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[193557_at] guanylate cyclase beta chain like

Description M04G12.3 /REP_DB=WormBase Gene ID /WP=CE12426 /GEN=gcy-34 /TR=P92006 /GB=CAB03210.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase beta chain like
Cluster 6grp_total > 90      4grp_total > 20      6grp_cgc > 96      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 57.2
(0.4)
A 		27.1
(-2.1)
A 		16.5
(-3)
A 		89.3
(3)
A 		73
B 53
(0.5)
A 		60.5
(1.1)
A 		2.8
(-3)
A 		87.7
(3)
A 		85
C 64.5
(0.1)
A 		42.5
(-2)
A 		32.6
(-3)
A 		94.4
(3)
A 		62
avr 58.2
(0.4) 		43.4
(-0.9) 		17.3
(-3) 		90.5
(3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 49.9
(-0.9)
A 		48.6
(-1.1)
A 		33.7
(-3)
A 		80.4
(3)
A 		65.8
(1.1)
A 		44.7
(-1.6)
A 		47
B 56.8
(3)
A 		41.2
(-1.4)
A 		56.3
(2.9)
A 		35.7
(-2.9)
A 		53.1
(2)
A 		35.3
(-3)
A 		22
C 59.9
(1.8)
A 		72.7
(3)
A 		16.2
(-2.5)
A 		62.7
(2)
A 		11.2
(-3)
A 		62.5
(2)
A 		62
avr 55.5
(2) 		54.2
(1.7) 		35.4
(-3) 		59.6
(3) 		43.4
(-1) 		47.5
(0) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 20.8
(3)
A 		5.9
(-2.4)
A 		4.3
(-3)
A 		13.1
(0.2)
A 		17
B 16.9
(2.4)
A 		14.9
(1.7)
A 		0.6
(-3)
A 		18.7
(3)
A 		18
C 21.3
(3)
A 		12.2
(-2.5)
A 		11.4
(-3)
A 		15.9
(-0.3)
A 		10
avr 19.7
(3) 		11
(-0.7) 		5.4
(-3) 		15.9
(1.4) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 9.2
(-2.1)
A 		17.3
(1.6)
A 		7.3
(-3)
A 		20.4
(3)
A 		19.9
(2.8)
A 		10.9
(-1.4)
A 		13
B 19.8
(2.1)
A 		13.7
(-0.9)
A 		13.7
(-0.9)
A 		9.5
(-3)
A 		21.6
(3)
A 		13.2
(-1.2)
A 		12
C 22.2
(3)
A 		22.2
(3)
A 		4.1
(-2.8)
A 		12.6
(-0.1)
A 		3.6
(-3)
A 		20.9
(2.6)
A 		19
avr 17.1
(2.6) 		17.7
(3) 		8.4
(-3) 		14.2
(0.7) 		15
(1.3) 		15
(1.3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193557_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13975
Sequence Type Exemplar sequence
Representative Public ID M04G12.3 NCBI
Target Description M04G12.3 /REP_DB=WormBase Gene ID /WP=CE12426 /GEN=gcy-34 /TR=P92006 /GB=CAB03210.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase beta chain like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073918(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073918 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-34) (gcy-34) mRNA, complete cds. 11/11 None
SNAP00000041636 ENSEMBL cdna:SNAP chromosome:CEL140:V:13385714:13392076:-1 11/11 None
GENEFINDER00000041637 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13385714:13392082:-1 11/11 None
M04G12.3 ENSEMBL cdna:known chromosome:CEL140:V:13385298:13388490:-1 gene:M04G12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13385713-13388238(-) 99.9 99.9
Public Domain and Genome References
Gene Title guanylate cyclase beta chain like
Gene Symbol gcy-34
Ensembl M04G12.3
Entrez Gene 191656 Entrez gene
SwissProt P92006 EMBL-EBI
WormBase CE12426 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604811_S_AT guanylate cyclase 1, soluble, beta 3 cfa
CANINE_2:CFA.253.1.S1_AT guanylate cyclase 1, soluble, beta 3 cfa
CANINE_2:CFAAFFX.13566.1.S1_AT guanylate cyclase 1, soluble, beta 3 cfa
DROSOPHILA_2:1635419_AT Guanylyl cyclase subunit at 100B dm
DROSGENOME1:143731_AT Guanylyl cyclase subunit at 100B dm
CHICKEN:GGA.12920.1.S1_AT guanylate cyclase 1, soluble, beta 3 gga
HG-U95AV2:37243_AT guanylate cyclase 1, soluble, beta 3 hs
HG-U133_PLUS_2:211555_S_AT guanylate cyclase 1, soluble, beta 3 hs
