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[193694_at]

Description W09D6.1 /REP_DB=WormBase Gene ID /WP=CE19015 /TR=Q9XUJ4 /GB=CAB04944.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=AMP-binding enzyme, PQQ enzyme repeat (3 domains)
Cluster 6grp_total > 78      4grp_total > 63      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 22.2
(-1.2)
A 		14
(-2)
A 		61.4
(3)
A 		4.9
(-3)
A 		57
B 4.2
(-1.7)
A 		4.7
(-1.1)
A 		7.9
(3)
A 		3.2
(-3)
A 		5
C 41.3
(3)
A 		7.2
(-3)
A 		28.4
(0.7)
A 		12
(-2.2)
A 		34
avr 22.6
(0.7) 		8.6
(-2.6) 		32.6
(3) 		6.7
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 33.1
(-1.6)
A 		15
(-2.5)
A 		24.5
(-2)
A 		5.4
(-3)
A 		31.9
(-1.6)
A 		121.9
(3)
A 		117
B 4.3
(-3)
A 		27.2
(-1)
A 		8.2
(-2.7)
A 		43.5
(0.5)
A 		30
(-0.7)
A 		71.9
(3)
A 		68
C 51.4
(0.5)
A 		16.1
(-3)
A 		32.4
(-1.4)
A 		33.4
(-1.3)
A 		76.2
(3)
A 		40.9
(-0.5)
A 		60
avr 29.6
(-2) 		19.4
(-3) 		21.7
(-2.8) 		27.4
(-2.2) 		46
(-0.3) 		78.2
(3) 		59
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 8.1
(-0.1)
A 		3
(-2.1)
A 		16
(3)
A 		0.7
(-3)
A 		15
B 1.3
(0.3)
A 		1.2
(-0.3)
A 		1.8
(3)
A 		0.7
(-3)
A 		1
C 13.7
(3)
A 		2.1
(-2.9)
A 		9.9
(1.1)
A 		2
(-3)
A 		12
avr 7.7
(1.9) 		2.1
(-2.3) 		9.2
(3) 		1.1
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 6.1
(-2)
A 		5.3
(-2.2)
A 		5.3
(-2.2)
A 		1.4
(-3)
A 		9.6
(-1.3)
A 		29.7
(3)
A 		28
B 1.5
(-3)
A 		9.1
(-1.2)
A 		2
(-2.9)
A 		11.5
(-0.6)
A 		12.2
(-0.5)
A 		26.8
(3)
A 		25
C 19.1
(1.3)
A 		4.9
(-3)
A 		8.2
(-2)
A 		6.7
(-2.5)
A 		24.7
(3)
A 		13.7
(-0.3)
A 		20
avr 8.9
(-1.8) 		6.4
(-2.6) 		5.2
(-3) 		6.5
(-2.6) 		15.5
(0.4) 		23.4
(3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193694_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6218
Sequence Type Exemplar sequence
Representative Public ID W09D6.1 NCBI
Target Description W09D6.1 /REP_DB=WormBase Gene ID /WP=CE19015 /TR=Q9XUJ4 /GB=CAB04944.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=AMP-binding enzyme, PQQ enzyme repeat (3 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_181907, NM_067023
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000027415 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11068769:11072841:1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_181907 6/11 Cross Hyb Matching Probes None
NM_067023 6/11 Cross Hyb Matching Probes None
SNAP00000027408 6/11 Cross Hyb Matching Probes None
W09D6.1a 6/11 Cross Hyb Matching Probes None
W09D6.1b 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11068730-11072837(+) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04944 Hypothetical protein W09D6.1a [Caenorhabditis elegans] ref|NP_499424.2| W09D6.1a [Caenorhabditis elegans] 0.0
blast CAD54160 Hypothetical protein W09D6.1b [Caenorhabditis elegans] ref|NP_871636.1| W09D6.1b [Caenorhabditis elegans] 0.0
blast CAE67618 Hypothetical protein CBG13167 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 2.0E-16
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 8.0E-33
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 0.0022
Sequence
Target Sequence 
>C. ELEGANS:193694_AT
tgaaccattgtttgtcggcagctcagtgattatcacgacagtgcacggagttttgaccag
ttttgatgtggagagtgggcggagacaggtacgaccacgcccagccaataagggggcggg
gctagtcacatccgcaatttggatggagcctagtgagacacgcccattatgtatgtactt
ttctgaccaaatctccctcgaaaatgaaaattgtttctgtgctccgttaccgattgccaa
ctcccacctcctcgtggccacccagtcaggaactgtgctccacgtgtcgctgagccccaa
attatcgattttgagcatttttcgatttgatctaccgtcacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAACCATTGTTTGTCGGCAGCTCA 147 575 2043 Antisense
GACAGTGCACGGAGTTTTGACCAGT 194 365 2079 Antisense
GGCGGAGACAGGTACGACCACGCCC 435 541 2120 Antisense
GCGGGGCTAGTCACATCCGCAATTT 247 287 2158 Antisense
GATGGAGCCTAGTGAGACACGCCCA 210 407 2184 Antisense
GACACGCCCATTATGTATGTACTTT 563 367 2199 Antisense
ATGTACTTTTCTGACCAAATCTCCC 269 41 2215 Antisense
GAAAATTGTTTCTGTGCTCCGTTAC 369 353 2248 Antisense
CAGTCAGGAACTGTGCTCCACGTGT 557 205 2305 Antisense
GTCGCTGAGCCCCAAATTATCGATT 642 473 2328 Antisense
TTTTTCGATTTGATCTACCGTCACC 205 673 2360 Antisense
Sequence Source Affymetrix Proprietary Database