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[193587_at] guanine nucleotide-binding protein

Description C55H1.2 /REP_DB=WormBase Gene ID /WP=CE27889 /GEN=gpa-10 /TR=Q18873 /GB=AAA97988.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_total > 16      4grp_total > 14      6grp_cgc > 05      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 72.2
(3)
P 		20.6
(-3)
A 		24.2
(-2.6)
A 		68.1
(2.5)
M 		52
B 49.8
(0.7)
A 		52.8
(1)
A 		18.5
(-3)
A 		69.7
(3)
P 		51
C 62.3
(0.2)
P 		12.4
(-3)
A 		34.2
(-1.6)
A 		105.1
(3)
P 		93
avr 61.4
(0.9) 		28.6
(-2.7) 		25.6
(-3) 		81
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 69.1
(3)
P 		52.9
(1.5)
P 		46.8
(0.9)
P 		5.4
(-3)
A 		36.9
(-0)
A 		35.4
(-0.2)
A 		64
B 81.5
(3)
P 		57.2
(0.6)
A 		56.6
(0.5)
P 		21
(-3)
A 		38.6
(-1.3)
A 		31.1
(-2)
A 		61
C 55.6
(3)
P 		52.6
(2.4)
P 		27.9
(-3)
A 		29.3
(-2.7)
A 		32.7
(-2)
P 		36
(-1.2)
A 		28
avr 68.7
(3) 		54.2
(1.3) 		43.8
(0) 		18.6
(-3) 		36.1
(-0.9) 		34.2
(-1.1) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 26.3
(3)
P 		4.5
(-3)
A 		6.3
(-2.5)
A 		10
(-1.5)
M 		22
B 15.9
(3)
A 		13
(1.5)
A 		4.3
(-3)
A 		14.8
(2.4)
P 		12
C 20.6
(3)
P 		3.6
(-3)
A 		11.9
(-0.1)
A 		17.7
(2)
P 		17
avr 20.9
(3) 		7
(-3) 		7.5
(-2.8) 		14.2
(0.1) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 12.7
(0.9)
P 		18.8
(3)
P 		10.2
(0)
P 		1.4
(-3)
A 		11.1
(0.3)
A 		8.6
(-0.5)
A 		17
B 28.4
(3)
P 		19
(0.5)
A 		13.7
(-0.9)
P 		5.6
(-3)
A 		15.7
(-0.3)
A 		11.6
(-1.4)
A 		23
C 20.6
(3)
P 		16.1
(1.2)
P 		7.1
(-2.5)
A 		5.9
(-3)
A 		10.6
(-1.1)
P 		12
(-0.5)
A 		15
avr 20.6
(3) 		18
(2) 		10.3
(-0.8) 		4.3
(-3) 		12.5
(0) 		10.7
(-0.6) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193587_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12226
Sequence Type Exemplar sequence
Representative Public ID C55H1.2 NCBI
Target Description C55H1.2 /REP_DB=WormBase Gene ID /WP=CE27889 /GEN=gpa-10 /TR=Q18873 /GB=AAA97988.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072344(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072344 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-10) (gpa-10) mRNA, complete cds. 10/11 None
SNAP00000025884 ENSEMBL cdna:SNAP chromosome:CEL140:V:6855318:6857397:-1 10/11 None
GENEFINDER00000025886 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6855318:6857403:-1 10/11 None
C55H1.2 ENSEMBL cdna:known chromosome:CEL140:V:6855318:6857397:-1 gene:C55H1.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6855318-6857398(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-10
Ensembl C55H1.2
Entrez Gene 179075 Entrez gene
WormBase CE27889 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA97988 G protein, alpha subunit protein 10 [Caenorhabditis elegans] ref|NP_504745.1| G Protein, Alpha subunit family member (gpa-10) [Caenorhabditis elegans] gb|AAG32085.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q9BIG4|GPA10_CAEEL Guanine nucleotide-binding protein alpha-10 subunit 0.0
blast T29826 hypothetical protein C55H1.2 - Caenorhabditis elegans 0.0
blast AAW02894 gpa-10 [Caenorhabditis briggsae] sp|Q4VT42|GPA10_CAEBR Guanine nucleotide-binding protein alpha-10 subunit 1.0E-137
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 8.8E-114
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 8.8E-114
Sequence
Target Sequence 
>C. ELEGANS:193587_AT
atgtcacacatttcttcgacaacatcgaccgtataaaattgccaggctttgaaccgacaa
atcaggatattgttcacatacgggtacctacaacgggagttgcacaagtcgaagttatta
tgaacaatatcaaattgatgatttgcgattgtggtggacaacgatctgaaagacgaaaat
ggtatcactttttcgatgaggctgacgctgttctttttgtagcggctattagcgagtttg
atcagaagttagcggaggatgaagagacgaaccgtatgcatgaatccatcagattgttct
ggacagtttttaatggaaagtttttcaaaaaagcggcagtcatacttttcctgaacaaaa
ttgatttgttcgaggagaaagtaaaacatgtgaagattaaggattactttccaaaatttg
aaggagcaaacacagtttcagaaggtacaaagttctttcggagacaatttagagaaggga
ttcacccggacttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCACACATTTCTTCGACAACAT 221 49 548 Antisense
AAATTGCCAGGCTTTGAACCGACAA 470 113 583 Antisense
GTTCACATACGGGTACCTACAACGG 676 443 619 Antisense
ACAACGGGAGTTGCACAAGTCGAAG 287 113 637 Antisense
ATGGTATCACTTTTTCGATGAGGCT 230 51 726 Antisense
ATGAGGCTGACGCTGTTCTTTTTGT 549 45 743 Antisense
GCGGCTATTAGCGAGTTTGATCAGA 579 293 769 Antisense
GAGACGAACCGTATGCATGAATCCA 705 393 811 Antisense
GAATCCATCAGATTGTTCTGGACAG 593 329 829 Antisense
AAGCGGCAGTCATACTTTTCCTGAA 159 151 878 Antisense
AGAGAAGGGATTCACCCGGACTTCA 384 39 1018 Antisense
Sequence Source Affymetrix Proprietary Database