HG-U133A:211555_S_AT guanylate cyclase 1, soluble, beta 3 hs
HG-U133A_2:211555_S_AT guanylate cyclase 1, soluble, beta 3 hs
HUGENEFL:X66533_AT guanylate cyclase 1, soluble, beta 3 hs
U133_X3P:G2746082_3P_A_AT guanylate cyclase 1, soluble, beta 3 hs
U133_X3P:HS.77890.0.S2_3P_AT guanylate cyclase 1, soluble, beta 3 hs
HG-U133A_2:203817_AT guanylate cyclase 1, soluble, beta 3 hs
HG-FOCUS:203817_AT guanylate cyclase 1, soluble, beta 3 hs
HG-U133_PLUS_2:203817_AT guanylate cyclase 1, soluble, beta 3 hs
HG-U133A:203817_AT guanylate cyclase 1, soluble, beta 3 hs
MG-U74AV2:93954_AT guanylate cyclase 1, soluble, beta 3 mm
MG-U74BV2:163907_AT guanylate cyclase 1, soluble, beta 3 mm
MOE430A:1420871_AT guanylate cyclase 1, soluble, beta 3 mm
MOUSE430A_2:1420871_AT guanylate cyclase 1, soluble, beta 3 mm
MOUSE430_2:1420871_AT guanylate cyclase 1, soluble, beta 3 mm
MOE430A:1420872_AT guanylate cyclase 1, soluble, beta 3 mm
MOUSE430A_2:1420872_AT guanylate cyclase 1, soluble, beta 3 mm
MOUSE430_2:1420872_AT guanylate cyclase 1, soluble, beta 3 mm
MU19KSUBC:TC34994_AT guanylate cyclase 1, soluble, beta 3 mm
MU19KSUBC:TC34994_G_AT guanylate cyclase 1, soluble, beta 3 mm
RG-U34A:RC_AI233225_AT guanylate cyclase 1, soluble, beta 3 rn
RAE230A:1374389_AT guanylate cyclase 1, soluble, beta 3 rn
RAT230_2:1374389_AT guanylate cyclase 1, soluble, beta 3 rn
RAE230A:1369097_S_AT guanylate cyclase 1, soluble, beta 3 rn
RAT230_2:1369097_S_AT guanylate cyclase 1, soluble, beta 3 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03210 Hypothetical protein M04G12.3 [Caenorhabditis elegans] gb|AAT73710.1| guanylate cyclase-like protein [Caenorhabditis elegans] ref|NP_506319.1| Guanylyl CYclase family member (gcy-34) [Caenorhabditis elegans] sp|P92006|GCY34_CAEEL Soluble guanylate cyclase gcy-34 0.0
blast CAE62608 Hypothetical protein CBG06726 [Caenorhabditis briggsae] 0.0
blast AAT73709 guanylate cyclase-like protein [Caenorhabditis elegans] sp|Q6DNF7|GCY32_CAEEL Soluble guanylate cyclase gcy-32 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.1E-57
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.1E-57
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.1E-57
Sequence
Target Sequence 
>C. ELEGANS:193557_AT
gaattgtggttcttcgtggtgtttacaaagttgaaaccgtttcagacagttacatgactg
tctctggaatacctgactatacttcagagcatgcagaaaatatgtgccatgtcgcgttgg
gaatgatgtgggaagcccgttctgtgatggaccctgtaaacaagaccccgtttctgcttc
gaattggacttcattctggaactattattgccggagtagttggcactaaaatgccacgtt
attgtctattcggagagactgtaactttagcgtctcaaatggaatctctcggtgttgctg
gtaagatacaatgtagtagctggacgtattcaaaagccatggaaactggaagatttgaat
ttagtcctcgaggtagaataaatgtaaaaggacgaggagatgtggagacatattttttga
tgagaagcttgaaaaagagcatttgggagattacagatcatgaaagagatgtcaacgtga
acagcatcgagggttatgaagaattagagattttcattgaaaatgcacaaactgttaagc
atgacggacacccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTGTGGTTCTTCGTGGTGTTTA 499 323 1478 Antisense
ACATGACTGTCTCTGGAATACCTGA 553 101 1529 Antisense
ATGTGCCATGTCGCGTTGGGAATGA 142 49 1579 Antisense
GATGTGGGAAGCCCGTTCTGTGATG 257 409 1602 Antisense
GATGGACCCTGTAAACAAGACCCCG 550 403 1623 Antisense
CCCGTTTCTGCTTCGAATTGGACTT 671 259 1644 Antisense
AATGCCACGTTATTGTCTATTCGGA 468 167 1707 Antisense
ACTGTAACTTTAGCGTCTCAAATGG 437 95 1735 Antisense
GAATCTCTCGGTGTTGCTGGTAAGA 100 327 1759 Antisense
GATTTGAATTTAGTCCTCGAGGTAG 280 427 1829 Antisense
ACTGTTAAGCATGACGGACACCCGA 6 97 2008 Antisense
Sequence Source Affymetrix Proprietary Database