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[193630_at] sodium/calcium exchanger protein like

Description ZC168.1 /REP_DB=WormBase Gene ID /WP=CE06567 /GEN=ncx-3 /TR=Q21895 /GB=CAA94363.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=sodium-calcium exchanger protein like
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 50      6grp_total > 07      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 902
1 2 3 4 range
A 868.3
(3)
P 		260.3
(-1.9)
P 		127.3
(-3)
P 		186.7
(-2.5)
P 		741
B 1029.3
(3)
P 		282.6
(-2.1)
P 		182.2
(-2.8)
P 		150.2
(-3)
P 		879
C 868.7
(3)
P 		268.5
(-2.5)
P 		211.8
(-3)
P 		302.6
(-2.2)
P 		657
avr 922.1
(3) 		270.5
(-2.2) 		173.8
(-3) 		213.2
(-2.7) 		748

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 818
1 2 3 4 5 6 range
A 1028
(3)
P 		570.3
(-0.5)
P 		421.2
(-1.7)
P 		458.9
(-1.4)
P 		245.6
(-3)
P 		285.5
(-2.7)
P 		782
B 904.3
(3)
P 		530
(-0.2)
P 		423.2
(-1.2)
P 		395.9
(-1.4)
P 		244.2
(-2.7)
P 		210.3
(-3)
P 		694
C 762.1
(3)
P 		686.2
(2.1)
P 		483.8
(-0.3)
P 		421.4
(-1.1)
P 		263.9
(-3)
P 		261.1
(-3)
P 		501
avr 898.1
(3) 		595.5
(0.2) 		442.7
(-1.2) 		425.4
(-1.4) 		251.2
(-3) 		252.3
(-3) 		647
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 316.2
(3)
P 		56.5
(-2.4)
P 		33.3
(-2.9)
P 		27.3
(-3)
P 		289
B 328.2
(3)
P 		69.8
(-2.2)
P 		42.2
(-2.8)
P 		32
(-3)
P 		296
C 287.6
(3)
P 		77.2
(-2.3)
P 		73.8
(-2.4)
P 		51
(-3)
P 		237
avr 310.7
(3) 		67.8
(-2.3) 		49.8
(-2.7) 		36.8
(-3) 		274

