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[193548_at] N-acetyl-glucosamine-6-phosphate deacetylase

Description F59B2.3 /REP_DB=WormBase Gene ID /WP=CE00231 /TR=SW:P34480 /GB=CAA77585.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=N-acetyl-glucosamine-6-phosphate deacetylase
Cluster 6grp_highest_10_10 > 49      6grp_high1_10_10 > 94      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 64      6grp_total > 74      4grp_total > 56      6grp_highest_sub > 04_05_06      6grp_high1_sub > In      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 286
1 2 3 4 range
A 500.6
(-3)
P 		611.6
(0.3)
P 		704.1
(3)
P 		511.4
(-2.7)
P 		204
B 560.9
(0.3)
P 		575.6
(0.6)
P 		681
(3)
P 		418.1
(-3)
P 		263
C 451.5
(-3)
P 		551.3
(0.5)
P 		624.2
(3)
P 		452.4
(-3)
P 		173
avr 504.3
(-1.7) 		579.5
(0.4) 		669.8
(3) 		460.6
(-3) 		209

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 608
1 2 3 4 5 6 range
A 526.1
(-3)
P 		547
(-2.7)
P 		779.2
(0.3)
P 		989.7
(3)
P 		912.7
(2)
P 		987.9
(3)
P 		464
B 497
(-3)
P 		582.4
(-2.1)
P 		828.5
(0.5)
P 		905.4
(1.3)
P 		1071.1
(3)
P 		971.5
(2)
P 		574
C 462.9
(-3)
P 		572.1
(-1.9)
P 		842.5
(0.9)
P 		1047.5
(3)
P 		796.4
(0.4)
P 		958
(2.1)
P 		585
avr 495.3
(-3) 		567.2
(-2.1) 		816.7
(1) 		980.9
(3) 		926.7
(2.3) 		972.5
(2.9) 		486
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 182.3
(2.9)
P 		132.8
(0.2)
P 		184.1
(3)
P 		74.9
(-3)
P 		109
B 178.9
(3)
P 		142.2
(0.5)
P 		157.8
(1.6)
P 		89.1
(-3)
P 		90
C 149.4
(0.1)
P 		158.5
(0.5)
P 		217.6
(3)
P 		76.3
(-3)
P 		141
avr 170.2
(2.1) 		144.5
(0.6) 		186.5
(3) 		80.1
(-3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 338
1 2 3 4 5 6 range
A 97
(-3)
P 		194.5
(0.3)
P 		169.7
(-0.6)
P 		251
(2.2)
P 		275.4
(3)
P 		240.5
(1.8)
P 		178
B 172.9
(-3)
P 		193.8
(-2.5)
P 		201.2
(-2.4)
P 		239.8
(-1.5)
P 		435.2
(3)
P 		362.3
(1.3)
P 		262
C 171.6
(-3)
P 		174.6
(-2.9)
P 		214.4
(-1.3)
P 		210.7
(-1.4)
P 		257.7
(0.5)
P 		320.7
(3)
P 		149
avr 147.2
(-3) 		187.6
(-1.6) 		195.1
(-1.4) 		233.8
(-0) 		322.8
(3) 		307.8
(2.5) 		176
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193548_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6033
Sequence Type Exemplar sequence
Representative Public ID F59B2.3 NCBI
Target Description F59B2.3 /REP_DB=WormBase Gene ID /WP=CE00231 /TR=SW:P34480 /GB=CAA77585.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=N-acetyl-glucosamine-6-phosphate deacetylase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066589(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066589 NCBI Caenorhabditis elegans F59B2.3 (F59B2.3) mRNA, complete cds. 11/11 None
GENEFINDER00000012590 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8994894:8999672:-1 11/11 None
SNAP00000012573 ENSEMBL cdna:SNAP chromosome:CEL140:III:8996894:9001693:-1 11/11 None
F59B2.3 ENSEMBL cdna:known chromosome:CEL140:III:8996796:8999672:-1 gene:F59B2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8996889-8999668(-) 100.0 100.0
Public Domain and Genome References
Gene Title N-acetyl-glucosamine-6-phosphate deacetylase
Ensembl F59B2.3
Entrez Gene 176272 Entrez gene
SwissProt P34480 EMBL-EBI
WormBase CE00231 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18442.1.S1_S_AT similar to CG17065-PA cfa
CANINE_2:CFAAFFX.29579.1.S1_S_AT similar to CG17065-PA cfa
DROSOPHILA_2:1638247_AT dm
DROSGENOME1:145416_AT dm
CHICKEN:GGA.10211.1.S1_AT similar to RIKEN cDNA 5730457F11 gga
CHICKEN:GGA.10211.2.S1_A_AT similar to RIKEN cDNA 5730457F11 gga
CHICKEN:GGAAFFX.5823.1.S1_S_AT similar to RIKEN cDNA 5730457F11 gga
HU35KSUBA:RC_AA243870_AT CGI-14 protein hs
HG-U133A:219082_AT CGI-14 protein hs
HG-U133_PLUS_2:219082_AT CGI-14 protein hs
HG-U133A_2:219082_AT CGI-14 protein hs
U133_X3P:G7705595_3P_AT CGI-14 protein hs
HG-U95C:48700_AT CGI-14 protein hs
HG-U95C:54270_AT CGI-14 protein hs
