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[193594_at] guanine nucleotide-binding protein

Description F18G5.3 /REP_DB=WormBase Gene ID /WP=CE20692 /GEN=gpa-12 /TR=Q19572 /GB=AAA81081.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 63      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 62      6grp_total > 62      4grp_total > 47      6grp_highest_sub > 03_04      6grp_cgc > 75      6grp_high1_sub > 03      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 406
1 2 3 4 range
A 648.1
(3)
P 		309.2
(-2.1)
P 		325.9
(-1.9)
P 		249.7
(-3)
P 		398
B 637.5
(3)
P 		342.8
(-2.4)
P 		343.1
(-2.4)
P 		312.3
(-3)
P 		325
C 655.2
(3)
P 		306
(-2.7)
P 		287.7
(-3)
P 		367.7
(-1.7)
P 		368
avr 646.9
(3) 		319.3
(-2.8) 		318.9
(-2.8) 		309.9
(-3) 		337

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 336
1 2 3 4 5 6 range
A 276.9
(-3)
P 		448.5
(0.1)
P 		612.5
(3)
P 		562.8
(2.1)
P 		461.9
(0.3)
P 		435.9
(-0.2)
P 		336
B 290.2
(-3)
P 		445.6
(0.7)
P 		540.3
(3)
P 		497
(2)
P 		372.5
(-1)
P 		420
(0.1)
P 		250
C 337.4
(-3)
P 		407.5
(-1.5)
P 		564.5
(2)
P 		609.7
(3)
P 		358.2
(-2.5)
P 		419
(-1.2)
P 		272
avr 301.5
(-3) 		433.9
(-0.1) 		572.4
(3) 		556.5
(2.6) 		397.5
(-0.9) 		425
(-0.3) 		271
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 236
(3)
P 		67.1
(-2.1)
P 		85.2
(-1.5)
P 		36.5
(-3)
P 		200
B 203.3
(3)
P 		84.7
(-2.2)
P 		79.5
(-2.4)
P 		66.6
(-3)
P 		137
C 216.9
(3)
P 		88
(-2)
P 		100.3
(-1.5)
P 		62
(-3)
P 		155
avr 218.7
(3) 		79.9
(-2.1) 		88.3
(-1.8) 		55
(-3) 		164

