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[193668_s_at]

Description C12D12.2 /REP_DB=WormBase Gene ID /WP=CE06814 /GEN=glt-2 /TR=SW:Q10901 /GB=AAA96079.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=excitatory amino acid transporter
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 03      6grp_total > 05      4grp_total > 52      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3791
1 2 3 4 range
A 4757.8
(3)
P 		4124.3
(1.9)
P 		3915.9
(1.6)
P 		1185.6
(-3)
P 		3572
B 4890.6
(3)
P 		4293.9
(2)
P 		3847
(1.3)
P 		1283.6
(-3)
P 		3607
C 4203
(2.7)
P 		4364.1
(3)
P 		3692
(1.8)
P 		1099.4
(-3)
P 		3265
avr 4617.1
(3) 		4260.8
(2.4) 		3818.3
(1.6) 		1189.5
(-3) 		3428

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3287
1 2 3 4 5 6 range
A 4436.8
(3)
P 		3635.8
(1.4)
P 		2461.7
(-1)
P 		1756.7
(-2.4)
P 		2352.5
(-1.2)
P 		1445.6
(-3)
P 		2991
B 4393.9
(3)
P 		3322.8
(0.8)
P 		2395.1
(-1.1)
P 		2382.7
(-1.2)
P 		1841.1
(-2.3)
P 		1496.2
(-3)
P 		2898
C 4304.2
(3)
P 		3391.8
(1.3)
P 		2930.9
(0.4)
P 		1935.8
(-1.5)
P 		2045.9
(-1.3)
P 		1149.9
(-3)
P 		3154
avr 4378.3
(3) 		3450.1
(1.2) 		2595.9
(-0.5) 		2025.1
(-1.7) 		2079.8
(-1.6) 		1363.9
(-3) 		3014
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1559
1 2 3 4 range
A 1732.3
(3)
P 		895.3
(-0.2)
P 		1023.7
(0.3)
P 		173.5
(-3)
P 		1559
B 1559.6
(3)
P 		1060.5
(0.7)
P 		891.4
(-0.1)
P 		273.5
(-3)
P 		1286
C 1391.3
(3)
P 		1254.7
(2.3)
P 		1286.8
(2.5)
P 		185.4
(-3)
P 		1206
avr 1561.1
(3) 		1070.2
(0.8) 		1067.3
(0.8) 		210.8
(-3) 		1350

