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[193607_at] HECT-domain (ubiquitin-transferase).

Description Y39A1C.2 /REP_DB=WormBase Gene ID /WP=CE19147 /GEN=oxi-1 /TR=Q9XXE6 /GB=CAA19508.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=HECT-domain (ubiquitin-transferase).
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_high_8_8 > 40      6grp_highest_nonorm > 82      6grp_total > 59      4grp_total > 53      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 295
1 2 3 4 range
A 395.1
(3)
P 		339
(1.4)
P 		293.1
(0)
P 		190.5
(-3)
A 		205
B 452.4
(3)
P 		408.6
(1)
M 		409.5
(1.1)
P 		318.8
(-3)
P 		134
C 485.9
(3)
P 		413
(0.5)
A 		339.7
(-1.9)
P 		308.4
(-3)
P 		178
avr 444.5
(3) 		386.9
(1) 		347.4
(-0.4) 		272.6
(-3) 		172

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 349.1
(-0.5)
P 		442
(2.4)
P 		266.8
(-3)
P 		359.6
(-0.1)
P 		326.7
(-1.2)
P 		461.7
(3)
P 		195
B 413
(2.4)
P 		324.6
(-0.7)
P 		287.3
(-2.1)
P 		260.6
(-3)
P 		323.2
(-0.8)
P 		429.6
(3)
P 		169
C 362
(0.3)
P 		304.2
(-2.6)
P 		375.9
(0.9)
P 		296
(-3)
P 		417.4
(3)
P 		362.9
(0.3)
P 		121
avr 374.7
(0.7) 		356.9
(-0.3) 		310
(-2.8) 		305.4
(-3) 		355.8
(-0.3) 		418.1
(3) 		113
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 143.8
(3)
P 		73.6
(-0.6)
P 		76.6
(-0.5)
P 		27.9
(-3)
A 		116
B 144.3
(3)
P 		100.9
(-0.4)
M 		94.9
(-0.9)
P 		67.9
(-3)
P 		76
C 160.8
(3)
P 		118.7
(0.7)
A 		118.4
(0.7)
P 		52
(-3)
P 		109
avr 149.6
(3) 		97.7
(-0.1) 		96.6
(-0.2) 		49.3
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 64.3
(-2.6)
P 		157.2
(3)
P 		58.1
(-3)
P 		91.2
(-1)
P 		98.6
(-0.5)
P 		112.4
(0.3)
P 		99
B 143.7
(1.9)
P 		108
(-0.4)
P 		69.8
(-2.9)
P 		69
(-3)
P 		131.3
(1.1)
P 		160.2
(3)
P 		91
C 134.2
(2.9)
P 		92.8
(-0.4)
P 		95.7
(-0.1)
P 		59.5
(-3)
P 		135.1
(3)
P 		121.5
(1.9)
P 		76
avr 114.1
(1.2) 		119.3
(1.8) 		74.5
(-2.9) 		73.2
(-3) 		121.7
(2) 		131.4
(3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193607_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8000
Sequence Type Exemplar sequence
Representative Public ID Y39A1C.2 NCBI
Target Description Y39A1C.2 /REP_DB=WormBase Gene ID /WP=CE19147 /GEN=oxi-1 /TR=Q9XXE6 /GB=CAA19508.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=HECT-domain (ubiquitin-transferase).
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066991(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066991 NCBI Caenorhabditis elegans OXidative stress Induced family member (oxi-1) (oxi-1) mRNA, complete cds. 11/11 None
SNAP00000042755 ENSEMBL cdna:SNAP chromosome:CEL140:III:10858190:10866743:1 11/11 None
GENEFINDER00000042760 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10858190:10866743:1 11/11 None
Y39A1C.2 ENSEMBL cdna:known chromosome:CEL140:III:10858161:10866820:1 gene:Y39A1C.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10858185-10866739(+) 100.0 100.0
Public Domain and Genome References
Gene Title HECT-domain (ubiquitin-transferase).
