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[193625_at] transcriptional activator protein PUR-alpha like

Description F20D1.4 /REP_DB=WormBase Gene ID /WP=CE09497 /TR=Q93537 /GB=CAB01747.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=transcriptional activator protein PUR-alpha like
Cluster 6grp_total > 90      4grp_total > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 5.6
(-3)
A 		8.5
(-2.5)
A 		42.9
(3)
A 		12.4
(-1.9)
A 		37
B 5.8
(-2.2)
A 		9
(3)
A 		5.3
(-3)
A 		5.4
(-2.8)
A 		4
C 8.8
(3)
A 		8.2
(2.4)
A 		3
(-3)
A 		4.3
(-1.7)
A 		6
avr 6.7
(-3) 		8.6
(-1.9) 		17.1
(3) 		7.4
(-2.6) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 53
(0.7)
A 		10.2
(-2.5)
A 		4
(-3)
A 		84.1
(3)
A 		6.9
(-2.8)
A 		5.9
(-2.9)
A 		80
B 7.9
(-1.3)
A 		6.4
(-2.4)
A 		12.7
(2.1)
A 		14
(3)
A 		5.5
(-3)
A 		7.4
(-1.7)
A 		9
C 9.1
(-0.1)
A 		4
(-3)
A 		5.7
(-2)
A 		14.7
(3)
A 		6.6
(-1.5)
A 		4.9
(-2.5)
A 		11
avr 23.3
(0.3) 		6.9
(-2.8) 		7.5
(-2.7) 		37.6
(3) 		6.3
(-3) 		6.1
(-3) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 2
(-2.9)
A 		1.8
(-3)
A 		11.2
(3)
A 		1.8
(-3)
A 		9
B 1.8
(0.8)
A 		2.2
(3)
A 		1.2
(-2.5)
A 		1.1
(-3)
A 		1
C 2.9
(3)
A 		2.4
(1.6)
A 		1
(-2.2)
A 		0.7
(-3)
A 		2
avr 2.2
(-1.2) 		2.1
(-1.4) 		4.5
(3) 		1.2
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 9.8
(-0.4)
A 		3.6
(-2.2)
A 		0.9
(-3)
A 		21.3
(3)
A 		2.1
(-2.6)
A 		1.4
(-2.9)
A 		20
B 2.7
(-0.8)
A 		2.1
(-3)
A 		3.1
(0.8)
A 		3.7
(3)
A 		2.2
(-2.6)
A 		2.8
(-0.4)
A 		2
C 3.4
(3)
A 		1.2
(-3)
A 		1.4
(-2.5)
A 		3
(1.9)
A 		2.1
(-0.5)
A 		1.7
(-1.6)
A 		2
avr 5.3
(-0.2) 		2.3
(-2.6) 		1.8
(-3) 		9.3
(3) 		2.1
(-2.8) 		2
(-2.8) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193625_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17555
Sequence Type Exemplar sequence
Representative Public ID F20D1.4 NCBI
Target Description F20D1.4 /REP_DB=WormBase Gene ID /WP=CE09497 /TR=Q93537 /GB=CAB01747.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=transcriptional activator protein PUR-alpha like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078088(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078088 NCBI Caenorhabditis elegans F20D1.4 (F20D1.4) mRNA, complete cds. 11/11 A 
GENEFINDER00000040739 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14980870:14982720:-1 9/11 None
SNAP00000040733 ENSEMBL cdna:SNAP chromosome:CEL140:X:14982093:14982720:-1 9/11 None
F20D1.4 ENSEMBL cdna:known chromosome:CEL140:X:14982056:14982720:-1 gene:F20D1.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14982055-14982720(-) 84.8 100.0
Public Domain and Genome References
Gene Title transcriptional activator protein PUR-alpha like
Ensembl F20D1.4
