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[193591_s_at] NADH-ubiquinone oxidoreductase 20 KD subunit

Description W10D5.2 /REP_DB=WormBase Gene ID /WP=CE14780 /TR=SW:Q94360 /GB=CAB02132.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase 20 KD subunit
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 13      6grp_total > 32      4grp_total > 01      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4516
1 2 3 4 range
A 3090.6
(-2)
P 		2491.4
(-3)
P 		3038.3
(-2)
P 		5932.2
(3)
P 		3441
B 2656.6
(-2.7)
P 		2425.7
(-3)
P 		2662.8
(-2.7)
P 		6941.6
(3)
P 		4516
C 2549.2
(-3)
P 		2591.5
(-2.9)
P 		2973.5
(-2.3)
P 		6191.8
(3)
P 		3643
avr 2765.5
(-2.6) 		2502.9
(-3) 		2891.5
(-2.4) 		6355.2
(3) 		3852

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2037
1 2 3 4 5 6 range
A 2437.7
(-0.6)
P 		3301.1
(3)
P 		2674.6
(0.4)
P 		3085.6
(2.1)
P 		1879
(-3)
P 		2229.4
(-1.5)
P 		1422
B 1984.9
(-1.6)
P 		2791.9
(2.6)
P 		2870.8
(3)
P 		2653.9
(1.9)
P 		2139.4
(-0.8)
P 		1725.2
(-3)
P 		1146
C 3762.2
(3)
P 		2405
(-1.3)
P 		3402.9
(1.9)
P 		2303.4
(-1.6)
P 		2649.2
(-0.5)
P 		1867.4
(-3)
P 		1895
avr 2728.3
(1.5) 		2832.7
(2.1) 		2982.8
(3) 		2681
(1.3) 		2222.5
(-1.4) 		1940.7
(-3) 		1042
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 939
1 2 3 4 range
A 1125.3
(3)
P 		540.8
(-3)
P 		794.3
(-0.4)
P 		868.4
(0.4)
P 		585
B 847.2
(-1.3)
P 		599.1
(-3)
P 		617
(-2.9)
P 		1479.3
(3)
P 		880
C 843.9
(-1)
P 		745.1
(-3)
P 		1036.3
(2.8)
P 		1044
(3)
P 		299
avr 938.8
(0.7) 		628.3
(-3) 		815.9
(-0.8) 		1130.6
(3) 		502