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1243
1 2 3 4 5 6 range
A 817.9
(-0)
P 		1293.1
(3)
P 		536.1
(-1.8)
P 		445.5
(-2.4)
P 		709.9
(-0.7)
P 		352
(-3)
P 		941
B 1528.9
(3)
P 		1106
(0.4)
P 		581.6
(-2.9)
P 		631.1
(-2.5)
P 		748
(-1.8)
P 		557.9
(-3)
P 		971
C 1595.4
(3)
P 		1034.9
(0.2)
P 		746
(-1.2)
P 		389.3
(-3)
P 		662
(-1.6)
P 		384.9
(-3)
P 		1211
avr 1314.1
(3) 		1144.7
(1.8) 		621.2
(-1.7) 		488.6
(-2.6) 		706.6
(-1.1) 		431.6
(-3) 		883
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193668_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18387
Sequence Type Exemplar sequence
Representative Public ID C12D12.2 NCBI
Target Description C12D12.2 /REP_DB=WormBase Gene ID /WP=CE06814 /GEN=glt-2 /TR=SW:Q10901 /GB=AAA96079.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=excitatory amino acid transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029222(11), NM_001029223(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029222 NCBI Caenorhabditis elegans GLutamate Transporter family member (glt-1) (glt-1) mRNA, complete cds. 11/11 None
NM_001029223 NCBI Caenorhabditis elegans GLutamate Transporter family member (glt-1) (glt-1) mRNA, complete cds. 11/11 None
SNAP00000040496 ENSEMBL cdna:SNAP chromosome:CEL140:X:3496611:3498874:1 11/11 None
GENEFINDER00000040501 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3496611:3498874:1 11/11 None
C12D12.2a.2 ENSEMBL cdna:known chromosome:CEL140:X:3495915:3498874:1 gene:C12D12.2 11/11 None
C12D12.2a.1 ENSEMBL cdna:known chromosome:CEL140:X:3496013:3499156:1 gene:C12D12.2 11/11 None
C12D12.2b ENSEMBL cdna:known chromosome:CEL140:X:3496611:3498874:1 gene:C12D12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3496077-3498767(+) 100.0 100.0
Public Domain and Genome References
Ensembl C12D12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL00857 Glutamate transporter family protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024393.1| GLutamate Transporter family member (glt-1) [Caenorhabditis elegans] sp|Q10901|EAA1_CAEEL Excitatory amino acid transporter (Sodium-dependent glutamate/ aspartate transporter) dbj|BAA21840.1| glutamate transporter [Caenorhabditis elegans] dbj|BAA13164.1| glutamate transporter [Caenorhabditis elegans] 0.0
blast AAB41909 CeGlt-2 0.0
blast AAL00858 Glutamate transporter family protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024394.1| GLutamate Transporter family member (glt-1) [Caenorhabditis elegans] 0.0
blast AAB41910 CeGlt-1 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001991 EMBL-EBI Sodium:dicarboxylate symporter 1.0E-126
Pfam IPR001991 EMBL-EBI Sodium:dicarboxylate symporter 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024393.1 9 21-43,58-80,93-115,200-222,243-264,279-301,313-335,364-386,398-420
NP_001024394.1 9 9-31,46-68,81-103,189-211,232-253,268-290,302-324,353-375,387-409
C12D12.2a.2 9 21-43,58-80,93-115,200-222,243-264,279-301,313-335,364-386,398-420
C12D12.2a.1 9 21-43,58-80,93-115,200-222,243-264,279-301,313-335,364-386,398-420
C12D12.2b 9 9-31,46-68,81-103,189-211,232-253,268-290,302-324,353-375,387-409
SNAP00000040496 9 9-31,46-68,81-103,189-211,232-253,268-290,302-324,353-375,387-409
GENEFINDER00000040501 9 9-31,46-68,81-103,189-211,232-253,268-290,302-324,353-375,387-409
Sequence
Target Sequence 
>C. ELEGANS:193668_S_AT
tacatggtaaccgttctctcaggacttgctattcactcattgatttctcttccactcatc
ttcttcgttactaccaagaagaacccatatgtgttcatgagaggactcttccaagcctgg
attacggccctcggaactgcttccagctctgccaccctaccaatcactttcaactgcctc
gaagagaatttgggtgtggaccgtcgtgtcacacgttttgtccttcctgtcggagctacc
atcaacatggatggaactgcattgtacgaagctgtggctgctattttcattgcccaaatt
aacggagttcacctttctttcggacaggtcgtcactgtcagtttgaccgccaccttggcc
tcaatcggagctgcttcggtaccatctgccggacttgtcactatgctcttagttcttact
gctgtcggacttccagtcaaggatgtttcccttatcgttgccgtcgattggcttcttgac
agaatcagaacttccatcaatgtgctcggagatgccatgggcgccggaattgtctaccac
tactctaaagccgatcttgatgcacatgaccgtcttgccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACATGGTAACCGTTCTCTCAGGAC 177 645 952 Antisense
CTCAGGACTTGCTATTCACTCATTG 515 229 969 Antisense
GACTCTTCCAAGCCTGGATTACGGC 445 371 1055 Antisense
ATCACTTTCAACTGCCTCGAAGAGA 338 21 1114 Antisense
GACCGTCGTGTCACACGTTTTGTCC 550 381 1150 Antisense
GGTCGTCACTGTCAGTTTGACCGCC 697 499 1278 Antisense
TATGCTCTTAGTTCTTACTGCTGTC 41 657 1353 Antisense
TTACTGCTGTCGGACTTCCAGTCAA 442 679 1367 Antisense
TGCCGTCGATTGGCTTCTTGACAGA 29 587 1410 Antisense
CTACCACTACTCTAAAGCCGATCTT 547 225 1485 Antisense
CTTGATGCACATGACCGTCTTGCCG 534 213 1507 Antisense
Sequence Source Affymetrix Proprietary Database