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[193561_at]

Description F23B2.5 /REP_DB=WormBase Gene ID /WP=CE09585 /GEN=flp-1 /TR=SW:P41855 /GB=CAB05179.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=FMFRamide neuropeptide precursor
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 53      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 23      6grp_total > 30      4grp_total > 12      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7197
1 2 3 4 range
A 2521.4
(-0.9)
P 		1268.1
(-2.3)
P 		608.9
(-3)
P 		6154.5
(3)
P 		5546
B 2286.9
(-1.1)
P 		996.2
(-2.8)
P 		842.9
(-3)
P 		5436
(3)
P 		4593
C 2672.4
(-1.3)
P 		1308.8
(-2.5)
P 		714.4
(-3)
P 		7806.2
(3)
P 		7092
avr 2493.6
(-1.1) 		1191
(-2.5) 		722.1
(-3) 		6465.6
(3) 		5744

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1787
1 2 3 4 5 6 range
A 1782.7
(-1.4)
P 		2821.2
(2.1)
P 		2593.8
(1.3)
P 		3092.2
(3)
P 		1305
(-3)
P 		2394.9
(0.7)
P 		1787
B 1615.5
(-3)
P 		2506.5
(1.7)
P 		2763.1
(3)
P 		1786.9
(-2.1)
P 		1757.7
(-2.3)
P 		1811.3
(-2)
P 		1148
C 2818.6
(3)
P 		2228.9
(-1.2)
P 		2609.7
(1.5)
P 		2218.1
(-1.3)
P 		2488.4
(0.6)
P 		1981.9
(-3)
P 		837
avr 2072.3
(-1.3) 		2518.9
(2) 		2655.5
(3) 		2365.7
(0.8) 		1850.4
(-3) 		2062.7
(-1.4) 		805
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1157
1 2 3 4 range
A 918
(3)
P 		275.3
(-2.1)
P 		159.2
(-3)
P 		900.9
(2.9)
P 		759
B 729.3
(0.3)
P 		246
(-2.7)
P 		195.3
(-3)
P 		1158.4
(3)
P 		963
C 884.6
(0.6)
P 		376.3
(-2.3)
P 		249
(-3)
P 		1316.3
(3)
P 		1067
avr 844
(1.2) 		299.2
(-2.4) 		201.2
(-3) 		1125.2
(3) 		924

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 716
1 2 3 4 5 6 range
A 328.6
(-3)
P 		1003.4
(3)
P 		564.9
(-0.9)
P 		784.2
(1.1)
P 		393.8
(-2.4)
P 		583.1
(-0.7)
P 		675
B 562.1
(-1.5)
P 		834.3
(3)
P 		671
(0.3)
P 		473.3
(-3)
P 		714.1
(1)
P 		675.4
(0.4)
P 		361
C 1044.7
(3)
P 		680.1
(-0.7)
P 		664.2
(-0.8)
P 		446.1
(-3)
P 		805.2
(0.6)
P 		663.5
(-0.8)
P 		599
avr 645.1
(-1.3) 		839.3
(3) 		633.4
(-1.6) 		567.9
(-3) 		637.7
(-1.5) 		640.7
(-1.4) 		271
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193561_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10094
Sequence Type Exemplar sequence
Representative Public ID F23B2.5 NCBI
Target Description F23B2.5 /REP_DB=WormBase Gene ID /WP=CE09585 /GEN=flp-1 /TR=SW:P41855 /GB=CAB05179.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=FMFRamide neuropeptide precursor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182277(10), NM_069191(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182277 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-1) (flp-1) mRNA, complete cds. 10/11 A 
NM_069191 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-1) (flp-1) mRNA, complete cds. 11/11 A 
SNAP00000024643 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9144694:9145958:1 11/11 None
GENEFINDER00000024651 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9144694:9146013:1 11/11 None
F23B2.5a ENSEMBL cdna:known chromosome:CEL140:IV:9144686:9146096:1 gene:F23B2.5 11/11 A 
F23B2.5b ENSEMBL cdna:known chromosome:CEL140:IV:9144686:9146116:1 gene:F23B2.5 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182278 8/11 Cross Hyb Matching Probes A B 
F23B2.5c 8/11 Cross Hyb Matching Probes A B 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177737 3 2 NM_069191 193561_at (11)
NM_182277 193561_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9144699-9145964(+) 100.0 100.0
Public Domain and Genome References
Ensembl F23B2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05179 Hypothetical protein F23B2.5a [Caenorhabditis elegans] ref|NP_501592.1| FMRF-Like Peptide family member (flp-1) [Caenorhabditis elegans] gb|AAB22368.1| FMRFamide-like peptide [Caenorhabditis elegans] gb|AAC46464.1| FLP-1 [Caenorhabditis elegans] sp|P41855|FARP_CAEEL FMRFamide-like neuropeptides precursor [Contains: PNFMRY-amide; AGSDPNFLRF-amide; SQPNFLRF-amide; ASGDPNFLRF-amide; SDPNFLRF-amide (PF1); AAADPNFLRF-amide; SADPNFLRF-amide (PF2); PNFLRF-amide] 7.0E-92
blast AAA74036 CV-FLP-1B 2.0E-88
blast CAE74119 Hypothetical protein CBG21782 [Caenorhabditis briggsae] 1.0E-87
blast CAD56243 Hypothetical protein F23B2.5b [Caenorhabditis elegans] ref|NP_872077.1| FMRF-Like Peptide family member (flp-1) [Caenorhabditis elegans] 5.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0063
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0059
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.26
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0053
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.082
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0096
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0022
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0041
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0022
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.26
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0053
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.082
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0096
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0022
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0041
Sequence
Target Sequence 
>C. ELEGANS:193561_AT
atgactctgctctaccaagtagggttattactccttgtggcagctacttataaggtgtcg
gcagaatgttgcacaccgggagccacttcagacttttgtacggtcttctcgatgctctcc
accatggagcaaaatgaagtgatgaactttataggtgaaaactgcgatggtgatgccgag
gtggcactgcaaaagatggaaaagagaaagccaaactttatgagatatgggcgatcagca
gctgtaaagtcgctgggaaagaaagccggttcagacccgaacttccttcgattcggacga
tctcaaccaaacttcttgcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGACTCTGCTCTACCAAGTAGGGT 254 45 13 Antisense
GGTTATTACTCCTTGTGGCAGCTAC 590 505 35 Antisense
GGCAGCTACTTATAAGGTGTCGGCA 82 535 51 Antisense
GTCGGCAGAATGTTGCACACCGGGA 132 473 69 Antisense
AGCCACTTCAGACTTTTGTACGGTC 3 85 93 Antisense
ACTTTTGTACGGTCTTCTCGATGCT 279 103 104 Antisense
TCGATGCTCTCCACCATGGAGCAAA 504 603 121 Antisense
ACTGCGATGGTGATGCCGAGGTGGC 136 99 173 Antisense
GAGATATGGGCGATCAGCAGCTGTA 472 395 234 Antisense
GAACTTCCTTCGATTCGGACGATCT 318 349 291 Antisense
CGATCTCAACCAAACTTCTTGCGAT 139 249 310 Antisense
Sequence Source Affymetrix Proprietary Database