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[193700_s_at]

Description T14G10.2 /REP_DB=WormBase Gene ID /WP=CE06448 /CHR=4 /FEA=Sanger Annotation /DEF=locus:pxf-1 cell division control protein 25 like (HINXTON) TR:Q21218 protein_id:CAA93100.1
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 31      6grp_total > 26      4grp_total > 38      6grp_highest_sub > 01_02      6grp_cgc > 14      6grp_high1_sub > 01_02      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 548
1 2 3 4 range
A 1001.5
(3)
P 		605.9
(-2.4)
P 		563.8
(-3)
P 		676
(-1.5)
P 		438
B 1067.7
(3)
P 		662.4
(-1.9)
P 		576.1
(-3)
P 		730.2
(-1.1)
P 		492
C 886.5
(3)
P 		680.5
(-0.4)
P 		519.7
(-3)
P 		608.2
(-1.6)
P 		367
avr 985.2
(3) 		649.6
(-1.7) 		553.2
(-3) 		671.5
(-1.4) 		432

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 641
1 2 3 4 5 6 range
A 1218
(3)
P 		1108.1
(2)
P 		576.9
(-3)
P 		686.1
(-2)
P 		909
(0.1)
P 		889.9
(-0.1)
P 		641
B 1201.1
(3)
P 		1174.8
(2.7)
P 		625.2
(-3)
P 		890.4
(-0.2)
P 		711.2
(-2.1)
P 		751.8
(-1.7)
P 		576
C 855.3
(-1.4)
P 		1217.1
(3)
P 		791.4
(-2.1)
P 		799.8
(-2)
P 		731
(-2.9)
P 		720.7
(-3)
P 		496
avr 1091.5
(2.1) 		1166.7
(3) 		664.5
(-3) 		792.1
(-1.5) 		783.7
(-1.6) 		787.5
(-1.5) 		502
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 266
1 2 3 4 range
A 364.6
(3)
P 		131.5
(-2.3)
P 		147.4
(-1.9)
P 		99
(-3)
P 		266
B 340.5
(3)
P 		163.6
(-2.1)
P 		133.5
(-3)
P 		155.6
(-2.4)
P 		207
C 293.5
(3)
P 		195.7
(-0.1)
P 		181.1
(-0.5)
P 		102.5
(-3)
P 		191
avr 332.9
(3) 		163.6
(-1.7) 		154
(-2) 		119
(-3) 		214

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 292
1 2 3 4 5 6 range
A 224.5
(-0.8)
P 		394.1
(3)
P 		125.6
(-3)
P 		174
(-1.9)
P 		274.3
(0.3)
P 		216.7
(-1)
P 		269
B 417.9
(3)
P 		391.1
(2.4)
P 		151.8
(-3)
P 		235.8
(-1.1)
P 		289
(0.1)
P 		280.3
(-0.1)
P 		266
C 317
(1.5)
P 		371.4
(3)
P 		201.4
(-1.8)
P 		160.8
(-3)
P 		236.5
(-0.8)
P 		241.3
(-0.7)
P 		211
avr 319.8
(1.3) 		385.5
(3) 		159.6
(-3) 		190.2
(-2.2) 		266.6
(-0.2) 		246.1
(-0.7) 		226
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193700_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8685
Sequence Type Exemplar sequence
Representative Public ID T14G10.2 NCBI
Target Description T14G10.2 /REP_DB=WormBase Gene ID /WP=CE06448 /CHR=4 /FEA=Sanger Annotation /DEF=locus:pxf-1 cell division control protein 25 like (HINXTON) TR:Q21218 protein_id:CAA93100.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028219(10), NM_001028218(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028219 NCBI Caenorhabditis elegans PDZ eXchange Factor family member (pxf-1) (pxf-1) mRNA, complete cds. 10/11 None
NM_001028218 NCBI Caenorhabditis elegans PDZ eXchange Factor family member (pxf-1) (pxf-1) mRNA, complete cds. 11/11 None
T14G10.2b.1 ENSEMBL cdna:known chromosome:CEL140:IV:10164302:10174301:-1 gene:T14G10.2 10/11 None
T14G10.2a.1 ENSEMBL cdna:known chromosome:CEL140:IV:10164352:10174301:-1 gene:T14G10.2 11/11 None
T14G10.2a.2 ENSEMBL cdna:known chromosome:CEL140:IV:10164415:10174301:-1 gene:T14G10.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
T14G10.2b.2 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10164585-10170276(-) 100.0 100.0
Public Domain and Genome References
Ensembl T14G10.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL09434 PXF isoform B [Caenorhabditis elegans] emb|CAC42313.1| Hypothetical protein T14G10.2b [Caenorhabditis elegans] emb|CAC42342.1| Hypothetical protein T14G10.2b [Caenorhabditis elegans] ref|NP_001023390.1| PDZ eXchange Factor family member (pxf-1) [Caenorhabditis elegans] 0.0
blast AAL09433 PXF isoform A [Caenorhabditis elegans] emb|CAA93519.2| Hypothetical protein T14G10.2a [Caenorhabditis elegans] emb|CAA93100.2| Hypothetical protein T14G10.2a [Caenorhabditis elegans] ref|NP_001023389.1| PDZ eXchange Factor family member (pxf-1) [Caenorhabditis elegans] gb|AAF22963.1| RA-GEF [Caenorhabditis elegans] 0.0
blast CAE59918 Hypothetical protein CBG03403 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.3E-5
Pfam IPR000159 EMBL-EBI RA domain 1.2E-22
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 1.5E-44
Pfam IPR000595 EMBL-EBI Cyclic nucleotide-binding domain 1.7E-4
Sequence
Target Sequence 
>C. ELEGANS:193700_S_AT
gcaagatcctctcttcaacggaatatgccacgtgtaactggtcgacaagcgacatcctcg
gctcaagggccagttcaactgaatgaagagacgtctacggttacgacatattatcaatca
gataatgggcgccggcaacgatctggatccgaaggaagatttgataatattccgccatca
accttctatctaacatccgacggtcttactgtatcacccagacaatcgttgtctgttgtg
attccaacacatcctcatggccactctccaacatcgccacggtgccgctcgcgatcaccg
gcatcttccgggtgttcttccttctccaccattgcctcaatcgccgctacctcgatggca
gccgcaccttcggcatttgtctccaatccgtaccaacaccaccaaaccgtccgtggacat
gtcataggacatcgtccaatgccaatcgtcactagtggttcagccacactcccgaatcat
gtttctccacgaggtttaccaccgaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAGATCCTCTCTTCAACGGAATA 484 319 3319 Antisense
GCCACGTGTAACTGGTCGACAAGCG 263 279 3345 Antisense
CTCGGCTCAAGGGCCAGTTCAACTG 405 231 3375 Antisense
ATCAGATAATGGGCGCCGGCAACGA 301 25 3435 Antisense
ATCAACCTTCTATCTAACATCCGAC 194 27 3495 Antisense
ACATCCGACGGTCTTACTGTATCAC 75 105 3511 Antisense
CTTCGGCATTTGTCTCCAATCCGTA 528 219 3686 Antisense
ACCACCAAACCGTCCGTGGACATGT 243 37 3716 Antisense
GTCCAATGCCAATCGTCACTAGTGG 189 473 3752 Antisense
TCACTAGTGGTTCAGCCACACTCCC 464 625 3767 Antisense
TCTCCACGAGGTTTACCACCGAAAT 526 591 3802 Antisense
Sequence Source Affymetrix Proprietary Database