HG-U95E:91678_AT CGI-14 protein hs
HU35KSUBA:RC_AA207103_AT CGI-14 protein hs
HU35KSUBB:RC_N81018_AT CGI-14 protein hs
U133_X3P:HS.9204.1.S1_3P_X_AT CGI-14 protein hs
U133_X3P:HS.9204.1.S1_3P_AT CGI-14 protein hs
U133_X3P:HS.274361.2.A1_3P_AT CGI-14 protein hs
U133_X3P:227841_3P_AT CGI-14 protein hs
HG-U133A_2:222009_AT CGI-14 protein hs
HG-U133_PLUS_2:222009_AT CGI-14 protein hs
HG-U133A:222009_AT CGI-14 protein hs
HG-U133_PLUS_2:227841_AT CGI-14 protein hs
HG-U133B:227841_AT CGI-14 protein hs
HG-U95C:61363_AT CGI-14 protein hs
HG-U95C:63931_AT CGI-14 protein hs
MG-U74BV2:114644_AT RIKEN cDNA 5730457F11 gene mm
MOE430B:1434855_AT RIKEN cDNA 5730457F11 gene mm
MOUSE430_2:1434855_AT RIKEN cDNA 5730457F11 gene mm
MU19KSUBA:TC22426_AT RIKEN cDNA 5730457F11 gene, mRNA (cDNA clone MGC:47163 IMAGE:5101453) mm
MU19KSUBB:TC28416_AT RIKEN cDNA 5730457F11 gene, mRNA (cDNA clone MGC:47163 IMAGE:5101453) mm
RAE230A:1374936_AT similar to RIKEN cDNA 5730457F11 (predicted) rn
RAT230_2:1374936_AT similar to RIKEN cDNA 5730457F11 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6044 N-acetylglucosamine metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8448 N-acetylglucosamine-6-phosphate deacetylase activity inferred from electronic annotation QuickGO AmiGO
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA77585 Hypothetical protein F59B2.3 [Caenorhabditis elegans] ref|NP_498990.1| F59B2.3 [Caenorhabditis elegans] sp|P34480|NAGA_CAEEL Putative N-acetylglucosamine-6-phosphate deacetylase (GlcNAc 6-P deacetylase) 0.0
blast CAE62701 Hypothetical protein CBG06850 [Caenorhabditis briggsae] 0.0
blast CAA77582 Hypothetical protein F59B2.2 [Caenorhabditis elegans] ref|NP_498989.1| F59B2.2 [Caenorhabditis elegans] sp|P34479|YMJ2_CAEEL Hypothetical protein F59B2.2 in chromosome III 0.0
blast CAE62700 Hypothetical protein CBG06849 [Caenorhabditis briggsae] 0.0
blast CAA77584 Hypothetical protein F59B2.5 [Caenorhabditis elegans] ref|NP_498991.2| F59B2.5 [Caenorhabditis elegans] sp|P34481|YMJ5_CAEEL Hypothetical protein F59B2.5 in chromosome III 0.0
blast CAE62701 Hypothetical protein CBG06850 [Caenorhabditis briggsae] 1.0E-176
Protein Domains
Database ID Description E-Value
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.5E-5
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.6E-7
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 1.0E-126
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.5E-5
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.6E-7
Pfam IPR000717 EMBL-EBI Proteasome component region PCI 2.0E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000012590 10 481-503,544-566,586-603,610-632,642-661,681-703,733-755,776-793,797-819,840-862
Sequence
Target Sequence 
>C. ELEGANS:193548_AT
gaatatttcgtttccactggtaccaccgtatccgttggacattcatctgccaagcttgga
cccggagaaatggctgtactatctggtgcgaaaatgattacgcatttgttcaatgctatg
caatcgtaccatcatcgcgaccctggtctcatcggacttctgacttcatcaaaattgact
cctgatcatccattatattatggaatcatttccgatggaatacacactcatgactcagca
cttcgaattgcttaccatactaactctgctggtcttgttcttgtaaccgacgctattgct
gcacttgggatgtctgatggagttcacaaactcggaactcagaccattcatgtgaaaggt
cttgaagcgaaacttgatgggacaaatacgacggctggaagtgttgcgagtatgccatac
tgtattcggcatctcatgaaagctacagggtgtcccatcgaatttgctcttcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATATTTCGTTTCCACTGGTACCA 166 325 640 Antisense
TACCACCGTATCCGTTGGACATTCA 268 647 660 Antisense
GGACATTCATCTGCCAAGCTTGGAC 254 525 676 Antisense
TATGCAATCGTACCATCATCGCGAC 179 657 756 Antisense
CTCATCGGACTTCTGACTTCATCAA 137 229 787 Antisense
GAATACACACTCATGACTCAGCACT 259 327 857 Antisense
ATTGCTTACCATACTAACTCTGCTG 138 7 886 Antisense
GGTCTTGTTCTTGTAACCGACGCTA 192 503 910 Antisense
GCTATTGCTGCACTTGGGATGTCTG 132 305 931 Antisense
GCCATACTGTATTCGGCATCTCATG 400 281 1053 Antisense
TGTCCCATCGAATTTGCTCTTCAAA 468 557 1090 Antisense
Sequence Source Affymetrix Proprietary Database