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[193621_s_at]

Description F55C7.7A /REP_DB=WormBase Gene ID /WP=CE19464 /GEN=unc-73 /TR=O61528 /GB=AAC71108.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=guanine nucleotide exchange factor
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 05      4grp_high_8_8 > 32      6grp_highest_nonorm > 90      6grp_total > 04      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 433
1 2 3 4 range
A 503.7
(3)
P 		93.4
(-3)
P 		97.9
(-2.9)
P 		123
(-2.6)
P 		410
B 362.2
(3)
P 		135.5
(-1.7)
P 		70.3
(-3)
A 		130.8
(-1.8)
P 		292
C 313.8
(3)
P 		85.5
(-3)
P 		91.2
(-2.9)
P 		141.7
(-1.5)
P 		228
avr 393.2
(3) 		104.8
(-2.6) 		86.5
(-3) 		131.8
(-2.1) 		307

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 244
1 2 3 4 5 6 range
A 211.5
(3)
P 		207.2
(2.8)
P 		143.4
(-0.2)
P 		172.9
(1.2)
P 		129
(-0.9)
P 		83.6
(-3)
P 		128
B 262.4
(3)
P 		189.4
(1)
P 		101.5
(-1.5)
P 		103.2
(-1.4)
P 		133.3
(-0.6)
P 		46.9
(-3)
P 		216
C 290.7
(3)
P 		236.6
(1.4)
P 		184.2
(-0.1)
P 		127.5
(-1.8)
P 		123.3
(-1.9)
P 		87.7
(-3)
P 		203
avr 254.9
(3) 		211.1
(1.6) 		143
(-0.7) 		134.5
(-1) 		128.5
(-1.2) 		72.7
(-3) 		182
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 183.4
(3)
P 		20.3
(-2.9)
P 		25.6
(-2.7)
P 		18
(-3)
P 		165
B 115.5
(3)
P 		33.5
(-2)
P 		16.3
(-3)
A 		27.9
(-2.3)
P 		99
C 103.9
(3)
P 		24.6
(-2.9)
P 		31.8
(-2.4)
P 		23.9
(-3)
P 		80
avr 134.3
(3) 		26.1
(-2.8) 		24.6
(-2.9) 		23.3
(-3) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 39
(-0.9)
P 		73.7
(3)
P 		31.2
(-1.8)
P 		43.9
(-0.4)
P 		38.9
(-0.9)
P 		20.4
(-3)
P 		53
B 91.3
(3)
P 		63
(0.7)
P 		24.7
(-2.4)
P 		27.3
(-2.2)
P 		54.1
(-0)
P 		17.5
(-3)
P 		74
C 107.8
(3)
P 		72.2
(0.4)
P 		46.9
(-1.4)
P 		25.6
(-3)
P 		39.9
(-2)
P 		29.3
(-2.7)
P 		82
avr 79.4
(3) 		69.6
(2) 		34.3
(-1.7) 		32.3
(-2) 		44.3
(-0.7) 		22.4
(-3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193621_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.110
Sequence Type Exemplar sequence
Representative Public ID F55C7.7A NCBI
Target Description F55C7.7A /REP_DB=WormBase Gene ID /WP=CE19464 /GEN=unc-73 /TR=O61528 /GB=AAC71108.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=guanine nucleotide exchange factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026325(11), NM_001026327(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026325 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 11/11 None
NM_001026327 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 11/11 None
GENEFINDER00000014634 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3999058:4001912:-1 11/11 None
SNAP00000014626 ENSEMBL cdna:SNAP chromosome:CEL140:I:3999350:4000895:-1 11/11 None
F55C7.7a ENSEMBL cdna:known chromosome:CEL140:I:3999350:4031628:-1 gene:F55C7.7 11/11 A 
F55C7.7c ENSEMBL cdna:known chromosome:CEL140:I:3999350:4020543:-1 gene:F55C7.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171988 6 2 NM_001026325 172976_s_at (11), 186050_s_at (11), 190347_s_at (11), 193621_s_at (11)
NM_001026326 190347_s_at (11)
NM_001026327 172976_s_at (11), 186050_s_at (11), 193621_s_at (11)
NM_001026328 186050_s_at (11)
NM_001026329 186050_s_at (11)
NM_001026330 186050_s_at (9), 190347_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3999349-4029229(-) 99.72 99.72
Public Domain and Genome References
Ensembl F55C7.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71108 Uncoordinated protein 73, isoform a [Caenorhabditis elegans] ref|NP_001021496.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] gb|AAC12931.1| guanine nucleotide exchange factor UNC-73A [Caenorhabditis elegans] 0.0
blast AAT68892 Uncoordinated protein 73, isoform f [Caenorhabditis elegans] ref|NP_001021501.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
blast AAC71109 Uncoordinated protein 73, isoform b [Caenorhabditis elegans] ref|NP_001021497.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] gb|AAC12932.1| guanine nucleotide exchange factor UNC-73B [Caenorhabditis elegans] 0.0
blast AAC71110 Uncoordinated protein 73, isoform c [Caenorhabditis elegans] ref|NP_001021498.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
blast CAE66729 Hypothetical protein CBG12078 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.7E-9
Pfam IPR000219 EMBL-EBI DH domain 2.4E-18
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000014634 2 24-43,50-72
Sequence
Target Sequence 
>C. ELEGANS:193621_S_AT
gtgcatttgatactgccacgtcatcaacggacaattttgacgttcttctggagttgacga
catgtgagagaccagttgtggttgaggatatgaaagatattgaagttgtcgagggaaatg
atgttgaaatgtgtccgattatttcttcgcatacagacttcaccgtaatatggcatggac
cagcggttgactcaaaacgagctagaatccagacaaatcaactgaacagtcgacttttga
ttaaacacgtaaaaaagtgtgatgccggtgcttattcagtaatagcaaaaaactcatttg
gagtgacgtccactgtcgcatttctcagtgttatttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCATTTGATACTGCCACGTCATC 509 479 6854 Antisense
GTTCTTCTGGAGTTGACGACATGTG 694 443 6895 Antisense
AAATGTGTCCGATTATTTCTTCGCA 121 121 6980 Antisense
ATTTCTTCGCATACAGACTTCACCG 97 15 6994 Antisense
GACTTCACCGTAATATGGCATGGAC 467 371 7009 Antisense
GGCATGGACCAGCGGTTGACTCAAA 67 535 7025 Antisense
AAACGAGCTAGAATCCAGACAAATC 55 127 7048 Antisense
GAACAGTCGACTTTTGATTAAACAC 439 347 7077 Antisense
GTGTGATGCCGGTGCTTATTCAGTA 611 487 7110 Antisense
TTTGGAGTGACGTCCACTGTCGCAT 467 665 7150 Antisense
GTCGCATTTCTCAGTGTTATTTCAA 294 475 7168 Antisense
Sequence Source Affymetrix Proprietary Database