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 945
1 2 3 4 5 6 range
A 449.4
(-3)
P 		1174
(3)
P 		582.5
(-1.9)
P 		782.6
(-0.2)
P 		567
(-2)
P 		542.8
(-2.2)
P 		725
B 690.7
(-2)
P 		929.3
(3)
P 		697.1
(-1.9)
P 		702.9
(-1.7)
P 		869.2
(1.7)
P 		643.3
(-3)
P 		286
C 1394.5
(3)
P 		733.9
(-1.3)
P 		866.1
(-0.4)
P 		463.2
(-3)
P 		857.2
(-0.5)
P 		625.1
(-2)
P 		931
avr 844.9
(1.2) 		945.7
(3) 		715.2
(-1) 		649.6
(-2.2) 		764.5
(-0.2) 		603.7
(-3) 		342
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193591_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1421
Sequence Type Exemplar sequence
Representative Public ID W10D5.2 NCBI
Target Description W10D5.2 /REP_DB=WormBase Gene ID /WP=CE14780 /TR=SW:Q94360 /GB=CAB02132.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase 20 KD subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060044(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060044 NCBI Caenorhabditis elegans W10D5.2 (W10D5.2) mRNA, complete cds. 11/11 None
GENEFINDER00000007424 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9110350:9118731:-1 11/11 None
SNAP00000007423 ENSEMBL cdna:SNAP chromosome:CEL140:I:9116562:9118731:-1 11/11 None
W10D5.2.2 ENSEMBL cdna:known chromosome:CEL140:I:9116314:9119042:-1 gene:W10D5.2 11/11 None
W10D5.2.3 ENSEMBL cdna:known chromosome:CEL140:I:9116314:9118733:-1 gene:W10D5.2 11/11 None
W10D5.2.1 ENSEMBL cdna:known chromosome:CEL140:I:9116314:9118731:-1 gene:W10D5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9116492-9118662(-) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-ubiquinone oxidoreductase 20 KD subunit
Ensembl W10D5.2
Entrez Gene 172734 Entrez gene
SwissProt Q94360 EMBL-EBI
WormBase CE14780 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1636029_S_AT dm
DROSGENOME1:154574_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6120 mitochondrial electron transport, NADH to ubiquinone inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8137 NADH dehydrogenase (ubiquinone) activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02133 Hypothetical protein W10D5.3c [Caenorhabditis elegans] ref|NP_492444.2| GEX Interacting protein family member (gei-17) [Caenorhabditis elegans] 0.0
blast CAD98730 Hypothetical protein W10D5.3f [Caenorhabditis elegans] ref|NP_001021678.1| GEX Interacting protein family member (gei-17) [Caenorhabditis elegans] 0.0
blast CAI79179 Hypothetical protein W10D5.3g [Caenorhabditis elegans] ref|NP_001021679.1| GEX Interacting protein family member (gei-17) [Caenorhabditis elegans] 0.0
blast CAB02132 Hypothetical protein W10D5.2 [Caenorhabditis elegans] ref|NP_492445.1| W10D5.2 [Caenorhabditis elegans] sp|Q94360|NUKM_CAEEL Probable NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor (Complex I-20KD) (CI-20KD) 1.0E-112
blast CAE67039 Hypothetical protein CBG12445 [Caenorhabditis briggsae] 1.0E-110
Protein Domains
Database ID Description E-Value
Pfam IPR006137 EMBL-EBI NADH ubiquinone oxidoreductase domain, 20 kDa subunit 4.5E-64
Pfam IPR004181 EMBL-EBI Zn-finger, MIZ type 4.6E-43
Pfam IPR006137 EMBL-EBI NADH ubiquinone oxidoreductase domain, 20 kDa subunit 6.2E-58
Sequence
Target Sequence 
>C. ELEGANS:193591_S_AT
gctttcggcacttcgaactgccggggcagtcggtactcgccgtcttgcttcgactcaagc
gatcgccagtaacagcgaggcaccaaagggtattgccaccacaggaactccattcctgaa
tccatcgtctaaagccgagtacgcgttggctcgtcttgatgatgtcctgaatttggctca
aagaggttcaatttggccactgacgttcggtctcgcatgttgtgctgtcgaaatgatgca
cttcgctgcgccaagatatgatatggatcggtacggagtcgttttcagagcctctcccag
acaggccgatttgattttcgttgccggtacagtaaccaacaaaatggcacctgcacttcg
ccgcatatatgatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTTCGGCACTTCGAACTGCCGGG 35 303 15 Antisense
GTCTTGCTTCGACTCAAGCGATCGC 112 469 56 Antisense
CCATTCCTGAATCCATCGTCTAAAG 62 271 124 Antisense
AAAGCCGAGTACGCGTTGGCTCGTC 455 123 145 Antisense
TTGGCTCGTCTTGATGATGTCCTGA 709 705 160 Antisense
GAGGTTCAATTTGGCCACTGACGTT 531 401 197 Antisense
GGTCTCGCATGTTGTGCTGTCGAAA 99 503 223 Antisense
ATGCACTTCGCTGCGCCAAGATATG 205 43 250 Antisense
TACGGAGTCGTTTTCAGAGCCTCTC 528 641 286 Antisense
TCTCCCAGACAGGCCGATTTGATTT 324 611 307 Antisense
CCTGCACTTCGCCGCATATATGATC 579 265 364 Antisense
Sequence Source Affymetrix Proprietary Database