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[193659_at] NAD-dependent glycerol-3-phosphate dehydrogenase

Description F47G4.3 /REP_DB=WormBase Gene ID /WP=CE18701 /TR=Q9XTS4 /GB=CAB16310.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NAD-dependent glycerol-3-phosphate dehydrogenase
Cluster 6grp_total > 94      4grp_total > 02      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6990
1 2 3 4 range
A 57
(-3)
A 		150.6
(-2.9)
P 		388.2
(-2.7)
P 		6634.8
(3)
P 		6578
B 30.1
(-3)
A 		177.3
(-2.9)
P 		359.3
(-2.7)
P 		7003.3
(3)
P 		6973
C 68.3
(-3)
A 		172.9
(-2.9)
A 		319.8
(-2.8)
P 		7020
(3)
P 		6952
avr 51.8
(-3) 		166.9
(-2.9) 		355.8
(-2.7) 		6886
(3) 		6834

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1285
1 2 3 4 5 6 range
A 97.1
(-3)
A 		267.1
(-2.2)
P 		526
(-1)
P 		688.2
(-0.2)
P 		1377.7
(3)
P 		1040.8
(1.4)
P 		1281
B 121.2
(-3)
A 		263.2
(-2.2)
P 		500.6
(-1)
P 		575
(-0.6)
P 		1248.3
(3)
P 		952.4
(1.4)
P 		1127
C 92.3
(-3)
A 		269.9
(-2.1)
P 		544.9
(-0.7)
P 		511.2
(-0.8)
P 		1252
(3)
P 		844.2
(0.9)
P 		1160
avr 103.5
(-3) 		266.7
(-2.2) 		523.8
(-0.9) 		591.5
(-0.5) 		1292.7
(3) 		945.8
(1.2) 		1189
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1483
1 2 3 4 range
A 20.8
(-3)
A 		32.7
(-2.9)
P 		101.5
(-2.5)
P 		971.2
(3)
P 		950
B 9.6
(-3)
A 		43.8
(-2.9)
P 		83.3
(-2.7)
P 		1492.4
(3)
P 		1483
C 22.6
(-3)
A 		49.7
(-2.9)
A 		111.5
(-2.5)
P 		1183.7
(3)
P 		1161
avr 17.7
(-3) 		42.1
(-2.9) 		98.8
(-2.6) 		1215.8
(3) 		1198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 489
1 2 3 4 5 6 range
A 17.9
(-3)
A 		95
(-1.8)
P 		114.6
(-1.5)
P 		174.6
(-0.6)
P 		415.7
(3)
P 		253.4
(0.6)
P 		398
B 42.2
(-3)
A 		87.6
(-2.4)
P 		121.6
(-2)
P 		152.3
(-1.6)
P 		507.2
(3)
P 		355.2
(1)
P 		465
C 34.2
(-3)
A 		82.3
(-2.2)
P 		138.7
(-1.3)
P 		102.8
(-1.9)
P 		405.1
(3)
P 		282.6
(1)
P 		371
avr 31.4
(-3) 		88.3
(-2.2) 		125
(-1.6) 		143.2
(-1.4) 		442.7
(3) 		297.1
(0.9) 		411
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193659_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.433
Sequence Type Exemplar sequence
Representative Public ID F47G4.3 NCBI
Target Description F47G4.3 /REP_DB=WormBase Gene ID /WP=CE18701 /TR=Q9XTS4 /GB=CAB16310.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NAD-dependent glycerol-3-phosphate dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061053(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061053 NCBI Caenorhabditis elegans F47G4.3 (F47G4.3) mRNA, complete cds. 11/11 None
SNAP00000012295 ENSEMBL cdna:SNAP chromosome:CEL140:I:14083995:14089054:1 11/11 None
GENEFINDER00000012299 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14083995:14086186:1 11/11 None
F47G4.3 ENSEMBL cdna:known chromosome:CEL140:I:14083954:14086323:1 gene:F47G4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14083925-14086117(+) 100.0 100.0
Public Domain and Genome References
Gene Title NAD-dependent glycerol-3-phosphate dehydrogenase
Ensembl F47G4.3
Entrez Gene 173272 Entrez gene
SwissProt Q9XTS4 EMBL-EBI
WormBase CE18701 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
6072 glycerol-3-phosphate metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
9331 glycerol-3-phosphate dehydrogenase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4367 glycerol-3-phosphate dehydrogenase (NAD+) activity inferred from electronic annotation QuickGO AmiGO
16614 oxidoreductase activity, acting on CH-OH group of donors inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB16310 Hypothetical protein F47G4.3 [Caenorhabditis elegans] ref|NP_493454.1| F47G4.3 [Caenorhabditis elegans] 0.0
blast CAE71830 Hypothetical protein CBG18871 [Caenorhabditis briggsae] 0.0
blast CAA80176 Hypothetical protein K11H3.1a [Caenorhabditis elegans] ref|NP_499188.2| K11H3.1a [Caenorhabditis elegans] 1.0E-107
Protein Domains
Database ID Description E-Value
Pfam IPR006168 EMBL-EBI NAD-dependent glycerol-3-phosphate dehydrogenase 1.0E-126
Pfam IPR006168 EMBL-EBI NAD-dependent glycerol-3-phosphate dehydrogenase 8.0E-78
Pfam IPR006168 EMBL-EBI NAD-dependent glycerol-3-phosphate dehydrogenase 5.8E-52
Sequence
Target Sequence 
>C. ELEGANS:193659_AT
atgaggccgtggagctttgcggagcactaaagaacattgtcgcctgcgccgcgggatttg
ccgatgggcttggctgggcctacaacgtgaaatccgctatcatccgtctgggactcctag
aaaccaagaagtttgtggagcacttctacccaagttccgtcggacatacatacttcgagt
cgtgtggagtggctgatctcattaccacttgctatggaggtcgcaatcgaaaagttgcgg
aggcattcatcaagagtgataagccgctccgagtcattgagcaggaacttctcaagggtc
aaagtgctcagggcccgccaacagctcaggatgtttatgaaatgctggagatcaatgaaa
tctcggaaaagttcccaatcttcgcgtcagtgcataaggtgttcaccgggcaccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAGGCCGTGGAGCTTTGCGGAGC 487 45 671 Antisense
ACTAAAGAACATTGTCGCCTGCGCC 416 101 696 Antisense
GATTTGCCGATGGGCTTGGCTGGGC 426 427 725 Antisense
GCTGGGCCTACAACGTGAAATCCGC 585 293 743 Antisense
ATCATCCGTCTGGGACTCCTAGAAA 45 29 769 Antisense
AGTTTGTGGAGCACTTCTACCCAAG 476 63 800 Antisense
TTCTACCCAAGTTCCGTCGGACATA 361 673 814 Antisense
GTGGCTGATCTCATTACCACTTGCT 349 491 859 Antisense
TGATAAGCCGCTCCGAGTCATTGAG 404 563 927 Antisense
TCGGAAAAGTTCCCAATCTTCGCGT 403 605 1033 Antisense
GCATAAGGTGTTCACCGGGCACCAC 349 295 1062 Antisense
Sequence Source Affymetrix Proprietary Database