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[193676_s_at]

Description F33D4.2A /REP_DB=WormBase Gene ID /WP=CE28014 /GEN=itr-1 /TR=O61193 /GB=AAB88374.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=inositol 1,4,5-triphosphate receptor
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 20      4grp_highest_8_8 > 52      4grp_high_8_8 > 25      6grp_highest_nonorm > 51      6grp_total > 40      4grp_total > 39      6grp_highest_sub > De      6grp_cgc > 71      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 408
1 2 3 4 range
A 556
(3)
P 		224.6
(-2.5)
P 		195.4
(-3)
P 		320.5
(-0.9)
P 		361
B 443.5
(3)
P 		231.6
(-1.3)
P 		148.5
(-3)
P 		317.5
(0.4)
P 		295
C 496.5
(3)
P 		265.4
(-2.9)
P 		260.9
(-3)
P 		281.3
(-2.5)
P 		236
avr 498.7
(3) 		240.5
(-2.2) 		201.6
(-3) 		306.4
(-0.9) 		297

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 459
1 2 3 4 5 6 range
A 590.6
(2.9)
P 		505.5
(1)
P 		593.8
(3)
P 		491
(0.7)
P 		427.9
(-0.8)
P 		329.9
(-3)
P 		264
B 683
(2.8)
P 		582.7
(0.5)
P 		690
(3)
P 		624.4
(1.5)
P 		431.3
(-3)
P 		515.9
(-1)
P 		259
C 511.7
(-0)
P 		742.2
(3)
P 		497.4
(-0.2)
P 		662.6
(2)
P 		283.1
(-3)
P 		473.4
(-0.5)
P 		459
avr 595.1
(2.6) 		610.1
(3) 		593.7
(2.6) 		592.7
(2.5) 		380.8
(-3) 		439.7
(-1.5) 		229
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 202.5
(3)
P 		48.8
(-2.9)
P 		51.1
(-2.8)
P 		46.9
(-3)
P 		156
B 141.4
(3)
P 		57.2
(-1.7)
P 		34.4
(-3)
P 		67.7
(-1.1)
P 		107
C 164.3
(3)
P 		76.3
(-1.5)
P 		90.9
(-0.8)
P 		47.4
(-3)
P 		117
avr 169.4
(3) 		60.8
(-2.6) 		58.8
(-2.8) 		54
(-3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 108.9
(-1.3)
P 		179.8
(3)
P 		129.3
(-0)
P 		124.5
(-0.3)
P 		129.1
(-0.1)
P 		80.3
(-3)
P 		100
B 237.6
(3)
P 		194
(-0.6)
P 		167.6
(-2.8)
P 		165.4
(-3)
P 		175.2
(-2.2)
P 		192.4
(-0.8)
P 		72
C 189.7
(1.4)
P 		226.5
(3)
P 		126.6
(-1.4)
P 		133.2
(-1.1)
P 		91.6
(-3)
P 		158.5
(-0)
P 		135
avr 178.7
(1.1) 		200.1
(3) 		141.2
(-2.2) 		141
(-2.2) 		132
(-3) 		143.7
(-2) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193676_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8987
Sequence Type Exemplar sequence
Representative Public ID F33D4.2A NCBI
Target Description F33D4.2A /REP_DB=WormBase Gene ID /WP=CE28014 /GEN=itr-1 /TR=O61193 /GB=AAB88374.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=inositol 1,4,5-triphosphate receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027999(11), NM_001028000(11), NM_001028002(11), NM_001028001(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027999 NCBI Caenorhabditis elegans Inositol Triphosphate Receptor family member (itr-1) (itr-1) mRNA, complete cds. 11/11 None
NM_001028000 NCBI Caenorhabditis elegans Inositol Triphosphate Receptor family member (itr-1) (itr-1) mRNA, complete cds. 11/11 None
NM_001028002 NCBI Caenorhabditis elegans Inositol Triphosphate Receptor family member (itr-1) (itr-1) mRNA, complete cds. 11/11 None
NM_001028001 NCBI Caenorhabditis elegans Inositol Triphosphate Receptor family member (itr-1) (itr-1) mRNA, complete cds. 11/11 None
SNAP00000032720 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7685641:7696687:1 11/11 None
GENEFINDER00000032735 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7693047:7696687:1 11/11 None
F33D4.2a ENSEMBL cdna:known chromosome:CEL140:IV:7675157:7696907:1 gene:F33D4.2 11/11 A 
F33D4.2d ENSEMBL cdna:known chromosome:CEL140:IV:7677552:7696687:1 gene:F33D4.2 11/11 A 
F33D4.2f ENSEMBL cdna:known chromosome:CEL140:IV:7681343:7696907:1 gene:F33D4.2 11/11 A 
F33D4.2e ENSEMBL cdna:known chromosome:CEL140:IV:7681343:7696907:1 gene:F33D4.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177546 5 4 NM_001027999 193676_s_at (11), 193688_s_at (11)
NM_001028000 193676_s_at (11), 193688_s_at (11)
NM_001028001 193676_s_at (11), 193688_s_at (11)
NM_001028002 193676_s_at (11), 193688_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7681699-7696693(+) 100.0 100.0
Public Domain and Genome References
Ensembl F33D4.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAW30668 Inositol triphosphate receptor protein 1, isoform a [Caenorhabditis elegans] emb|CAB45861.1| inositol 1,4,5-trisphosphate receptor [Caenorhabditis elegans] ref|NP_001023170.1| Inositol Triphosphate Receptor family member (itr-1) [Caenorhabditis elegans] 0.0
