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[193593_s_at]

Description M01D7.7 /REP_DB=WormBase Gene ID /WP=CE12272 /GEN=egl-30 /TR=Q17386 /GB=AAB58071.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 01      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 11      6grp_total > 14      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1079
1 2 3 4 range
A 1651.7
(3)
P 		1323.4
(0.8)
P 		1220.1
(0.1)
P 		763.2
(-3)
P 		889
B 1758.9
(3)
P 		1507.6
(1.5)
P 		1067.3
(-1.2)
P 		769.9
(-3)
P 		989
C 1449.5
(3)
P 		1066.7
(0)
P 		954
(-0.9)
P 		679.8
(-3)
P 		770
avr 1620
(3) 		1299.2
(0.8) 		1080.5
(-0.7) 		737.6
(-3) 		882

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1869
1 2 3 4 5 6 range
A 2324.2
(3)
P 		1154.4
(-0.8)
P 		1543.2
(0.5)
P 		743.5
(-2.1)
P 		1370.6
(-0.1)
P 		455.3
(-3)
P 		1869
B 2130.9
(3)
P 		1791.8
(1.4)
P 		1680.7
(0.9)
P 		1544.8
(0.3)
P 		1018.2
(-2.2)
P 		843.3
(-3)
P 		1288
C 1716.2
(2.8)
P 		1747
(3)
P 		1161.8
(-0.3)
P 		1451.9
(1.3)
P 		769
(-2.6)
P 		690.6
(-3)
P 		1056
avr 2057.1
(3) 		1564.4
(0.9) 		1461.9
(0.4) 		1246.7
(-0.5) 		1052.6
(-1.3) 		663.1
(-3) 		1394
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 490
1 2 3 4 range
A 601.4
(3)
P 		287.3
(-0.8)
P 		319
(-0.5)
P 		111.7
(-3)
P 		490
B 560.9
(3)
P 		372.3
(0.1)
P 		247.3
(-1.7)
P 		164.1
(-3)
P 		397
C 479.8
(3)
P 		306.7
(0.2)
P 		332.5
(0.6)
P 		114.6
(-3)
P 		365
avr 547.4
(3) 		322.1
(-0.2) 		299.6
(-0.6) 		130.1
(-3) 		417

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 631
1 2 3 4 5 6 range
A 428.4
(3)
P 		410.6
(2.7)
P 		336.1
(1.3)
P 		188.6
(-1.5)
P 		413.6
(2.7)
P 		110.9
(-3)
P 		318
B 741.5
(3)
P 		596.4
(1)
P 		408.1
(-1.7)
P 		409.2
(-1.7)
P 		413.7
(-1.6)
P 		314.5
(-3)
P 		427
C 636.1
(3)
P 		533.1
(1.5)
P 		295.7
(-2)
P 		292
(-2.1)
P 		248.8
(-2.7)
P 		231.2
(-3)
P 		405
avr 602
(3) 		513.4
(1.6) 		346.6
(-1) 		296.6
(-1.8) 		358.7
(-0.8) 		218.9
(-3) 		383
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193593_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.120
Sequence Type Exemplar sequence
Representative Public ID M01D7.7 NCBI
Target Description M01D7.7 /REP_DB=WormBase Gene ID /WP=CE12272 /GEN=egl-30 /TR=Q17386 /GB=AAB58071.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170806(11), NM_001026403(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170806 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-30) (egl-30) mRNA, complete cds. 11/11 A 
NM_001026403 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-30) (egl-30) mRNA, complete cds. 11/11 None
GENEFINDER00000009603 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1836822:1840969:1 10/11 None
SNAP00000009587 ENSEMBL cdna:SNAP chromosome:CEL140:I:1838044:1840889:1 11/11 None
M01D7.7a ENSEMBL cdna:known chromosome:CEL140:I:1835680:1841924:1 gene:M01D7.7 11/11 None
M01D7.7b ENSEMBL cdna:known chromosome:CEL140:I:1837772:1840889:1 gene:M01D7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1835936-1840889(+) 100.0 100.0
Public Domain and Genome References
Ensembl M01D7.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB58071 Egg laying defective protein 30, isoform a [Caenorhabditis elegans] ref|NP_740789.1| EGg Laying defective family member (egl-30) [Caenorhabditis elegans] gb|AAG32092.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] gb|AAB04059.1| EGL-30 0.0
blast AAW02891 egl-30 [Caenorhabditis briggsae] emb|CAE74465.1| Hypothetical protein CBG22211 [Caenorhabditis briggsae] 0.0
blast AAW02891 egl-30 [Caenorhabditis briggsae] emb|CAE74465.1| Hypothetical protein CBG22211 [Caenorhabditis briggsae] 1.0E-178
blast AAM15593 Egg laying defective protein 30, isoform b [Caenorhabditis elegans] ref|NP_001021574.1| EGg Laying defective family member (egl-30) [Caenorhabditis elegans] 1.0E-174
blast AAB58071 Egg laying defective protein 30, isoform a [Caenorhabditis elegans] ref|NP_740789.1| EGg Laying defective family member (egl-30) [Caenorhabditis elegans] gb|AAG32092.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] gb|AAB04059.1| EGL-30 1.0E-174
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193593_S_AT
ttcagccaaatactatctctccgatctccgacggctggcggtgccagactatctgccaac
cgagcaggacattctgcgtgttcgtgtgccaaccactggtatcattgaatatccatttga
tttggagcagatcatctttcgaatggtggacgtcggaggtcagcgatcagaaaggcggaa
gtggatccactgtttcgaaaatgtcacctcaatcatgttcctggtggcgctttccgagta
tgatcaggtgttggtcgagtgtgacaacgagaaccgaatggaagaatcgaaagctctgtt
ccgaacgatcatcacgtacccatggttcaccaactcatcggtcattctattcctgaacaa
gaaggatctgctcgaggagaagattctgtactcgcatctcgctgactactttcccgaata
tgacggacccccacgcgatccgatcgccgcccgcgagtttattctcaaaatgtttgtcga
cttgaatccggacgccgacaagattatctactctcattttacgtgcgcgactgatacgga
aaacattcggttcgtgttcgccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAGCCAAATACTATCTCTCCGAT 59 687 465 Antisense
ATGTCACCTCAATCATGTTCCTGGT 185 49 665 Antisense
TGGTGGCGCTTTCCGAGTATGATCA 581 553 686 Antisense
GAACGATCATCACGTACCCATGGTT 327 347 767 Antisense
ACCCATGGTTCACCAACTCATCGGT 29 91 782 Antisense
AACTCATCGGTCATTCTATTCCTGA 520 139 796 Antisense
AGAAGATTCTGTACTCGCATCTCGC 329 75 842 Antisense
CGCTGACTACTTTCCCGAATATGAC 618 255 864 Antisense
GTTTGTCGACTTGAATCCGGACGCC 158 449 936 Antisense
TTATCTACTCTCATTTTACGTGCGC 173 681 968 Antisense
GAAAACATTCGGTTCGTGTTCGCCG 432 355 1003 Antisense
Sequence Source Affymetrix Proprietary Database