Gene Symbol oxi-1
Ensembl Y39A1C.2
Entrez Gene 176515 Entrez gene
SwissProt Q9XXE6 EMBL-EBI
WormBase CE19147 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251998_AT HECT-domain-containing protein / ubiquitin-transferase family protein / IQ calmodulin-binding motif-containing protein at
CANINE_2:CFAAFFX.16987.1.S1_AT similar to ubiquitin protein ligase E3B isoform a cfa
DROSOPHILA_2:1623955_AT dm
DROSGENOME1:148439_AT dm
CHICKEN:GGAAFFX.21410.1.S1_S_AT ubiquitin protein ligase E3B gga
HG-U133A:212403_AT ubiquitin protein ligase E3B hs
HG-U133A_2:212403_AT ubiquitin protein ligase E3B hs
HG-U133_PLUS_2:212403_AT ubiquitin protein ligase E3B hs
HG-U133_PLUS_2:213822_S_AT ubiquitin protein ligase E3B hs
HG-U133A_2:213822_S_AT ubiquitin protein ligase E3B hs
HG-U133A:213822_S_AT ubiquitin protein ligase E3B hs
HG-U133_PLUS_2:232301_AT ubiquitin protein ligase E3B hs
HG-U133B:232301_AT ubiquitin protein ligase E3B hs
HU35KSUBA:RC_AA609285_AT ubiquitin protein ligase E3B hs
HU35KSUBA:RC_AA398368_AT ubiquitin protein ligase E3B hs
HU35KSUBA:AA312078_AT ubiquitin protein ligase E3B hs
HU35KSUBD:RC_W90608_AT ubiquitin protein ligase E3B hs
HU35KSUBD:RC_AA486538_AT ubiquitin protein ligase E3B hs
U133_X3P:HS.305834.0.S1_3P_X_AT ubiquitin protein ligase E3B hs
U133_X3P:HS.305834.0.S1_3P_AT ubiquitin protein ligase E3B hs
U133_X3P:HS.17639.3.A1_3P_AT ubiquitin protein ligase E3B hs
U133_X3P:HS.17639.1.A2_3P_AT ubiquitin protein ligase E3B hs
U133_X3P:HS.17639.1.A1_3P_A_AT ubiquitin protein ligase E3B hs
U133_X3P:HS.17639.0.S1_3P_S_AT ubiquitin protein ligase E3B hs
U133_X3P:HS.17639.0.S1_3P_A_AT ubiquitin protein ligase E3B hs
U133_X3P:232301_3P_AT ubiquitin protein ligase E3B hs
HG-U133A_2:212404_S_AT ubiquitin protein ligase E3B hs
HG-U133_PLUS_2:212404_S_AT ubiquitin protein ligase E3B hs
HG-U133A:212404_S_AT ubiquitin protein ligase E3B hs
HG-U133_PLUS_2:228860_AT ubiquitin protein ligase E3B hs
HG-U133B:228860_AT ubiquitin protein ligase E3B hs
HG-U95AV2:40622_R_AT ubiquitin protein ligase E3B hs
HG-U95AV2:40623_AT ubiquitin protein ligase E3B hs
HG-U95C:61889_AT ubiquitin protein ligase E3B hs
HG-U95C:63981_AT ubiquitin protein ligase E3B hs
HG-U95E:67489_AT ubiquitin protein ligase E3B hs
HG-U133_PLUS_2:227914_S_AT Ubiquitin protein ligase E3B hs
HG-U133B:227914_S_AT Ubiquitin protein ligase E3B hs
HU35KSUBB:RC_W78138_AT Ubiquitin protein ligase E3B hs
U133_X3P:HS.17639.2.A1_3P_AT Ubiquitin protein ligase E3B hs
MG-U74BV2:109077_AT ubiquitin protein ligase E3B mm
MOE430A:1452149_AT ubiquitin protein ligase E3B mm
MOUSE430A_2:1452149_AT ubiquitin protein ligase E3B mm
MOUSE430_2:1452149_AT ubiquitin protein ligase E3B mm
MG-U74BV2:109078_AT ubiquitin protein ligase E3B mm
MU19KSUBA:TC19899_AT Ubiquitin protein ligase E3B (Ube3b), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA19508 Hypothetical protein Y39A1C.2 [Caenorhabditis elegans] ref|NP_499392.1| OXidative stress Induced family member (oxi-1) [Caenorhabditis elegans] 0.0
blast CAE71392 Hypothetical protein CBG18299 [Caenorhabditis briggsae] 0.0
blast BAA21847.1 E6-AP ubiquitin-protein ligase [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 5.2E-48
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 2.2E-15
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 5.2E-48
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 2.2E-15
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 5.2E-48
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 2.2E-15
Sequence
Target Sequence 
>C. ELEGANS:193607_AT
tatgtgcaccgaatggctcatcaccgagtattccgacgaacccaggaacagtgcaaggcg
ttcgtcaccggaatgcaatcgatccttcagccgacgtggctgtcactgttcgctccgaat
gatttgcaatgtctgattagtggagtcaattcagacatcgatctggcggatctgaagcgc
aatgtgcaatatttcggaggattccatggaaaccatcggcttattaaatggctttgggat
attcttgagaacaagttcacttcagaagagaggaaactgttcctaaagtttgtcacttct
tgctcgagacctcctgttcttggattttcgtatctcgagccgcccttctccattcgatgc
gttgaagtttccgacgatcaggatcagggagacaccttgggaagtgtggttcgtggattc
ttggctttgagaaagggaactgcagctactcgacttccaactgcgtccacctgctttaac
ttgctaaagcttccgaattacaataagaagtcactgctgctcgagaaacttcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGTGCACCGAATGGCTCATCACC 212 655 2641 Antisense
TCATCACCGAGTATTCCGACGAACC 431 619 2658 Antisense
GAACAGTGCAAGGCGTTCGTCACCG 336 351 2686 Antisense
ACCGGAATGCAATCGATCCTTCAGC 318 89 2707 Antisense
ATCCTTCAGCCGACGTGGCTGTCAC 572 39 2722 Antisense
GTCACTGTTCGCTCCGAATGATTTG 318 465 2742 Antisense
AAGTTTGTCACTTCTTGCTCGAGAC 586 155 2926 Antisense
AGACCTCCTGTTCTTGGATTTTCGT 383 69 2947 Antisense
GATGCGTTGAAGTTTCCGACGATCA 180 415 2996 Antisense
AAAGGGAACTGCAGCTACTCGACTT 103 121 3072 Antisense
GTCACTGCTGCTCGAGAAACTTCGT 398 465 3150 Antisense
Sequence Source Affymetrix Proprietary Database