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[193568_at] guaninie nucleotide-binding protein

Description F53B1.7 /REP_DB=WormBase Gene ID /WP=CE04647 /GEN=gpa-5 /TR=Q20701 /GB=AAB52648.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guaninie nucleotide-binding protein
Cluster 6grp_total > 46      4grp_total > 02      6grp_cgc > 17      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 15.7
(-3)
A 		27.4
(-2)
A 		19.7
(-2.7)
A 		84.8
(3)
A 		69
B 25.5
(-1.7)
A 		43.3
(3)
A 		33.9
(0.5)
A 		20.8
(-3)
A 		23
C 60.6
(3)
A 		29.7
(-3)
A 		46.9
(0.3)
A 		47
(0.4)
A 		31
avr 33.9
(-2.9) 		33.5
(-3) 		33.5
(-3) 		50.9
(3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 20.8
(-1.5)
A 		15.9
(-2.1)
A 		8.3
(-3)
A 		20.4
(-1.6)
A 		16.7
(-2)
A 		58.6
(3)
A 		50
B 28.8
(2.3)
A 		30.1
(2.6)
A 		32.4
(3)
A 		1.5
(-3)
A 		22.1
(1)
A 		9.7
(-1.4)
A 		31
C 34.6
(2.3)
A 		38.8
(3)
A 		18.4
(-0.4)
A 		2.3
(-3)
A 		10.3
(-1.7)
A 		24.8
(0.7)
A 		37
avr 28.1
(2.2) 		28.3
(2.3) 		19.7
(0) 		8.1
(-3) 		16.4
(-0.8) 		31
(3) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 5.7
(-2.6)
A 		6
(-2.3)
A 		5.2
(-3)
A 		12.4
(3)
A 		7
B 8.1
(0.5)
A 		10.7
(3)
A 		7.9
(0.3)
A 		4.4
(-3)
A 		6
C 20.1
(3)
A 		8.5
(-2.7)
A 		16.3
(1.1)
A 		7.9
(-3)
A 		12
avr 11.3
(3) 		8.4
(-2.6) 		9.8
(0.1) 		8.2
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 3.8
(-2)
A 		5.7
(-1.1)
A 		1.8
(-3)
A 		5.2
(-1.4)
A 		5
(-1.5)
A 		14.3
(3)
A 		13
B 10
(3)
A 		10
(3)
A 		7.9
(1.7)
A 		0.4
(-3)
A 		9
(2.4)
A 		3.6
(-1)
A 		10
C 12.8
(3)
A 		11.8
(2.5)
A 		4.7
(-1)
A 		0.5
(-3)
A 		3.3
(-1.6)
A 		8.3
(0.8)
A 		12
avr 8.9
(2.8) 		9.2
(3) 		4.8
(-0.7) 		2
(-3) 		5.8
(0.2) 		8.7
(2.6) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193568_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18431
Sequence Type Exemplar sequence
Representative Public ID F53B1.7 NCBI
Target Description F53B1.7 /REP_DB=WormBase Gene ID /WP=CE04647 /GEN=gpa-5 /TR=Q20701 /GB=AAB52648.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guaninie nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075992(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075992 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-5) (gpa-5) mRNA, complete cds. 11/11 None
SNAP00000022994 ENSEMBL cdna:SNAP chromosome:CEL140:X:2118888:2121055:-1 11/11 None
GENEFINDER00000022998 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2118888:2121055:-1 11/11 None
F53B1.7 ENSEMBL cdna:known chromosome:CEL140:X:2118888:2121055:-1 gene:F53B1.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2118887-2121055(-) 100.0 100.0
Public Domain and Genome References
Gene Title guaninie nucleotide-binding protein
Gene Symbol gpa-5
Ensembl F53B1.7
Entrez Gene 180527 Entrez gene
SwissProt Q20701 EMBL-EBI
WormBase CE04647 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52648 G protein, alpha subunit protein 5 [Caenorhabditis elegans] ref|NP_508393.1| G Protein, Alpha subunit family member (gpa-5) [Caenorhabditis elegans] gb|AAG32081.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q20701|GPA5_CAEEL Guanine nucleotide-binding protein alpha-5 subunit 0.0
blast AAW02904 gpa-5 [Caenorhabditis briggsae] sp|Q619V5|GPA5_CAEBR Guanine nucleotide-binding protein alpha-5 subunit emb|CAE68349.1| Hypothetical protein CBG14082 [Caenorhabditis briggsae] 1.0E-155
blast CAA94612 Hypothetical protein R10H10.5 [Caenorhabditis elegans] ref|NP_501921.1| G Protein, Alpha subunit family member (gpa-7) [Caenorhabditis elegans] gb|AAG32083.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q21917|GPA7_CAEEL Guanine nucleotide-binding protein alpha-7 subunit 1.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 7.5E-21
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.8E-29
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 2.4E-22
Sequence
Target Sequence 
>C. ELEGANS:193568_AT
acagactactcttggcgttcatgaaatttcatttgactacacgaaacacattattcgtct
tattgacgtgggtggccaaaaaaccgagcgtcgcaaatggatccacttcttcgaaggtgt
cacggcggtgatgttcgtgtgctctctggcatctttcaatcaaacaacagaggaagagcc
aaaggcgtttgtatgggaatcgtcattgaacaaagtgcaaaacaaggtgctcgttcggtc
tgctggaaaggcaaaagttgagaaaccagggttgatcaaccgattggatgaaagtgtaga
cttgttcaaatcgattcgtgagaatagcttcctcaaaatgtcgaatttcatgttgtttct
gaataaaaaggatcttctgaccaaaaaactcacaaaagtcgtgttttccgactacttccc
cgactacaaaaagtggattaccaatgacaattcggacgtttctgtcgcggagtttatcga
aaacatgttccgtgagggtcttgagccggagaagcggatgtacgcgcatttgactcaagc
gacagtaactgccaatatcgaaggaacgtttgcattgtgctgtgacgtcatctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGACTACTCTTGGCGTTCATGAA 249 107 543 Antisense
GTGGCCAAAAAACCGAGCGTCGCAA 11 493 614 Antisense
GAGCGTCGCAAATGGATCCACTTCT 260 387 628 Antisense
GGATCCACTTCTTCGAAGGTGTCAC 543 511 641 Antisense
GTCACGGCGGTGATGTTCGTGTGCT 114 465 661 Antisense
GCTCTCTGGCATCTTTCAATCAAAC 704 299 683 Antisense
CAAAACAAGGTGCTCGTTCGGTCTG 360 189 760 Antisense
AATTCGGACGTTTCTGTCGCGGAGT 505 177 991 Antisense
ACATGTTCCGTGAGGGTCTTGAGCC 259 107 1025 Antisense
GGATGTACGCGCATTTGACTCAAGC 1 515 1058 Antisense
TTGCATTGTGCTGTGACGTCATCTT 478 703 1112 Antisense
Sequence Source Affymetrix Proprietary Database