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[193603_s_at]

Description W01D2.2B /REP_DB=WormBase Gene ID /WP=CE20125 /GEN=nhr-61 /TR=Q9U355 /GB=CAB05637.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Zinc finger, C4 type (two domains)
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 05      4grp_highest_8_8 > 30      4grp_high_8_8 > 04      6grp_highest_nonorm > 71      6grp_total > 04      4grp_total > 04      6grp_highest_sub > De      6grp_cgc > 51      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 360
1 2 3 4 range
A 272.4
(-2)
P 		226.6
(-2.8)
P 		218.3
(-3)
P 		542.1
(3)
P 		324
B 321.5
(-0.5)
P 		182.4
(-3)
P 		254
(-1.7)
P 		520.1
(3)
P 		338
C 284.3
(-1.6)
P 		234.5
(-2.6)
P 		212.1
(-3)
P 		524.4
(3)
P 		312
avr 292.7
(-1.5) 		214.5
(-3) 		228.1
(-2.7) 		528.9
(3) 		314

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 197
1 2 3 4 5 6 range
A 383.5
(3)
P 		316.9
(0.3)
P 		374.9
(2.7)
P 		326.8
(0.7)
P 		317.2
(0.4)
P 		233
(-3)
P 		151
B 430.2
(3)
P 		352.5
(-0.8)
P 		328.7
(-1.9)
P 		379.6
(0.5)
P 		419.8
(2.5)
P 		306.8
(-3)
P 		123
C 297.7
(0.1)
P 		353.5
(3)
P 		277
(-0.9)
P 		242.8
(-2.7)
P 		295.6
(0)
P 		237.2
(-3)
P 		116
avr 370.5
(3) 		341
(1.4) 		326.9
(0.7) 		316.4
(0.1) 		344.2
(1.6) 		259
(-3) 		112
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 99.2
(3)
P 		49.2
(-3)
P 		57.1
(-2.1)
P 		79.4
(0.6)
P 		50
B 102.5
(2.2)
P 		45.1
(-3)
P 		58.9
(-1.7)
P 		110.8
(3)
P 		66
C 94.1
(3)
P 		67.4
(-3)
P 		73.9
(-1.5)
P 		88.4
(1.7)
P 		27
avr 98.6
(3) 		53.9
(-3) 		63.3
(-1.7) 		92.9
(2.2) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 70.7
(-1.5)
P 		112.7
(3)
P 		81.7
(-0.3)
P 		82.9
(-0.2)
P 		95.7
(1.2)
P 		56.7
(-3)
P 		56
B 149.7
(1.6)
P 		117.3
(-0.5)
P 		79.8
(-3)
P 		100.5
(-1.6)
P 		170.6
(3)
P 		114.4
(-0.7)
P 		91
C 110.3
(3)
P 		107.9
(2.8)
P 		70.5
(-0.9)
P 		48.8
(-3)
P 		95.7
(1.6)
P 		79.4
(-0)
P 		62
avr 110.2
(1.5) 		112.6
(1.9) 		77.3
(-3) 		77.4
(-3) 		120.7
(3) 		83.5
(-2.1) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193603_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3720
Sequence Type Exemplar sequence
Representative Public ID W01D2.2B NCBI
Target Description W01D2.2B /REP_DB=WormBase Gene ID /WP=CE20125 /GEN=nhr-61 /TR=Q9U355 /GB=CAB05637.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Zinc finger, C4 type (two domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027210(11), NM_001027211(11), AF273812(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027210 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-61) (nhr-61) mRNA, complete cds. 11/11 None
NM_001027211 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-61) (nhr-61) mRNA, complete cds. 11/11 None
AF273812 NCBI Caenorhabditis elegans clone yk70c6 nuclear receptor NHR-61 mRNA, partial cds. 11/11 None
SNAP00000015970 ENSEMBL cdna:SNAP chromosome:CEL140:II:14816135:14825383:-1 11/11 None
GENEFINDER00000015972 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14816135:14818431:-1 11/11 None
W01D2.2a ENSEMBL cdna:known chromosome:CEL140:II:14816026:14818432:-1 gene:W01D2.2 11/11 None
W01D2.2b ENSEMBL cdna:known chromosome:CEL140:II:14816027:14818431:-1 gene:W01D2.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
AF273810 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14816131-14818410(-) 100.0 100.0
Public Domain and Genome References
Ensembl W01D2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR11975 nuclear receptor NHR-61 [Caenorhabditis elegans] 0.0
blast CAB05636 Hypothetical protein W01D2.2a [Caenorhabditis elegans] ref|NP_001022381.1| Nuclear Hormone Receptor family member (nhr-61) [Caenorhabditis elegans] sp|O62389|NHR61_CAEEL Nuclear hormone receptor family member nhr-61 0.0
blast AAG15160 nuclear receptor NHR-61 [Caenorhabditis elegans] 0.0
blast AAG15158 nuclear receptor NHR-61 [Caenorhabditis elegans] 0.0
blast CAB05637 Hypothetical protein W01D2.2b [Caenorhabditis elegans] ref|NP_001022382.1| Nuclear Hormone Receptor family member (nhr-61) [Caenorhabditis elegans] pir||T26057 hypothetical protein W01D2.2b - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 1.2E-9
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 2.7E-10
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 1.2E-9
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 7.0E-34
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 1.0E-9
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 1.0E-9
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 4.4E-41
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 1.2E-9
Sequence
Target Sequence 
>C. ELEGANS:193603_S_AT
gactttcgacgagcttttgtactatttgtggatattctaaaatcgattgatcagttgaga
gatgtgcaggaagatgataaaatgaagattgcaaaatcgagattcgcggcgttctactgg
tggttgtgctccacgtggagtgcaaaagcgggctgcaacggtgtatgctattccaatgga
tcttatcatccggcttcgatcagtgatatgccaaagtccgagggaaagcacggagttcga
attgagtatgtcgactatagtggtgtgtcacagaagagcttggagaatttggtggagccg
ctgagacgtatggagctgtctgatgaggagaggatcgttggagctgtgatggttatttta
gctgatcctgtaccaaatgttagcaccaaaactgaaaagattcttgctgaagctcgggat
ttctacttggagcttctgggttattgcatcaagctgccagaggagcagcaaggcatcaga
gtatcaactatggttcttctattggcatctataatggaattggtgcacttaactaccgac
aacattcaactatccgacgtactccatgttatcgatctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTTCGACGAGCTTTTGTACTAT 518 371 631 Antisense
TCGAGATTCGCGGCGTTCTACTGGT 417 603 727 Antisense
TACTGGTGGTTGTGCTCCACGTGGA 405 647 745 Antisense
AAGCGGGCTGCAACGGTGTATGCTA 617 149 776 Antisense
ATGGATCTTATCATCCGGCTTCGAT 9 53 806 Antisense
GGAGCCGCTGAGACGTATGGAGCTG 606 521 924 Antisense
TCGGGATTTCTACTTGGAGCTTCTG 154 605 1044 Antisense
TGGAGCTTCTGGGTTATTGCATCAA 179 549 1058 Antisense
TGCACTTAACTACCGACAACATTCA 593 575 1154 Antisense
AACATTCAACTATCCGACGTACTCC 684 99 1171 Antisense
GACGTACTCCATGTTATCGATCTGG 135 373 1186 Antisense
Sequence Source Affymetrix Proprietary Database