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[193698_at] glucosamine-fructose-6-phosphate aminotransferase

Description F22B3.4 /REP_DB=WormBase Gene ID /WP=CE03255 /TR=Q19699 /GB=CAA92735.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glucosamine-fructose-6-phosphate aminotransferase
Cluster 6grp_high1_10_10 > 88      6grp_total > 98      4grp_total > 41      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 27.8
(-3)
A 		138.4
(1.9)
P 		130.3
(1.5)
P 		164.6
(3)
A 		137
B 93.4
(-3)
P 		143.2
(3)
P 		139.4
(2.5)
P 		94.7
(-2.8)
A 		50
C 91.2
(-2)
A 		162.4
(3)
P 		141.5
(1.5)
P 		77.6
(-3)
A 		85
avr 70.8
(-3) 		148
(3) 		137.1
(2.2) 		112.3
(0.2) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2194
1 2 3 4 5 6 range
A 41
(-3)
A 		82.3
(-2.9)
P 		94.4
(-2.9)
P 		247.3
(-2.4)
P 		1148.4
(0)
P 		2234.7
(3)
P 		2194
B 64.3
(-3)
P 		103.4
(-2.9)
P 		133
(-2.8)
P 		260.2
(-2.3)
P 		1095.1
(0.8)
P 		1713.3
(3)
P 		1649
C 45.4
(-3)
A 		116.6
(-2.8)
P 		140
(-2.7)
P 		269.7
(-2.3)
P 		1341.2
(1.1)
P 		1937.1
(3)
P 		1892
avr 50.2
(-3) 		100.8
(-2.8) 		122.5
(-2.8) 		259.1
(-2.3) 		1194.9
(0.6) 		1961.7
(3) 		1912
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 10.1
(-3)
A 		30
(2)
P 		34.1
(3)
P 		24.1
(0.5)
A 		24
B 29.8
(0.8)
P 		35.4
(3)
P 		32.3
(1.8)
P 		20.2
(-3)
A 		15
C 30.2
(-0.2)
A 		46.7
(2.6)
P 		49.3
(3)
P 		13.1
(-3)
A 		36
avr 23.4
(-1.7) 		37.4
(2.6) 		38.6
(3) 		19.1
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 641
1 2 3 4 5 6 range
A 7.6
(-3)
A 		29.3
(-2.8)
P 		20.6
(-2.9)
P 		62.7
(-2.4)
P 		346.5
(0.8)
P 		544.1
(3)
P 		537
B 22.4
(-3)
P 		34.4
(-2.9)
P 		32.3
(-2.9)
P 		68.9
(-2.5)
P 		444.9
(1.1)
P 		638.9
(3)
P 		617
C 16.8
(-3)
A 		35.6
(-2.8)
P 		35.6
(-2.8)
P 		54.2
(-2.6)
P 		434
(1)
P 		648.4
(3)
P 		632
avr 15.6
(-3) 		33.1
(-2.8) 		29.5
(-2.9) 		61.9
(-2.5) 		408.5
(1) 		610.5
(3) 		595
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193698_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9287
Sequence Type Exemplar sequence
Representative Public ID F22B3.4 NCBI
Target Description F22B3.4 /REP_DB=WormBase Gene ID /WP=CE03255 /TR=Q19699 /GB=CAA92735.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glucosamine-fructose-6-phosphate aminotransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069755(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069755 NCBI Caenorhabditis elegans F22B3.4 (F22B3.4) mRNA, complete cds. 11/11 None
SNAP00000028588 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11410484:11412816:1 11/11 None
GENEFINDER00000028600 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11410484:11412816:1 11/11 None
F22B3.4 ENSEMBL cdna:known chromosome:CEL140:IV:11410456:11412955:1 gene:F22B3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11410489-11412822(+) 100.0 100.0
Public Domain and Genome References
Gene Title glucosamine-fructose-6-phosphate aminotransferase
Ensembl F22B3.4
Entrez Gene 178067 Entrez gene
SwissProt Q19699 EMBL-EBI
WormBase CE03255 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256911_AT glucosamine--fructose-6-phosphate aminotransferase (isomerizing), putative / hexosephosphate aminotransferase, putative / glucosamine-6-phosphate synthase, putative / D-fructose-6-phosphate amidotransferase, putative / GLCN6P synthase, putative at
CANINE:1589341_AT similar to glutamine-fructose-6-phosphate transaminase 2 cfa
CANINE_2:CFA.12553.1.A1_S_AT similar to glutamine-fructose-6-phosphate transaminase 2 cfa
CANINE_2:CFAAFFX.1631.1.S1_S_AT similar to glutamine-fructose-6-phosphate transaminase 2 cfa
DROSOPHILA_2:1635153_AT Glutamine:fructose-6-phosphate aminotransferase 2 dm
