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[193657_at] guanine nucleotide-binding protein

Description C16A11.1 /REP_DB=WormBase Gene ID /WP=CE20515 /GEN=gpa-11 /TR=O76584 /GB=AAK31414.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_total > 25      4grp_total > 22      6grp_cgc > 33      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 76.6
(2.3)
P 		14.6
(-2.1)
A 		2.6
(-3)
A 		86.2
(3)
P 		84
B 99
(3)
P 		20
(-2.3)
A 		9.5
(-3)
A 		80.1
(1.7)
A 		90
C 93.9
(2.4)
P 		48.1
(-0.6)
A 		12.3
(-3)
A 		103.4
(3)
A 		91
avr 89.8
(3) 		27.6
(-1.6) 		8.1
(-3) 		89.9
(3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 91.9
(2.4)
P 		100.9
(3)
P 		55.3
(0.1)
A 		7.9
(-3)
A 		41.1
(-0.9)
A 		9.5
(-2.9)
A 		93
B 61.8
(0.4)
P 		86
(3)
P 		64.7
(0.7)
A 		37.3
(-2.2)
A 		29.7
(-3)
A 		37.7
(-2.1)
A 		56
C 64.7
(2.3)
P 		70.5
(3)
M 		18.9
(-3)
A 		28.9
(-1.8)
A 		22.1
(-2.6)
A 		39.8
(-0.6)
A 		52
avr 72.8
(1.7) 		85.8
(3) 		46.3
(-0.9) 		24.7
(-3) 		31
(-2.4) 		29
(-2.6) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 27.9
(3)
P 		3.2
(-2.4)
A 		0.7
(-3)
A 		12.6
(-0.4)
P 		27
B 31.6
(3)
P 		5
(-2.4)
A 		2.2
(-3)
A 		17.1
(0)
A 		29
C 31.1
(3)
P 		13.8
(-0.9)
A 		4.3
(-3)
A 		17.4
(-0.1)
A 		27
avr 30.2
(3) 		7.3
(-1.9) 		2.4
(-3) 		15.7
(-0.1) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 16.9
(-0.4)
P 		35.9
(3)
P 		12
(-1.2)
A 		2
(-3)
A 		12.4
(-1.2)
A 		2.3
(-2.9)
A 		34
B 21.5
(0.7)
P 		28.6
(3)
P 		15.7
(-1.1)
A 		9.9
(-3)
A 		12.1
(-2.3)
A 		14
(-1.7)
A 		19
C 24
(3)
P 		21.5
(2.2)
M 		4.8
(-3)
A 		5.8
(-2.7)
A 		7.2
(-2.3)
A 		13.3
(-0.3)
A 		19
avr 20.8
(0.9) 		28.7
(3) 		10.8
(-1.7) 		5.9
(-3) 		10.6
(-1.8) 		9.9
(-1.9) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193657_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4890
Sequence Type Exemplar sequence
Representative Public ID C16A11.1 NCBI
Target Description C16A11.1 /REP_DB=WormBase Gene ID /WP=CE20515 /GEN=gpa-11 /TR=O76584 /GB=AAK31414.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062347(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062347 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-11) (gpa-11) mRNA, complete cds. 11/11 None
SNAP00000042944 ENSEMBL cdna:SNAP chromosome:CEL140:II:4252707:4259421:1 11/11 None
GENEFINDER00000042953 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4247191:4255275:1 11/11 None
C16A11.1 ENSEMBL cdna:known chromosome:CEL140:II:4253046:4255275:1 gene:C16A11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4253044-4255274(+) 99.63 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-11
Ensembl C16A11.1
Entrez Gene 173759 Entrez gene
SwissProt O76584 EMBL-EBI
WormBase CE20515 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
7186 G-protein coupled receptor protein signaling pathway inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5834 heterotrimeric G-protein complex inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from sequence similarity QuickGO AmiGO
3924 GTPase activity inferred from sequence similarity QuickGO AmiGO
5057 receptor signaling protein activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31414 G protein, alpha subunit protein 11 [Caenorhabditis elegans] ref|NP_494748.1| G Protein, Alpha subunit family member (gpa-11) [Caenorhabditis elegans] gb|AAG32086.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|O76584|GPA11_CAEEL Guanine nucleotide-binding protein alpha-11 subunit 0.0
blast T33351 hypothetical protein C16A11.1 - Caenorhabditis elegans 0.0
blast AAW02895 gpa-11 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 5.1E-123
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.2E-122
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 6.3E-125
Sequence
Target Sequence 
>C. ELEGANS:193657_AT
atcagcacacgagtacaagccaacagagatggatgttctaagagcacgagttccgacgac
gggtattacggaaattgagttcccatttaaacaagcaagtttaagaatggtggatgtcgg
tggtcaacgatcagaacagaggaagtggatccactgtttcgacaatgtgaatggagtctt
atttatagcagcaataagtggatataatttatatgatgaagatgaagaaaatagaaaaga
tgatggaacaccaacaaaaaccaatcgattgcgatattcaatggaattattcaaaagaat
cgcgaatcatcagtgctttagcaagaaaaccgcgatgattctatttttaaacaaaattga
tattttcaaagaaaaaatcggaaagtatccactaacaacgtgctttaaaaattacaaagg
tgtgaatgcattcgagccggcgtgtaagtacgtgacagaccgattctcacggcttgtcag
cggggatattcaacacgagaagcctctgtacacgcatataaccaatgcgacagacactcg
gaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAGCACACGAGTACAAGCCAACA 39 29 489 Antisense
AGAGCACGAGTTCCGACGACGGGTA 82 63 529 Antisense
GGTGGATGTCGGTGGTCAACGATCA 36 499 597 Antisense
GAATCGCGAATCATCAGTGCTTTAG 569 329 785 Antisense
AAGTATCCACTAACAACGTGCTTTA 281 155 871 Antisense
TGAATGCATTCGAGCCGGCGTGTAA 592 573 911 Antisense
CGGCGTGTAAGTACGTGACAGACCG 445 225 926 Antisense
TGACAGACCGATTCTCACGGCTTGT 570 571 941 Antisense
TTCTCACGGCTTGTCAGCGGGGATA 644 689 952 Antisense
GAAGCCTCTGTACACGCATATAACC 237 345 987 Antisense
AACCAATGCGACAGACACTCGGAAT 219 149 1008 Antisense
Sequence Source Affymetrix Proprietary Database