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[193611_at] glutathione S-transferase

Description R107.7 /REP_DB=WormBase Gene ID /WP=CE00302 /GEN=gst-1 /TR=SW:P10299 /GB=CAA78471.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Glutathione S-transferase P subunit
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 66      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 37      6grp_total > 66      4grp_total > 01      6grp_highest_sub > In      6grp_cgc > 47      6grp_high1_sub > Da      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10987
1 2 3 4 range
A 4749.4
(-2.9)
P 		4500.1
(-3)
P 		5392.2
(-2.5)
P 		14728
(3)
P 		10228
B 4725.9
(-2.4)
P 		3741.2
(-3)
P 		5592.3
(-1.9)
P 		13740.6
(3)
P 		9999
C 4780.2
(-2.6)
P 		4163.4
(-3)
P 		5123.8
(-2.4)
P 		14420.5
(3)
P 		10257
avr 4751.8
(-2.6) 		4134.9
(-3) 		5369.4
(-2.3) 		14296.4
(3) 		10162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3785
1 2 3 4 5 6 range
A 4954.7
(-0.9)
P 		3802.2
(-3)
P 		5886.1
(0.9)
P 		5099
(-0.6)
P 		4309
(-2.1)
P 		7042.6
(3)
P 		3240
B 5037.2
(-0.6)
P 		4004.5
(-3)
P 		6055
(1.8)
P 		4573.8
(-1.7)
P 		4565.4
(-1.7)
P 		6575.9
(3)
P 		2571
C 4705.7
(-1.7)
P 		3875.8
(-3)
P 		4966.4
(-1.2)
P 		4192.5
(-2.5)
P 		4809.6
(-1.5)
P 		7586.8
(3)
P 		3711
avr 4899.2
(-1.1) 		3894.2
(-3) 		5635.8
(0.3) 		4621.8
(-1.6) 		4561.3
(-1.7) 		7068.4
(3) 		3174
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2004
1 2 3 4 range
A 1729.3
(0.8)
P 		976.8
(-3)
P 		1409.6
(-0.8)
P 		2155.9
(3)
P 		1179
B 1507.1
(-1.3)
P 		924
(-3)
P 		1295.9
(-1.9)
P 		2928.1
(3)
P 		2004
C 1582.4
(-1.1)
P 		1197
(-3)
P 		1785.8
(-0.1)
P 		2431.5
(3)
P 		1235
avr 1606.3
(-0.7) 		1032.6
(-3) 		1497.1
(-1.1) 		2505.2
(3) 		1473

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1697
1 2 3 4 5 6 range
A 913.4
(-3)
P 		1352.2
(0.3)
P 		1281.9
(-0.2)
P 		1293.2
(-0.2)
P 		1300.3
(-0.1)
P 		1714.7
(3)
P 		801
B 1752.7
(-0.4)
P 		1332.9
(-2.4)
P 		1470.3
(-1.7)
P 		1211.5
(-3)
P 		1854.8
(0.1)
P 		2452.1
(3)
P 		1241
C 1744.2
(0.2)
P 		1182.6
(-1.8)
P 		1264
(-1.5)
P 		843.1
(-3)
P 		1556.3
(-0.5)
P 		2539.7
(3)
P 		1697
avr 1470.1
(-1.1) 		1289.2
(-2.1) 		1338.7
(-1.8) 		1115.9
(-3) 		1570.5
(-0.6) 		2235.5
(3) 		1120
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193611_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7774
Sequence Type Exemplar sequence
Representative Public ID R107.7 NCBI
Target Description R107.7 /REP_DB=WormBase Gene ID /WP=CE00302 /GEN=gst-1 /TR=SW:P10299 /GB=CAA78471.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Glutathione S-transferase P subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066605(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066605 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-1) (gst-1) mRNA, complete cds. 11/11 None
SNAP00000003028 ENSEMBL cdna:SNAP chromosome:CEL140:III:9059263:9059991:1 11/11 None
GENEFINDER00000003035 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9059263:9059991:1 11/11 None
R107.7 ENSEMBL cdna:known chromosome:CEL140:III:9059231:9060090:1 gene:R107.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9059258-9059987(+) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-1