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 189.5
(2.4)
P 		202.8
(3)
P 		91.7
(-2)
P 		116.4
(-0.9)
P 		74.1
(-2.8)
P 		69.5
(-3)
P 		133
B 314.6
(3)
P 		176.4
(-0.5)
P 		102.8
(-2.4)
P 		104.9
(-2.3)
P 		99.2
(-2.5)
P 		78.4
(-3)
P 		236
C 282.5
(3)
P 		209.4
(0.8)
P 		123.1
(-1.8)
P 		84.7
(-3)
P 		85.4
(-3)
P 		87.4
(-2.9)
P 		198
avr 262.2
(3) 		196.2
(0.8) 		105.9
(-2.1) 		102
(-2.2) 		86.2
(-2.7) 		78.4
(-3) 		184
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193630_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9289
Sequence Type Exemplar sequence
Representative Public ID ZC168.1 NCBI
Target Description ZC168.1 /REP_DB=WormBase Gene ID /WP=CE06567 /GEN=ncx-3 /TR=Q21895 /GB=CAA94363.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=sodium-calcium exchanger protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069583(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069583 NCBI Caenorhabditis elegans Na/Ca eXchangers family member (ncx-3) (ncx-3) mRNA, complete cds. 11/11 None
SNAP00000040682 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10718831:10721491:1 11/11 None
GENEFINDER00000040692 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10718831:10721491:1 11/11 None
ZC168.1 ENSEMBL cdna:known chromosome:CEL140:IV:10716628:10721727:1 gene:ZC168.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10716633-10721497(+) 100.0 100.0
Public Domain and Genome References
Gene Title sodium/calcium exchanger protein like
Gene Symbol ncx-3
Ensembl ZC168.1
Entrez Gene 177963 Entrez gene
SwissProt Q21895 EMBL-EBI
WormBase CE06567 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6816 calcium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5432 calcium:sodium antiporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94387 Hypothetical protein ZC168.1 [Caenorhabditis elegans] emb|CAA94363.1| Hypothetical protein ZC168.1 [Caenorhabditis elegans] ref|NP_501984.1| Na/Ca eXchangers family member (ncx-3) [Caenorhabditis elegans] 0.0
blast CAE70856 Hypothetical protein CBG17641 [Caenorhabditis briggsae] 0.0
blast XP_794199 PREDICTED: similar to Na/Ca eXchanger (ncx-3), partial [Strongylocentrotus purpuratus] 1.0E-115
Protein Domains
Database ID Description E-Value
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 4.6E-29
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 1.5E-26
Pfam IPR003644 EMBL-EBI Na-Ca exchanger/integrin-beta4 1.8E-28
Pfam IPR003644 EMBL-EBI Na-Ca exchanger/integrin-beta4 2.3E-31
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 1.5E-26
Pfam IPR003644 EMBL-EBI Na-Ca exchanger/integrin-beta4 1.2E-25
Pfam IPR003644 EMBL-EBI Na-Ca exchanger/integrin-beta4 5.4E-27
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 1.5E-26
Pfam IPR003644 EMBL-EBI Na-Ca exchanger/integrin-beta4 2.3E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501984.1 8 25-47,126-148,160-182,192-209,630-652,708-727,740-760,780-799
ZC168.1 8 25-47,126-148,160-182,192-209,630-652,708-727,740-760,780-799
SNAP00000040682 5 299-321,323-345,377-396,409-429,449-468
GENEFINDER00000040692 5 285-307,309-331,363-382,395-415,435-454
Sequence
Target Sequence 
>C. ELEGANS:193630_AT
ttgtatacttcacgctcttgcattcccatggaaatttgcatttgctttcctaccaccacc
gacgattttctatggatacccgtgtttcgtagtagctctgattggaatcggtttggtgac
tgctgttgttggagatgttgcatcgatatttggatgtatggttggactgaaagatgctgt
aactgcaatcactctcgttgcacttggaacttctctaccagatacttttgcttcgaagat
tgctgcggaaagtgatgatactgctgacaacgctgtcggaaatgttaccggatcaaattc
agtaaatgtgttcctcggactcggccttccctgggtgatcgcatctctttattgggcatc
gaaaggagaatcattccgcgtggatgctggtgatctcggtttcagcgtcacagtgttcat
gatctgctcagtgctcttccttgttgttctcgttctacgacgaaagttaaaagcatttgg
acaaggagaattgggtggtccattcggtacaaaaacactgtctgcactgttcttcgtggg
cctctggattgtctacgttggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTATACTTCACGCTCTTGCATTC 380 707 1836 Antisense
GATACCCGTGTTTCGTAGTAGCTCT 238 423 1910 Antisense
GAATCGGTTTGGTGACTGCTGTTGT 503 329 1940 Antisense
TGCTGTAACTGCAATCACTCTCGTT 352 583 2010 Antisense
CGTTGCACTTGGAACTTCTCTACCA 176 247 2031 Antisense
GTGATCGCATCTCTTTATTGGGCAT 248 485 2170 Antisense
GAATCATTCCGCGTGGATGCTGGTG 99 327 2203 Antisense
TGGTGATCTCGGTTTCAGCGTCACA 490 553 2223 Antisense
GTGTTCATGATCTGCTCAGTGCTCT 66 485 2248 Antisense
AAAACACTGTCTGCACTGTTCTTCG 197 131 2347 Antisense
GGCCTCTGGATTGTCTACGTTGGAC 172 541 2374 Antisense
Sequence Source Affymetrix Proprietary Database