factor 1 mm
MOE430A:1451208_AT eukaryotic translation termination factor 1 mm
MOUSE430A_2:1451208_AT eukaryotic translation termination factor 1 mm
MU11KSUBA:AA537143_S_AT eukaryotic translation termination factor 1 mm
MU11KSUBA:D87691_S_AT eukaryotic translation termination factor 1 mm
MG-U74AV2:161344_R_AT eukaryotic translation termination factor 1 mm
MG-U74AV2:160451_AT eukaryotic translation termination factor 1 mm
MG-U74CV2:170702_AT eukaryotic translation termination factor 1 mm
MG-U74CV2:169276_AT eukaryotic translation termination factor 1 mm
MOE430A:1420023_AT eukaryotic translation termination factor 1 mm
MOUSE430A_2:1420023_AT eukaryotic translation termination factor 1 mm
MOUSE430_2:1420023_AT eukaryotic translation termination factor 1 mm
MOE430A:1420024_S_AT eukaryotic translation termination factor 1 mm
MOUSE430A_2:1420024_S_AT eukaryotic translation termination factor 1 mm
MOUSE430_2:1420024_S_AT eukaryotic translation termination factor 1 mm
MOE430B:1440629_AT eukaryotic translation termination factor 1 mm
MOUSE430_2:1440629_AT eukaryotic translation termination factor 1 mm
MG-U74AV2:96754_S_AT Eukaryotic translation termination factor 1 (Etf1), mRNA mm
MG-U74AV2:96755_AT Eukaryotic translation termination factor 1 (Etf1), mRNA mm
MU11KSUBA:AA061574_AT Eukaryotic translation termination factor 1 (Etf1), mRNA mm
MU11KSUBA:AA061574_G_AT Eukaryotic translation termination factor 1 (Etf1), mRNA mm
MU19KSUBB:TC31319_AT Eukaryotic translation termination factor 1 (Etf1), mRNA mm
RG-U34C:RC_AI180170_AT eukaryotic translation termination factor 1 (predicted) rn
RAE230A:1389038_AT eukaryotic translation termination factor 1 (predicted) rn
RAT230_2:1389038_AT eukaryotic translation termination factor 1 (predicted) rn
YEAST_2:1771279_AT Polypeptide release factor involved in translation termination; mutant form acts as a recessive omnipotent suppressor Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6415 translational termination inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16149 translation release factor activity, codon specific inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_001024107 T05H4.6 [Caenorhabditis elegans] gb|AAB66012.1| Hypothetical protein T05H4.6 [Caenorhabditis elegans] sp|O16520|ERF1_CAEEL Eukaryotic peptide chain release factor subunit 1 (eRF1) (Eukaryotic release factor 1) 0.0
blast CAE71879 Hypothetical protein CBG18934 [Caenorhabditis briggsae] 0.0
blast EAA14616 ENSANGP00000018843 [Anopheles gambiae str. PEST] ref|XP_319502.1| ENSANGP00000018843 [Anopheles gambiae str. PEST] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005141 EMBL-EBI eRF1 domain 2 3.2E-69
Pfam IPR005142 EMBL-EBI eRF1 domain 3 6.5E-76
Pfam IPR005140 EMBL-EBI eRF1 domain 1 9.0E-67
Sequence
Target Sequence 
>C. ELEGANS:193710_AT
ttgaacttgctgccgatacgctggcaagtgttaagttcattcaggaaaagaaactgattg
gtggatatttcgatgaaatcagccaagacactggaaaatacgttttcggagttaaagaca
ctcttgccgcacttgaaatgggagctattgaaactctcatctgctgggagaatttggata
ttgttcggtacaagatgaaaaattcactcggagaggacattcttctgaatctgagacctg
acgaggagaaggacaaatctcatttcaccgacagtgaaactggacaagatatggaaatta
ttgagactatgccacttttggaatggtttgctaataactacaagaacttcggagcagctt
tggaaatcgtcacagataagtcgcaagaaggagcccagtttgttcgaggattcggaggta
tcggaggattgctccgttatcgcgttgacttggctcatgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAACTTGCTGCCGATACGCTGGC 281 703 839 Antisense
ACACTCTTGCCGCACTTGAAATGGG 242 111 956 Antisense
GCTATTGAAACTCTCATCTGCTGGG 99 305 982 Antisense
TCACTCGGAGAGGACATTCTTCTGA 77 627 1042 Antisense
CATTCTTCTGAATCTGAGACCTGAC 194 217 1056 Antisense
GAAGGACAAATCTCATTTCACCGAC 215 333 1086 Antisense
TTATTGAGACTATGCCACTTTTGGA 287 675 1136 Antisense
GGAGCAGCTTTGGAAATCGTCACAG 76 519 1189 Antisense
GGAGCCCAGTTTGTTCGAGGATTCG 395 519 1228 Antisense
CGGAGGATTGCTCCGTTATCGCGTT 4 243 1260 Antisense
TTATCGCGTTGACTTGGCTCATGTA 271 681 1275 Antisense
Sequence Source Affymetrix Proprietary Database