DROSGENOME1:152464_AT Glutamine:fructose-6-phosphate aminotransferase 2 dm
CHICKEN:GGAAFFX.8765.1.S1_AT similar to glutamine-fructose-6-phosphate transaminase 2; glutamine: fructose-6-phosphate aminotransferase 2 gga
CHICKEN:GGAAFFX.8765.3.S1_AT similar to glutamine-fructose-6-phosphate transaminase 2; glutamine: fructose-6-phosphate aminotransferase 2 gga
CHICKEN:GGAAFFX.8765.3.S1_S_AT similar to glutamine-fructose-6-phosphate transaminase 2; glutamine: fructose-6-phosphate aminotransferase 2 gga
HG-U133_PLUS_2:205100_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-U133A:205100_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-FOCUS:205100_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-U133A_2:205100_AT glutamine-fructose-6-phosphate transaminase 2 hs
HU35KSUBA:RC_AA037803_AT glutamine-fructose-6-phosphate transaminase 2 hs
U133_X3P:G4826741_3P_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-U95AV2:39640_AT glutamine-fructose-6-phosphate transaminase 2 hs
HG-U133_PLUS_2:237352_AT Glutamine-fructose-6-phosphate transaminase 2 hs
HG-U133B:237352_AT Glutamine-fructose-6-phosphate transaminase 2 hs
HG-U95D:71881_R_AT Glutamine-fructose-6-phosphate transaminase 2 hs
U133_X3P:HS.220741.0.A1_3P_AT Glutamine-fructose-6-phosphate transaminase 2 hs
MOE430A:1418753_AT glutamine fructose-6-phosphate transaminase 2 mm
MOUSE430A_2:1418753_AT glutamine fructose-6-phosphate transaminase 2 mm
MOUSE430_2:1418753_AT glutamine fructose-6-phosphate transaminase 2 mm
MG-U74AV2:103737_AT glutamine fructose-6-phosphate transaminase 2 mm
YEAST_2:1778809_AT Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
16051 carbohydrate biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4360 glutamine-fructose-6-phosphate transaminase (isomerizing) activity inferred from electronic annotation QuickGO AmiGO
5529 sugar binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92735 Hypothetical protein F22B3.4 [Caenorhabditis elegans] ref|NP_502156.1| F22B3.4 [Caenorhabditis elegans] 0.0
blast CAE59466 Hypothetical protein CBG02850 [Caenorhabditis briggsae] 0.0
blast CAC42276 Hypothetical protein F07A11.2b [Caenorhabditis elegans] ref|NP_496479.1| F07A11.2b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000583 EMBL-EBI Glutamine amidotransferase, class-II 1.6E-55
Pfam IPR001347 EMBL-EBI Sugar isomerase (SIS) 7.0E-43
Pfam IPR001347 EMBL-EBI Sugar isomerase (SIS) 1.3E-29
Sequence
Target Sequence 
>C. ELEGANS:193698_AT
gcctacacctcgcagattgtatcacttctgttgttcgcactcaccatctcggaagatcgc
atctcgaagatgaagagacgtgcagagattattgatgctctcaataatcttccaattctg
atccgcgatgttctcgatctggacgatcaggtactgaagatcgctgagcaaatctacaaa
gataagtcacttctcattatgggacgaggattgaactttgccacgtgtctcgaaggagct
ctcaagatcaaagagctctcgtacatgcattgtgaaggaatcatgtctggtgaactgaag
catgggcctcttgcaatggtcgatgaattcctcagcatctgcatggtagtatgcaacgat
catgtctacaagaagtcgttgaatgctcttcagcaagttgttgcgcgaaagggagccccg
atcattattgccgatagcagcgttccggaaagtgatctcgctggaatgaagcatgttctc
cgtgtcccaagaacagtcgactgtgttcaaaacatcctcacggtcattcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCTACACCTCGCAGATTGTATCAC 92 283 1525 Antisense
ATTGTATCACTTCTGTTGTTCGCAC 124 5 1540 Antisense
CACTCACCATCTCGGAAGATCGCAT 494 197 1562 Antisense
TTGCCACGTGTCTCGAAGGAGCTCT 30 699 1742 Antisense
AAAGAGCTCTCGTACATGCATTGTG 198 123 1774 Antisense
CTGAAGCATGGGCCTCTTGCAATGG 172 237 1819 Antisense
TGTTGCGCGAAAGGGAGCCCCGATC 699 561 1923 Antisense
GCCCCGATCATTATTGCCGATAGCA 695 285 1939 Antisense
AGCAGCGTTCCGGAAAGTGATCTCG 220 75 1960 Antisense
TCTCCGTGTCCCAAGAACAGTCGAC 392 609 2001 Antisense
TCAAAACATCCTCACGGTCATTCCT 422 629 2031 Antisense
Sequence Source Affymetrix Proprietary Database