Entrez Gene 184719 Entrez gene
SwissProt Q93537 EMBL-EBI
WormBase CE09497 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01747 Hypothetical protein F20D1.4 [Caenorhabditis elegans] ref|NP_510489.1| F20D1.4 [Caenorhabditis elegans] 1.0E-99
blast CAB01747 Hypothetical protein F20D1.4 [Caenorhabditis elegans] ref|NP_510489.1| F20D1.4 [Caenorhabditis elegans] 7.0E-69
blast CAB01747 Hypothetical protein F20D1.4 [Caenorhabditis elegans] ref|NP_510489.1| F20D1.4 [Caenorhabditis elegans] 3.0E-68
blast AAK29813 Adp-ribosylation factor related protein 1.1 [Caenorhabditis elegans] ref|NP_501242.1| ADP-Ribosylation Factor related family member (arf-1.1) [Caenorhabditis elegans] sp|Q94231|ARL6_CAEEL ADP-ribosylation factor-like protein 9.0E-61
blast AAK29814 Pur alpha like protein protein 1 [Caenorhabditis elegans] ref|NP_501241.1| Pur alpha Like Protein family member (plp-1) [Caenorhabditis elegans] 9.0E-58
blast AAK29814 Pur alpha like protein protein 1 [Caenorhabditis elegans] ref|NP_501241.1| Pur alpha Like Protein family member (plp-1) [Caenorhabditis elegans] 3.0E-57
blast CAE61929 Hypothetical protein CBG05926 [Caenorhabditis briggsae] 5.0E-55
blast AAK29814 Pur alpha like protein protein 1 [Caenorhabditis elegans] ref|NP_501241.1| Pur alpha Like Protein family member (plp-1) [Caenorhabditis elegans] 2.0E-42
Protein Domains
Database ID Description E-Value
Pfam IPR006628 EMBL-EBI PUR-alpha/beta/gamma, DNA/RNA-binding 1.1E-45
Pfam IPR006689 EMBL-EBI ARF/SAR superfamily 1.1E-54
Pfam IPR006628 EMBL-EBI PUR-alpha/beta/gamma, DNA/RNA-binding 1.1E-58
Sequence
Target Sequence 
>C. ELEGANS:193625_AT
tgtggagtacacgcgcttttccacgcggtgtacggcaagcgattatcaatggagcgcgaa
agccgcaaggggagtccagaagcccgtattgcagccaaaggaaagctctctcatcaaatc
agagaaaattctacgtcgatctgaaggaaaatgatcgtggtcgctttttgcgcattgctc
cgatgaacccacgtcaaactcgccaacaaatcgccattccatccgatggaatcgccgaaa
ttcataaggtgctaaccgaatatttggccaagttcggagagggacacgaacaggagaaca
ccaacactccaaaaatcactgctgaaaacagaacgttccttttccattccggaaagaacg
atcgtggagagtttgttcgcatctcggaggaaataattttgtgtcaaatagaagtttatt
ctaattttatattcagatcaaactcaacagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGGAGTACACGCGCTTTTCCACG 305 551 14 Antisense
CTTTTCCACGCGGTGTACGGCAAGC 562 219 29 Antisense
GCGGTGTACGGCAAGCGATTATCAA 501 291 38 Antisense
AATGGAGCGCGAAAGCCGCAAGGGG 572 157 61 Antisense
CGCGAAAGCCGCAAGGGGAGTCCAG 166 255 68 Antisense
GCCGCAAGGGGAGTCCAGAAGCCCG 497 285 75 Antisense
TCCAGAAGCCCGTATTGCAGCCAAA 656 587 88 Antisense
GCCCGTATTGCAGCCAAAGGAAAGC 423 277 95 Antisense
TCACTGCTGAAAACAGAACGTTCCT 266 625 329 Antisense
TGTTCGCATCTCGGAGGAAATAATT 45 563 387 Antisense
TTATATTCAGATCAAACTCAACAGC 1 679 440 Antisense
Sequence Source Affymetrix Proprietary Database