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[193565_at] guanine nucleotide-binding protein

Description R10H10.5 /REP_DB=WormBase Gene ID /WP=CE06296 /GEN=gpa-7 /TR=Q21917 /GB=CAA94612.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_total > 07      4grp_total > 39      6grp_cgc > 24      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 223.9
(3)
P 		120.4
(-1.2)
A 		76.6
(-3)
A 		173.5
(0.9)
M 		147
B 186.3
(3)
P 		102.9
(-0.3)
M 		79.1
(-1.2)
A 		34.5
(-3)
A 		152
C 234
(3)
P 		89.8
(-3)
A 		105.9
(-2.3)
A 		178.3
(0.7)
A 		144
avr 214.7
(3) 		104.4
(-2.2) 		87.2
(-3) 		128.8
(-1) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 133.3
(3)
P 		88.8
(-0.2)
P 		104.1
(0.9)
P 		108.2
(1.2)
P 		51
(-3)
A 		73.6
(-1.4)
A 		82
B 152.3
(3)
P 		133
(1.6)
P 		72.4
(-2.7)
A 		88.8
(-1.6)
A 		77.7
(-2.4)
A 		68.8
(-3)
A 		84
C 209.4
(3)
P 		172.1
(1.6)
P 		121.2
(-0.4)
P 		77.8
(-2)
A 		52.8
(-3)
A 		90.6
(-1.6)
A 		157
avr 165
(3) 		131.3
(1.1) 		99.2
(-0.8) 		91.6
(-1.2) 		60.5
(-3) 		77.7
(-2) 		105
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 81.5
(3)
P 		26.1
(-2.4)
A 		20
(-3)
A 		25.4
(-2.5)
M 		62
B 59.4
(3)
P 		25.4
(-0.9)
M 		18.3
(-1.7)
A 		7.4
(-3)
A 		52
C 77.5
(3)
P 		25.8
(-3)
A 		36.9
(-1.7)
A 		30.1
(-2.5)
A 		52
avr 72.8
(3) 		25.8
(-2.4) 		25.1
(-2.5) 		21
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 24.6
(0.4)
P 		31.6
(3)
P 		22.7
(-0.3)
P 		27.4
(1.4)
P 		15.4
(-3)
A 		17.9
(-2.1)
A 		16
B 53
(3)
P 		44.3
(1.5)
P 		17.6
(-3)
A 		23.5
(-2)
A 		31.6
(-0.6)
A 		25.6
(-1.6)
A 		35
C 77.6
(3)
P 		52.5
(0.6)
P 		30.8
(-1.5)
P 		15.6
(-3)
A 		17.1
(-2.9)
A 		30.3
(-1.6)
A 		62
avr 51.7
(3) 		42.8
(1.2) 		23.7
(-2.5) 		22.2
(-2.8) 		21.4
(-3) 		24.6
(-2.4) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193565_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8462
Sequence Type Exemplar sequence
Representative Public ID R10H10.5 NCBI
Target Description R10H10.5 /REP_DB=WormBase Gene ID /WP=CE06296 /GEN=gpa-7 /TR=Q21917 /GB=CAA94612.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069520(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069520 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-7) (gpa-7) mRNA, complete cds. 11/11 None
SNAP00000020334 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10405987:10408238:1 11/11 None
GENEFINDER00000020338 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10403991:10408238:1 11/11 None
R10H10.5 ENSEMBL cdna:known chromosome:CEL140:IV:10403991:10408238:1 gene:R10H10.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10403996-10408244(+) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-7
Ensembl R10H10.5
Entrez Gene 177931 Entrez gene
SwissProt Q21917 EMBL-EBI
WormBase CE06296 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21115.1.S1_AT similar to Guanine nucleotide-binding protein G(z), alpha subunit (G(x) alpha chain) (Gz-alpha) cfa
HG-U133_PLUS_2:204993_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HG-FOCUS:204993_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HG-U133A:204993_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HG-U133A_2:204993_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HU35KSUBA:D90150_RNA1_S_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HU35KSUBC:RC_AA281291_S_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HUGENEFL:J03260_S_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
U133_X3P:G4504050_3P_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HG-U95AV2:38279_AT guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HG-U133_PLUS_2:229977_AT Guanine nucleotide binding protein (G protein), alpha z polypeptide hs