blast AAW30669 Inositol triphosphate receptor protein 1, isoform d [Caenorhabditis elegans] emb|CAB45862.1| inositol 1,4,5-trisphosphate receptor [Caenorhabditis elegans] ref|NP_001023171.1| Inositol Triphosphate Receptor family member (itr-1) [Caenorhabditis elegans] 0.0
blast AAK68366 Inositol triphosphate receptor protein 1, isoform f [Caenorhabditis elegans] emb|CAB45860.1| inositol 1,4,5-trisphosphate receptor [Caenorhabditis elegans] ref|NP_001023173.1| Inositol Triphosphate Receptor family member (itr-1) [Caenorhabditis elegans] gb|AAF05302.1| inositol 1,4,5-trisphosphate receptor [Caenorhabditis elegans] 0.0
blast AAK68365 Inositol triphosphate receptor protein 1, isoform e [Caenorhabditis elegans] emb|CAB45863.1| inositol 1,4,5-trisphosphate receptor [Caenorhabditis elegans] ref|NP_001023172.1| Inositol Triphosphate Receptor family member (itr-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 2.6E-12
Pfam IPR003608 EMBL-EBI MIR domain 6.7E-6
Pfam IPR003608 EMBL-EBI MIR domain 5.4E-15
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 9.5E-53
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 3.0E-22
Pfam IPR005821 EMBL-EBI Ion transport protein 2.6E-12
Pfam IPR003608 EMBL-EBI MIR domain 6.7E-6
Pfam IPR003608 EMBL-EBI MIR domain 5.4E-15
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 9.5E-53
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 3.0E-22
Pfam IPR005821 EMBL-EBI Ion transport protein 2.6E-12
Pfam IPR003608 EMBL-EBI MIR domain 6.7E-6
Pfam IPR003608 EMBL-EBI MIR domain 5.4E-15
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 9.5E-53
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 3.0E-22
Pfam IPR005821 EMBL-EBI Ion transport protein 2.6E-12
Pfam IPR003608 EMBL-EBI MIR domain 3.5E-12
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 9.5E-53
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 3.0E-22
Pfam IPR005821 EMBL-EBI Ion transport protein 2.6E-12
Pfam IPR003608 EMBL-EBI MIR domain 5.4E-15
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 9.5E-53
Pfam IPR000699 EMBL-EBI Intracellular calcium-release channel 3.0E-22
Pfam IPR005821 EMBL-EBI Ion transport protein 2.6E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023170.1 5 2475-2497,2510-2532,2571-2593,2616-2638,2731-2753
NP_001023171.1 5 2419-2441,2454-2476,2515-2537,2560-2582,2675-2697
NP_001023173.1 5 2429-2451,2464-2486,2525-2547,2570-2592,2685-2707
NP_001023172.1 5 2440-2462,2475-2497,2536-2558,2581-2603,2696-2718
F33D4.2a 5 2475-2497,2510-2532,2571-2593,2616-2638,2731-2753
F33D4.2d 5 2419-2441,2454-2476,2515-2537,2560-2582,2675-2697
F33D4.2f 5 2429-2451,2464-2486,2525-2547,2570-2592,2685-2707
F33D4.2e 5 2440-2462,2475-2497,2536-2558,2581-2603,2696-2718
SNAP00000032720 5 2143-2165,2178-2200,2239-2261,2284-2306,2399-2421
GENEFINDER00000032735 5 516-538,551-573,612-634,657-679,772-794
Sequence
Target Sequence 
>C. ELEGANS:193676_S_AT
gatatcaaggacttcgtaacggtggaggaattggtgatgtactcaggaatccagctccat
gggaagatatgttcatatggagagttgcttatgatatgactttctttgttgttcttatcg
ttattgttctcaatttgattttcggagtcatcatcgacacattcggagatttgagagccg
agaaaaacgagaaagaacaaattttgaaaaacaactgtttcatttgtggattagatagat
ctcgttttgacaaccgatcagtgacttttgaaacacatcgagaaacggagcacaatattt
ggcattatttgtattacattgtgatgctccaaattaaggatgaaactgagttcacaggac
ccgaaagctacgtggcacaatgtgttaaagatcggaatttggactggttcccaagaatgc
aggcattgtcgttacaagattcagaattagacacagatcaatcggaagttaaacaaatga
aggatcagttgttgcagatgatgacccttatgcgagagatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATCAAGGACTTCGTAACGGTGG 554 425 7883 Antisense
GATGTACTCAGGAATCCAGCTCCAT 481 409 7918 Antisense
ATGATATGACTTTCTTTGTTGTTCT 211 47 7973 Antisense
TGTTGTTCTTATCGTTATTGTTCTC 308 563 7989 Antisense
GATTTTCGGAGTCATCATCGACACA 603 429 8019 Antisense
ATCTCGTTTTGACAACCGATCAGTG 593 21 8121 Antisense
GAAACTGAGTTCACAGGACCCGAAA 73 357 8224 Antisense
GGACCCGAAAGCTACGTGGCACAAT 81 523 8239 Antisense
GGACTGGTTCCCAAGAATGCAGGCA 628 523 8283 Antisense
CAGTTGTTGCAGATGATGACCCTTA 463 205 8368 Antisense
GATGATGACCCTTATGCGAGAGATC 647 409 8379 Antisense
Sequence Source Affymetrix Proprietary Database