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[193562_s_at]

Description F23B2.5 /REP_DB=WormBase Gene ID /WP=CE09585 /GEN=flp-1 /TR=SW:P41855 /GB=CAB05179.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=FMFRamide neuropeptide precursor
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 53      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 37      6grp_total > 30      4grp_total > 12      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11900
1 2 3 4 range
A 5785.7
(-0.9)
P 		2990.2
(-2.5)
P 		2085.5
(-3)
P 		12588.3
(3)
P 		10503
B 5279.7
(-1)
P 		3203.3
(-2.2)
P 		1824.4
(-3)
P 		12289.8
(3)
P 		10465
C 5295.5
(-1.4)
P 		3206.3
(-2.5)
P 		2174.2
(-3)
P 		13724.3
(3)
P 		11550
avr 5453.6
(-1.1) 		3133.3
(-2.4) 		2028
(-3) 		12867.5
(3) 		10840

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3321
1 2 3 4 5 6 range
A 4825.2
(-0.9)
P 		5439.6
(0.2)
P 		7001.8
(3)
P 		6158.3
(1.5)
P 		3681
(-3)
P 		4465.4
(-1.6)
P 		3321
B 4126
(-3)
P 		6279.4
(3)
P 		5807.8
(1.7)
P 		4700.2
(-1.4)
P 		4605.4
(-1.7)
P 		4582.5
(-1.7)
P 		2153
C 4871.2
(-0.7)
P 		5445.1
(2.6)
P 		5103.6
(0.7)
P 		5508
(3)
P 		4470.5
(-3)
P 		5482.2
(2.9)
P 		1038
avr 4607.5
(-1.8) 		5721.4
(2.1) 		5971.1
(3) 		5455.5
(1.2) 		4252.3
(-3) 		4843.4
(-0.9) 		1719
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2196
1 2 3 4 range
A 2106.6
(3)
P 		649.1
(-2.6)
P 		545.2
(-3)
P 		1842.7
(2)
P 		1561
B 1683.7
(0.4)
P 		791.1
(-2)
P 		422.8
(-3)
P 		2619
(3)
P 		2196
C 1753
(0.8)
P 		921.8
(-2.4)
P 		757.8
(-3)
P 		2314.1
(3)
P 		1556
avr 1847.8
(1.5) 		787.3
(-2.2) 		575.3
(-3) 		2258.6
(3) 		1683

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1201
1 2 3 4 5 6 range
A 889.5
(-3)
P 		1934.6
(3)
P 		1524.8
(0.6)
P 		1561.9
(0.9)
P 		1110.8
(-1.7)
P 		1087.2
(-1.9)
P 		1045
B 1435.7
(-1.6)
P 		2090.1
(3)
P 		1410.3
(-1.8)
P 		1244.9
(-3)
P 		1871.1
(1.4)
P 		1708.8
(0.3)
P 		845
C 1805.5
(2.8)
P 		1661.5
(1.6)
P 		1299
(-1.4)
P 		1107.7
(-3)
P 		1446.6
(-0.2)
P 		1835.2
(3)
P 		728
avr 1376.9
(-2.3) 		1895.4
(3) 		1411.4
(-1.9) 		1304.8
(-3) 		1476.2
(-1.3) 		1543.7
(-0.6) 		591
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193562_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10094
Sequence Type Exemplar sequence
Representative Public ID F23B2.5 NCBI
Target Description F23B2.5 /REP_DB=WormBase Gene ID /WP=CE09585 /GEN=flp-1 /TR=SW:P41855 /GB=CAB05179.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=FMFRamide neuropeptide precursor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182277(11), NM_069191(11), NM_182278(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182277 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-1) (flp-1) mRNA, complete cds. 11/11 A 
NM_069191 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-1) (flp-1) mRNA, complete cds. 11/11 A 
NM_182278 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-1) (flp-1) mRNA, complete cds. 11/11 None
SNAP00000024643 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9144694:9145958:1 11/11 None
GENEFINDER00000024651 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9144694:9146013:1 10/11 None
F23B2.5a ENSEMBL cdna:known chromosome:CEL140:IV:9144686:9146096:1 gene:F23B2.5 11/11 A 
F23B2.5b ENSEMBL cdna:known chromosome:CEL140:IV:9144686:9146116:1 gene:F23B2.5 11/11 A 
F23B2.5c ENSEMBL cdna:known chromosome:CEL140:IV:9144694:9145958:1 gene:F23B2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9144699-9145964(+) 100.0 100.0
Public Domain and Genome References
Ensembl F23B2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05179 Hypothetical protein F23B2.5a [Caenorhabditis elegans] ref|NP_501592.1| FMRF-Like Peptide family member (flp-1) [Caenorhabditis elegans] gb|AAB22368.1| FMRFamide-like peptide [Caenorhabditis elegans] gb|AAC46464.1| FLP-1 [Caenorhabditis elegans] sp|P41855|FARP_CAEEL FMRFamide-like neuropeptides precursor [Contains: PNFMRY-amide; AGSDPNFLRF-amide; SQPNFLRF-amide; ASGDPNFLRF-amide; SDPNFLRF-amide (PF1); AAADPNFLRF-amide; SADPNFLRF-amide (PF2); PNFLRF-amide] 7.0E-92
blast AAA74036 CV-FLP-1B 2.0E-88
blast CAE74119 Hypothetical protein CBG21782 [Caenorhabditis briggsae] 1.0E-87
blast CAD56243 Hypothetical protein F23B2.5b [Caenorhabditis elegans] ref|NP_872077.1| FMRF-Like Peptide family member (flp-1) [Caenorhabditis elegans] 5.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0063
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0059
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.26
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0053
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.082
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0096
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0022
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0041
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0022
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.26
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0053
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.082
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0096
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0022
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0041
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.082
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0096
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0022
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0041
Sequence
Target Sequence 
>C. ELEGANS:193562_S_AT
gtgaccctaacttcttgagatttggaaaagccgctgctgatccaaatttcttgagattcg
gaaaacgatctgcagatcctaacttcttgagattcggacgctcattcgacaacttcgatc
gtgaatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGACCCTAACTTCTTGAGATTTGG 587 481 380 Antisense
TGAGATTTGGAAAAGCCGCTGCTGA 521 551 395 Antisense
AAAAGCCGCTGCTGATCCAAATTTC 244 131 405 Antisense
CCGCTGCTGATCCAAATTTCTTGAG 369 263 410 Antisense
GCTGATCCAAATTTCTTGAGATTCG 600 295 415 Antisense
TTCGGAAAACGATCTGCAGATCCTA 350 695 436 Antisense
CGATCTGCAGATCCTAACTTCTTGA 140 249 445 Antisense
GCAGATCCTAACTTCTTGAGATTCG 522 311 451 Antisense
CTAACTTCTTGAGATTCGGACGCTC 71 227 458 Antisense
AGATTCGGACGCTCATTCGACAACT 478 63 469 Antisense
CATTCGACAACTTCGATCGTGAATC 578 215 482 Antisense
Sequence Source Affymetrix Proprietary Database