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[193550_s_at] NADH-ubiquinone dehydrogenase 24 KD subunit

Description F53F4.10 /REP_DB=WormBase Gene ID /WP=CE10972 /TR=SW:Q20719 /GB=CAB01203.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=NADH-ubiquinone dehydrogenase 24 KD subunit
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 51      4grp_highest_8_8 > 08      4grp_high_8_8 > 53      6grp_highest_nonorm > 26      6grp_total > 52      4grp_total > 58      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5070
1 2 3 4 range
A 6215
(-1.5)
P 		9430.1
(3)
P 		6534.6
(-1)
P 		5120
(-3)
P 		4310
B 6757.9
(0.9)
P 		6964.3
(1.2)
P 		8066.5
(3)
P 		4360.3
(-3)
P 		3706
C 5934.1
(-3)
P 		7198.7
(3)
P 		6858.6
(1.4)
P 		6071.2
(-2.3)
P 		1265
avr 6302.3
(-0.5) 		7864.4
(3) 		7153.2
(1.4) 		5183.8
(-3) 		2681

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6431
1 2 3 4 5 6 range
A 5688.1
(-1.3)
P 		7009.3
(-0.1)
P 		10332.8
(3)
P 		8077.1
(0.9)
P 		4756.3
(-2.2)
P 		3902
(-3)
P 		6431
B 4668.1
(-2.4)
P 		6815.7
(-0.1)
P 		9749.9
(3)
P 		7252.5
(0.4)
P 		5507
(-1.5)
P 		4066.6
(-3)
P 		5683
C 4466.6
(-2.9)
P 		6626.5
(0.7)
P 		7308.2
(1.8)
P 		8028
(3)
P 		4896.4
(-2.2)
P 		4416.1
(-3)
P 		3612
avr 4940.9
(-2) 		6817.2
(0.2) 		9130.3
(3) 		7785.9
(1.4) 		5053.2
(-1.9) 		4128.2
(-3) 		5002
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1641
1 2 3 4 range
A 2262.9
(3)
P 		2047
(2.1)
P 		1708.3
(0.8)
P 		749.5
(-3)
P 		1513
B 2155
(3)
P 		1720
(0.9)
P 		1869.2
(1.6)
P 		929.2
(-3)
P 		1226
C 1964.4
(1.1)
P 		2069.7
(1.6)
P 		2390.4
(3)
P 		1023.7
(-3)
P 		1367
avr 2127.4
(3) 		1945.6
(2.1) 		1989.3
(2.3) 		900.8
(-3) 		1227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1543
1 2 3 4 5 6 range
A 1048.6
(-2.6)
P 		2492.8
(3)
P 		2250.3
(2.1)
P 		2048.5
(1.3)
P 		1435.3
(-1.1)
P 		950
(-3)
P 		1543
B 1624.3
(-2.2)
P 		2268.7
(2.3)
P 		2367.6
(3)
P 		1920.9
(-0.1)
P 		2237.4
(2.1)
P 		1516.4
(-3)
P 		851
C 1655.6
(-1)
P 		2022
(3)
P 		1860.1
(1.2)
P 		1614.4
(-1.5)
P 		1584.4
(-1.8)
P 		1478.3
(-3)
P 		544
avr 1442.8
(-2.2) 		2261.2
(3) 		2159.3
(2.4) 		1861.3
(0.5) 		1752.4
(-0.2) 		1314.9
(-3) 		946
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193550_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14749
Sequence Type Exemplar sequence
Representative Public ID F53F4.10 NCBI
Target Description F53F4.10 /REP_DB=WormBase Gene ID /WP=CE10972 /TR=SW:Q20719 /GB=CAB01203.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=NADH-ubiquinone dehydrogenase 24 KD subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073975(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073975 NCBI Caenorhabditis elegans F53F4.10 (F53F4.10) mRNA, complete cds. 11/11 None
SNAP00000012447 ENSEMBL cdna:SNAP chromosome:CEL140:V:13627332:13628141:-1 11/11 None
GENEFINDER00000012455 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13627332:13628141:-1 11/11 None
F53F4.10.1 ENSEMBL cdna:known chromosome:CEL140:V:13627216:13628159:-1 gene:F53F4.10 11/11 None
F53F4.10.2 ENSEMBL cdna:known chromosome:CEL140:V:13627332:13628160:-1 gene:F53F4.10 11/11 None
F53F4.10.3 ENSEMBL cdna:known chromosome:CEL140:V:13627332:13628161:-1 gene:F53F4.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13627331-13628141(-) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-ubiquinone dehydrogenase 24 KD subunit
Ensembl F53F4.10
Entrez Gene 179850 Entrez gene
SwissProt Q20719 EMBL-EBI
WormBase CE10972 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13017_AT NADH-ubiquinone oxidoreductase 24 kDa subunit, putative at
ATH1-121501:255442_AT NADH-ubiquinone oxidoreductase 24 kDa subunit, putative at
CANINE:1587401_AT similar to NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa cfa
CANINE_2:CFAAFFX.28547.1.S1_AT similar to NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa cfa
DROSOPHILA_2:1628014_AT dm
DROSGENOME1:152209_AT dm