Ensembl R107.7
Entrez Gene 176281 Entrez gene
SwissProt P10299 EMBL-EBI
WormBase CE00302 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1592145_S_AT similar to glutathione S-transferase, pi 1 cfa
CANINE:1592122_S_AT similar to glutathione S-transferase, pi 1 cfa
CANINE:1591553_AT similar to glutathione S-transferase, pi 1 cfa
CANINE:1586712_AT similar to glutathione S-transferase, pi 1 cfa
CANINE:1583739_S_AT similar to glutathione S-transferase, pi 1 cfa
CANINE_2:CFA.6206.1.A1_A_AT similar to glutathione S-transferase, pi 1 cfa
CANINE_2:CFAAFFX.17685.1.S1_S_AT similar to glutathione S-transferase, pi 1 cfa
MU11KSUBA:D30687_F_AT glutathione S-transferase, pi 1 mm
MG-U74AV2:99583_AT glutathione S-transferase, pi 1 mm
MOE430A:1449575_A_AT glutathione S-transferase, pi 1 mm
MOUSE430A_2:1449575_A_AT glutathione S-transferase, pi 1 mm
MOUSE430_2:1449575_A_AT glutathione S-transferase, pi 1 mm
MU19KSUBB:TC26663_AT Glutathione S-transferase, pi 1, mRNA (cDNA clone MGC:6054 IMAGE:3593257) mm
RT-U34:RC_AI012589_S_AT glutathione-S-transferase, pi 1 rn
RG-U34A:RC_AI012589_S_AT glutathione-S-transferase, pi 1 rn
RT-U34:X02904CDS_S_AT glutathione-S-transferase, pi 1 rn
RG-U34A:X02904CDS_S_AT glutathione-S-transferase, pi 1 rn
RAE230A:1388122_AT glutathione-S-transferase, pi 1 rn
RAT230_2:1388122_AT glutathione-S-transferase, pi 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4364 glutathione transferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA78471 Hypothetical protein R107.7 [Caenorhabditis elegans] emb|CAA31979.1| unnamed protein product [Caenorhabditis elegans] ref|NP_499006.1| Glutathione S-Transferase family member (gst-1) [Caenorhabditis elegans] sp|P10299|GSTP1_CAEEL Glutathione S-transferase P (GST class-pi) 1.0E-119
blast CAE62680 Hypothetical protein CBG06825 [Caenorhabditis briggsae] 1.0E-101
blast AAC16998 Glutathione s-transferase protein 25 [Caenorhabditis elegans] ref|NP_490857.1| Glutathione S-Transferase family member (gst-25) [Caenorhabditis elegans] 1.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 6.2E-23
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 4.8E-24
Sequence
Target Sequence 
>C. ELEGANS:193611_AT
caaagatgatcttcggccaggttccatgtcttctatccggagacgaggagattgttcaat
ctggagctatcatccgtcatctcgctcgtcttaatgggctcaatggctccaacgagacag
agacaactttcatcgacatgttctacgaaggacttcgtgatttgcacaccaagtacacca
ctatgatctacagaaactacgaagacggcaaggctccgtacatcaaggacgttcttccag
gagagctcgctcgtctggagaagcttttccatacctacaagaacggagagcactacgtta
ttggagacaaggaaagctatgcggattatgtgctgttcgaggagctcgacattcatttga
ttctcacaccaaatgctcttgatggtgttccagcactcaagaagttccacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAGATGATCTTCGGCCAGGTTCC 286 193 140 Antisense
GTTCCATGTCTTCTATCCGGAGACG 458 441 160 Antisense
AATCTGGAGCTATCATCCGTCATCT 608 169 197 Antisense
TCTCGCTCGTCTTAATGGGCTCAAT 617 609 219 Antisense
TGGGCTCAATGGCTCCAACGAGACA 552 545 234 Antisense
GAAGGACTTCGTGATTTGCACACCA 120 335 286 Antisense
AAGGCTCCGTACATCAAGGACGTTC 505 145 349 Antisense
AGAGCTCGCTCGTCTGGAGAAGCTT 136 71 381 Antisense
GAGGAGCTCGACATTCATTTGATTC 638 403 478 Antisense
GATTCTCACACCAAATGCTCTTGAT 4 433 498 Antisense
GTTCCAGCACTCAAGAAGTTCCACG 398 441 526 Antisense
Sequence Source Affymetrix Proprietary Database