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[193639_at] 1-acyl-SN-glycerol-3-phosphate acyltransferase

Description F59F4.4 /REP_DB=WormBase Gene ID /WP=CE11552 /TR=SW:Q93841 /GB=CAB03160.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=1-acyl-SN-glycerol-3-phosphate acyltransferase
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 04      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 41      6grp_total > 24      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 701
1 2 3 4 range
A 1039.3
(3)
P 		786.7
(0.7)
P 		844
(1.2)
P 		377.1
(-3)
P 		662
B 1075.3
(3)
P 		810.9
(0.5)
P 		913.1
(1.4)
P 		451.1
(-3)
P 		624
C 966.9
(2.9)
P 		973.5
(3)
P 		780.4
(1.1)
P 		374.4
(-3)
P 		599
avr 1027.2
(3) 		857
(1.4) 		845.8
(1.3) 		400.9
(-3) 		626

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 716
1 2 3 4 5 6 range
A 879.8
(3)
P 		853.9
(2.6)
P 		609.5
(-1.7)
P 		539
(-2.9)
P 		558.6
(-2.5)
P 		531.3
(-3)
P 		349
B 802.8
(3)
P 		708.8
(1.7)
P 		794.5
(2.9)
P 		583.9
(-0.1)
P 		561.3
(-0.4)
P 		378.4
(-3)
P 		424
C 1094.7
(3)
P 		687.5
(-0.5)
P 		805.8
(0.5)
P 		431.7
(-2.7)
P 		602.6
(-1.2)
P 		398.6
(-3)
P 		696
avr 925.8
(3) 		750.1
(0.8) 		736.6
(0.7) 		518.2
(-2) 		574.2
(-1.3) 		436.1
(-3) 		490
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 323
1 2 3 4 range
A 378.4
(3)
P 		170.8
(-0.9)
P 		220.6
(0.1)
P 		55.2
(-3)
P 		323
B 342.9
(3)
P 		200.3
(-0.5)
P 		211.6
(-0.2)
P 		96.1
(-3)
P 		247
C 320.1
(3)
P 		279.9
(2.1)
P 		272
(1.9)
P 		63.1
(-3)
P 		257
avr 347.1
(3) 		217
(0.2) 		234.7
(0.6) 		71.5
(-3) 		276

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 319
1 2 3 4 5 6 range
A 162.2
(-1.9)
P 		303.7
(3)
P 		132.7
(-2.9)
P 		136.7
(-2.7)
P 		168.6
(-1.7)
P 		129.4
(-3)
P 		174
B 279.3
(3)
P 		235.9
(1.1)
P 		192.9
(-0.8)
P 		154.6
(-2.4)
P 		228
(0.8)
P 		141.1
(-3)
P 		138
C 405.8
(3)
P 		209.8
(-0.7)
P 		205.1
(-0.8)
P 		86.8
(-3)
P 		195
(-1)
P 		133.4
(-2.1)
P 		319
avr 282.4
(3) 		249.8
(1.7) 		176.9
(-1) 		126
(-3) 		197.2
(-0.3) 		134.6
(-2.7) 		156
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193639_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17686
Sequence Type Exemplar sequence
Representative Public ID F59F4.4 NCBI
Target Description F59F4.4 /REP_DB=WormBase Gene ID /WP=CE11552 /TR=SW:Q93841 /GB=CAB03160.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=1-acyl-SN-glycerol-3-phosphate acyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078205(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078205 NCBI Caenorhabditis elegans ACyLtransferase-like family member (acl-1) (acl-1) mRNA, complete cds. 11/11 None
F59F4.4 ENSEMBL cdna:known chromosome:CEL140:X:15849817:15851642:1 gene:F59F4.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000031693 7/11 Cross Hyb Matching Probes None
SNAP00000031691 7/11 Cross Hyb Matching Probes None
GENEFINDER00000026516 4/11 Cross Hyb Matching Probes None
SNAP00000026500 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:15849816-15851484(+) 100.0 100.0
Public Domain and Genome References
Gene Title 1-acyl-SN-glycerol-3-phosphate acyltransferase
Gene Symbol acl-1
Ensembl F59F4.4
Entrez Gene 181671 Entrez gene
SwissProt Q93841 EMBL-EBI
WormBase CE11552 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
8654 phospholipid biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3841 1-acylglycerol-3-phosphate O-acyltransferase activity inferred from electronic annotation QuickGO AmiGO
8415 acyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03160 Hypothetical protein F59F4.4 [Caenorhabditis elegans] emb|CAB03139.1| Hypothetical protein F59F4.4 [Caenorhabditis elegans] ref|NP_510606.1| ACyLtransferase-like family member (acl-1) [Caenorhabditis elegans] sp|Q93841|PLC1_CAEEL Putative 1-acyl-sn-glycerol-3-phosphate acyltransferase F59F4.4 (1-AGP acyltransferase) (1-AGPAT) (Lysophosphatidic acid acyltransferase) (LPAAT) 1.0E-138
blast CAE69734 Hypothetical protein CBG16005 [Caenorhabditis briggsae] 1.0E-118
blast CAE61082 Hypothetical protein CBG04832 [Caenorhabditis briggsae] 1.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR002123 EMBL-EBI Phospholipid/glycerol acyltransferase 4.8E-46
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510606.1 2 4-26,28-50
F59F4.4 2 4-26,28-50
Sequence
Target Sequence 
>C. ELEGANS:193639_AT
tcattgccaaccatcagagtgctcttgacgtcttgggaatgtcattcgcctggccagtcg
attgtgtcgtgatgctcaagtcgagcctcaagtaccttcccggcttcaatctctgtgcat
atctttgtgattccgtgtacatcaaccgattcagcaaggaaaaggcgctcaagacagttg
acaccacgctccacgagattgtcaccaagaagagaaaggtgtggatctacccagaaggaa
cccgcaacgccgagccagagcttctgccgttcaagaaaggagccttcattcttgcgaaac
aagccaagatcccaatcgtcccatgcgtcttctcatctcacaagttcttctacagccacg
ccgagaaacgtctcacctcaggaaactgcatcattgacattcttccagaagtagattcat
cgaaattcgactcaattgatgacctctcagctcattgtaggaaaataatgcaggctcacc
gcgagaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATTGCCAACCATCAGAGTGCTCT 201 619 281 Antisense
TCAGAGTGCTCTTGACGTCTTGGGA 260 623 294 Antisense
CCTGGCCAGTCGATTGTGTCGTGAT 691 265 329 Antisense
TCAAGTCGAGCCTCAAGTACCTTCC 679 627 356 Antisense
GATTCCGTGTACATCAACCGATTCA 135 433 409 Antisense
GATCTACCCAGAAGGAACCCGCAAC 425 417 504 Antisense
GAAAGGAGCCTTCATTCTTGCGAAA 338 361 555 Antisense
GAAACGTCTCACCTCAGGAAACTGC 177 357 645 Antisense
GAAACTGCATCATTGACATTCTTCC 599 355 662 Antisense
GACTCAATTGATGACCTCTCAGCTC 500 373 709 Antisense
ATAATGCAGGCTCACCGCGAGAAGC 529 19 745 Antisense
Sequence Source Affymetrix Proprietary Database