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 51.1
(-3)
P 		159.5
(3)
P 		133.4
(1.6)
P 		142.7
(2.1)
P 		139.4
(1.9)
P 		106.1
(0)
P 		108
B 101
(-3)
P 		148.3
(2.1)
P 		131.2
(0.3)
P 		131.6
(0.3)
P 		151.4
(2.4)
P 		156.6
(3)
P 		56
C 125
(-1)
P 		124.3
(-1.2)
P 		143.7
(3)
P 		122.6
(-1.6)
P 		115.9
(-3)
P 		140.3
(2.3)
P 		28
avr 92.4
(-3) 		144
(3) 		136.1
(2.1) 		132.3
(1.6) 		135.6
(2) 		134.3
(1.9) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193594_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16078
Sequence Type Exemplar sequence
Representative Public ID F18G5.3 NCBI
Target Description F18G5.3 /REP_DB=WormBase Gene ID /WP=CE20692 /GEN=gpa-12 /TR=Q19572 /GB=AAA81081.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077156(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077156 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-12) (gpa-12) mRNA, complete cds. 11/11 None
SNAP00000036135 ENSEMBL cdna:SNAP chromosome:CEL140:X:9246947:9248847:1 11/11 None
GENEFINDER00000036145 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9246947:9248847:1 11/11 None
F18G5.3 ENSEMBL cdna:known chromosome:CEL140:X:9246937:9248847:1 gene:F18G5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9246946-9248847(+) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-12
Ensembl F18G5.3
Entrez Gene 181157 Entrez gene
SwissProt Q19572 EMBL-EBI
WormBase CE20692 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.25033.1.S1_AT similar to Guanine nucleotide-binding protein, alpha-12 subunit (G alpha 12) cfa
CANINE_2:CFAAFFX.25033.1.S1_S_AT similar to Guanine nucleotide-binding protein, alpha-12 subunit (G alpha 12) cfa
HU35KSUBC:RC_AA485431_S_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HU35KSUBD:RC_T16092_AT guanine nucleotide binding protein (G protein) alpha 12 hs
U133_X3P:HS.182874.1.S3_3P_AT guanine nucleotide binding protein (G protein) alpha 12 hs
U133_X3P:HS.182874.1.S2_3P_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U133A_2:221737_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-FOCUS:221737_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U133_PLUS_2:221737_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U133A:221737_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U133_PLUS_2:224681_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U133B:224681_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U95AV2:34106_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U95B:57079_AT guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U95B:44331_AT Guanine nucleotide binding protein (G protein) alpha 12 hs
HG-U95E:72170_AT Guanine nucleotide binding protein (G protein) alpha 12 hs
MG-U74AV2:97227_AT guanine nucleotide binding protein, alpha 12 mm
MG-U74AV2:97226_AT guanine nucleotide binding protein, alpha 12 mm
MU11KSUBA:M63659_S_AT guanine nucleotide binding protein, alpha 12 mm
MU11KSUBB:MSA.42550.0_S_AT guanine nucleotide binding protein, alpha 12 mm
MOE430A:1421026_AT guanine nucleotide binding protein, alpha 12 mm
MOUSE430A_2:1421026_AT guanine nucleotide binding protein, alpha 12 mm
MOUSE430_2:1421026_AT guanine nucleotide binding protein, alpha 12 mm
MOE430A:1450097_S_AT guanine nucleotide binding protein, alpha 12 mm
MOUSE430A_2:1450097_S_AT guanine nucleotide binding protein, alpha 12 mm
MOUSE430_2:1450097_S_AT guanine nucleotide binding protein, alpha 12 mm
MOE430A:1450096_AT guanine nucleotide binding protein, alpha 12 mm
MOUSE430A_2:1450096_AT guanine nucleotide binding protein, alpha 12 mm
MOUSE430_2:1450096_AT guanine nucleotide binding protein, alpha 12 mm
MOUSE430A_2:1439206_AT Guanine nucleotide binding protein, alpha 12 (Gna12), mRNA mm
MOE430A:1439206_AT Guanine nucleotide binding protein, alpha 12 (Gna12), mRNA mm
MOUSE430_2:1439206_AT Guanine nucleotide binding protein, alpha 12 (Gna12), mRNA mm
MOE430A:1455008_AT Guanine nucleotide binding protein, alpha 12 (Gna12), mRNA mm
MOUSE430A_2:1455008_AT Guanine nucleotide binding protein, alpha 12 (Gna12), mRNA mm
MOUSE430_2:1455008_AT Guanine nucleotide binding protein, alpha 12 (Gna12), mRNA mm
MU19KSUBA:TC22798_AT Guanine nucleotide binding protein, alpha 12 (Gna12), mRNA mm
RG-U34A:D85760_AT guanine nucleotide binding protein, alpha 12 rn
RG-U34A:S50461_S_AT guanine nucleotide binding protein, alpha 12 rn
RAE230A:1369278_AT guanine nucleotide binding protein, alpha 12 rn
RAT230_2:1369278_AT guanine nucleotide binding protein, alpha 12 rn
RAE230A:1371798_AT Guanine nucleotide binding protein, alpha 12 rn
RAT230_2:1371798_AT Guanine nucleotide binding protein, alpha 12 rn
RAE230B:1379926_AT Guanine nucleotide binding protein, alpha 12 rn
RAT230_2:1379926_AT Guanine nucleotide binding protein, alpha 12 rn
RAE230A:1389895_AT Guanine nucleotide binding protein, alpha 12 rn
RAT230_2:1389895_AT Guanine nucleotide binding protein, alpha 12 rn
RG-U34B:RC_AI013011_AT Guanine nucleotide binding protein, alpha 12 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81081 G protein, alpha subunit protein 12 [Caenorhabditis elegans] ref|NP_509557.1| G Protein, Alpha subunit family member (gpa-12) [Caenorhabditis elegans] gb|AAG32087.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q19572|GPA12_CAEEL Guanine nucleotide-binding protein alpha-12 subunit 0.0
blast AAW02896 gpa-12 [Caenorhabditis briggsae] sp|Q613V4|GPA12_CAEBR Guanine nucleotide-binding protein alpha-12 subunit emb|CAE69854.1| Hypothetical protein CBG16182 [Caenorhabditis briggsae] 0.0
blast D89605 protein F18G5.3 [imported] - Caenorhabditis elegans 1.0E-178
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193594_AT
gtgactcctgtcagtacttctttgagcatattccacgaatcgcaatgcctgatttttacc
caacaaaccgtgatatccttttctgccgaaaagcgacacgtggcatttctgaacacattt
tcgagatcaacaagattccatttaggttcattgatgttggcggacaaagatcgcaacgtc
aaaaatggtttcaatgttttacagacatcacaagtattttgttcatggtcgccagtaacg
aatacgatcaggtgattctcgaagacagacggacgaatcgagttgtcgaatcacggtctg
tttttgagacgattgtcaacaacagagcgttctctaatgtctccatcatcttgttcatga
acaaaaatgatttattgcaagagaaagttcccaaatccgatatcagacagtatttcacgg
atttcacaggggatcacactttagtccgagatgttcaatttttccttgtcgacaaattcg
aagcatctcgccgcgatcgtgcacgacccttcttttatcacttcaccactgccgtcgaca
ctgaaaacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGACTCCTGTCAGTACTTCTTTGA 387 483 461 Antisense
CAATGCCTGATTTTTACCCAACAAA 595 179 503 Antisense
AACAAACCGTGATATCCTTTTCTGC 614 133 522 Antisense
AGTATTTTGTTCATGGTCGCCAGTA 468 61 673 Antisense
GTTGTCGAATCACGGTCTGTTTTTG 17 437 742 Antisense
ACAGAGCGTTCTCTAATGTCTCCAT 147 109 782 Antisense
ATGTCTCCATCATCTTGTTCATGAA 306 49 797 Antisense
AGGGGATCACACTTTAGTCCGAGAT 342 57 888 Antisense
GATGTTCAATTTTTCCTTGTCGACA 693 409 910 Antisense
CGACAAATTCGAAGCATCTCGCCGC 275 253 930 Antisense
CCACTGCCGTCGACACTGAAAACAT 157 275 986 Antisense
Sequence Source Affymetrix Proprietary Database