CHICKEN:GGA.4778.1.S1_A_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa gga
CHICKEN:GGA.4778.2.S1_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa gga
HUGENEFL:M22538_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
HG-U133A:202941_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
HG-U133_PLUS_2:202941_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
HG-FOCUS:202941_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
HG-U133A_2:202941_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
U133_X3P:G10835024_3P_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
HG-U95AV2:34893_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
HG-U95AV2:31359_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
HG-U95C:58052_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
HU35KSUBB:RC_W90549_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa hs
MU11KSUBA:AA683778_S_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MG-U74AV2:94062_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOE430A:1428179_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOUSE430A_2:1428179_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOUSE430_2:1428179_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOE430A:1452692_A_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOUSE430A_2:1452692_A_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOUSE430_2:1452692_A_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOE430A:1438159_X_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOUSE430A_2:1438159_X_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MOUSE430_2:1438159_X_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 mm
MG-U74CV2:137370_AT NADH dehydrogenase (ubiquinone) flavoprotein 2, mRNA (cDNA clone MGC:32165 IMAGE:5003524) mm
RT-U34:M22756_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 rn
RG-U34A:M22756_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 rn
RAE230A:1371041_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 rn
RAT230_2:1371041_AT NADH dehydrogenase (ubiquinone) flavoprotein 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6120 mitochondrial electron transport, NADH to ubiquinone inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8137 NADH dehydrogenase (ubiquinone) activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01203 Hypothetical protein F53F4.10 [Caenorhabditis elegans] ref|NP_506376.1| F53F4.10 [Caenorhabditis elegans] sp|Q20719|NUHM_CAEEL NADH-ubiquinone oxidoreductase 24 kDa subunit, mitochondrial precursor 1.0E-134
blast CAE66274 Hypothetical protein CBG11518 [Caenorhabditis briggsae] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR002023 EMBL-EBI NADH dehydrogenase (ubiquinone), 24 kDa subunit 5.9E-102
Sequence
Target Sequence 
>C. ELEGANS:193550_S_AT
cttattccacttctcgatttggctcagcggcaacatggctggcttccaatttcagccatg
catgaggttgctaaaattttagaagtaccaagaatgcgtgcttatgaagttgccactttc
tacaccatgttcaacaggcaaccagtaggaaagtatttcctccaggtctgcgcaactacc
ccatgcatgcttcgaggagctgaaaccatcacggaaactattgagaagaaacttggcatt
catgccggagaaacaacaaaagatggactcttcacattggcggaagtagagtgccttgga
gcatgtgtcaatgctccaatgattcaaattaacgacgattatttcgaggacctgacacca
aaggatgtgaacgagattcttgacgatcttaaagcaggacgtaaaccagctgctggacca
agatccgggcgcctagccgctgagccattcggagagcttacttctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTATTCCACTTCTCGATTTGGCTC 665 221 214 Antisense
GATTTGGCTCAGCGGCAACATGGCT 561 425 229 Antisense
GCAACATGGCTGGCTTCCAATTTCA 449 319 243 Antisense
CAATTTCAGCCATGCATGAGGTTGC 174 181 260 Antisense
ATGAAGTTGCCACTTTCTACACCAT 197 45 317 Antisense
TACCCCATGCATGCTTCGAGGAGCT 42 647 390 Antisense
GAGGAGCTGAAACCATCACGGAAAC 139 405 407 Antisense
AAGATGGACTCTTCACATTGGCGGA 259 155 473 Antisense
GAGTGCCTTGGAGCATGTGTCAATG 238 401 502 Antisense
GATTATTTCGAGGACCTGACACCAA 40 429 550 Antisense
GAGCCATTCGGAGAGCTTACTTCTC 432 383 655 Antisense
Sequence Source Affymetrix Proprietary Database