HG-U133B:229977_AT Guanine nucleotide binding protein (G protein), alpha z polypeptide hs
MOUSE430A_2:1426517_AT guanine nucleotide binding protein, alpha z subunit mm
MOE430A:1426517_AT guanine nucleotide binding protein, alpha z subunit mm
MOUSE430_2:1426517_AT guanine nucleotide binding protein, alpha z subunit mm
MU11KSUBB:MSA.27268.0_S_AT guanine nucleotide binding protein, alpha z subunit mm
MG-U74AV2:100386_AT guanine nucleotide binding protein, alpha z subunit mm
MG-U74BV2:106545_AT guanine nucleotide binding protein, alpha z subunit mm
MG-U74BV2:163413_AT guanine nucleotide binding protein, alpha z subunit mm
RG-U34A:J03773_S_AT guanine nucleotide binding protein, alpha z subunit rn
RG-U34A:U77483MRNA_S_AT guanine nucleotide binding protein, alpha z subunit rn
RAE230A:1387095_AT guanine nucleotide binding protein, alpha z subunit rn
RAT230_2:1387095_AT guanine nucleotide binding protein, alpha z subunit rn
RAE230A:1368185_AT guanine nucleotide binding protein, alpha z subunit rn
RAT230_2:1368185_AT guanine nucleotide binding protein, alpha z subunit rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94612 Hypothetical protein R10H10.5 [Caenorhabditis elegans] ref|NP_501921.1| G Protein, Alpha subunit family member (gpa-7) [Caenorhabditis elegans] gb|AAG32083.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q21917|GPA7_CAEEL Guanine nucleotide-binding protein alpha-7 subunit 0.0
blast AAW02906 gpa-7 [Caenorhabditis briggsae] sp|Q61MQ8|GPA7_CAEBR Guanine nucleotide-binding protein alpha-7 subunit emb|CAE63826.1| Hypothetical protein CBG08377 [Caenorhabditis briggsae] 0.0
blast CAA94612 Hypothetical protein R10H10.5 [Caenorhabditis elegans] ref|NP_501921.1| G Protein, Alpha subunit family member (gpa-7) [Caenorhabditis elegans] gb|AAG32083.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q21917|GPA7_CAEEL Guanine nucleotide-binding protein alpha-7 subunit 1.0E-176
blast AAW02906 gpa-7 [Caenorhabditis briggsae] sp|Q61MQ8|GPA7_CAEBR Guanine nucleotide-binding protein alpha-7 subunit emb|CAE63826.1| Hypothetical protein CBG08377 [Caenorhabditis briggsae] 1.0E-174
blast EAL26334 GA15300-PA [Drosophila pseudoobscura] 1.0E-119
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193565_AT
tgatgactctgcaaaatacttccttgacaacttgccacgtctctcatctccaaactacgt
gccaagtgagcaggatcttttgaggacacgaataaaaacaacaggaataacagaagtttt
gtttgaactgaaagggctcacatttcgagtgatagatgttggagggcaacgatcagaaag
aaagaaatggattcattgtttcgataatgttaatgcaatcatttttatatcatctttatc
agagtatgatcaaactttgagagaagataattgcacgaaccgaatgcaagaatcattgaa
actgtttgactcaatctgtaatagtccatggtttgcggatattcactttattttattttt
aaataagaaggacttgttcgctgaaaaaattgtccgatcaccattgaccgtttgcttccc
agaatacaagggacaacagaatcaaacagaatgtattaattacattcaatggaagtttga
acaacttaataggtcctcccaacgtgaaatctattgtcatcacacatgtgccacagacac
aaataacgttcaattcgtgttggacgcctgtctcgatatgattat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATGACTCTGCAAAATACTTCCTT 21 567 453 Antisense
AAATACTTCCTTGACAACTTGCCAC 540 115 466 Antisense
TCATCTCCAAACTACGTGCCAAGTG 331 621 496 Antisense
GAGCAGGATCTTTTGAGGACACGAA 577 389 520 Antisense
AATTGTCCGATCACCATTGACCGTT 631 173 840 Antisense
ACCGTTTGCTTCCCAGAATACAAGG 624 85 859 Antisense
TGAACAACTTAATAGGTCCTCCCAA 534 571 930 Antisense
TAGGTCCTCCCAACGTGAAATCTAT 499 649 942 Antisense
TTGTCATCACACATGTGCCACAGAC 178 705 966 Antisense
ACGTTCAATTCGTGTTGGACGCCTG 216 95 998 Antisense
TGGACGCCTGTCTCGATATGATTAT 99 549 1013 Antisense
Sequence Source Affymetrix Proprietary Database