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[193545_s_at] zinc metallopeptidase (M12B family)

Description F57B7.4 /REP_DB=WormBase Gene ID /WP=CE27763 /GEN=mig-17 /TR=Q20930 /GB=CAA98493.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metallopeptidase (M12B family)
Cluster 6grp_high1_10_10 > 52      6grp_total > 62      4grp_total > 50      6grp_cgc > 73      6grp_high1_sub > 03      6grp_cgc_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 245
1 2 3 4 range
A 84.3
(-2.2)
P 		296.6
(3)
P 		194.5
(0.5)
P 		52.7
(-3)
A 		244
B 95.6
(-1.9)
P 		282.3
(3)
P 		199.7
(0.9)
P 		51.7
(-3)
A 		231
C 89.1
(-2.2)
P 		241.9
(2.1)
P 		273.4
(3)
P 		59.1
(-3)
A 		214
avr 89.7
(-2) 		273.6
(3) 		222.5
(1.6) 		54.5
(-3) 		219

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 284
1 2 3 4 5 6 range
A 120.5
(-3)
M 		316.5
(1.7)
P 		369.2
(3)
P 		287.1
(1)
P 		178.7
(-1.6)
P 		157.3
(-2.1)
P 		249
B 94.2
(-3)
P 		261
(0.5)
P 		378.5
(3)
P 		296.1
(1.3)
P 		157.4
(-1.7)
P 		119.7
(-2.5)
P 		284
C 123.2
(-3)
P 		257.5
(2.4)
P 		271.3
(3)
P 		253.7
(2.3)
P 		133.5
(-2.6)
P 		130.3
(-2.7)
P 		148
avr 112.6
(-3) 		278.3
(1.4) 		339.7
(3) 		279
(1.4) 		156.5
(-1.8) 		135.8
(-2.4) 		227
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 30.7
(-0.6)
P 		64.4
(3)
P 		50.9
(1.6)
P 		7.7
(-3)
A 		57
B 30.5
(-1)
P 		69.7
(3)
P 		46.3
(0.6)
P 		11
(-3)
A 		59
C 29.5
(-1.6)
P 		69.5
(1.2)
P 		95.3
(3)
P 		10
(-3)
A 		85
avr 30.2
(-0.9) 		67.9
(3) 		64.2
(2.6) 		9.6
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 22.2
(-3)
M 		112.6
(3)
P 		80.4
(0.9)
P 		72.8
(0.4)
P 		53.9
(-0.9)
P 		38.3
(-1.9)
P 		90
B 32.8
(-3)
P 		86.9
(2.5)
P 		91.9
(3)
P 		78.4
(1.6)
P 		64
(0.2)
P 		44.6
(-1.8)
P 		59
C 45.7
(-2.6)
P 		78.6
(3)
P 		69.1
(1.4)
P 		51
(-1.7)
P 		43.2
(-3)
P 		43.6
(-2.9)
P 		35
avr 33.6
(-3) 		92.7
(3) 		80.5
(1.8) 		67.4
(0.4) 		53.7
(-1) 		42.2
(-2.1) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193545_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12586
Sequence Type Exemplar sequence
Representative Public ID F57B7.4 NCBI
Target Description F57B7.4 /REP_DB=WormBase Gene ID /WP=CE27763 /GEN=mig-17 /TR=Q20930 /GB=CAA98493.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metallopeptidase (M12B family)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073500(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073500 NCBI Caenorhabditis elegans abnormal cell MIGration family member (mig-17) (mig-17) mRNA, complete cds. 11/11 None
GENEFINDER00000018336 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11450115:11451808:1 11/11 None
F57B7.4 ENSEMBL cdna:known chromosome:CEL140:V:11449240:11451833:1 gene:F57B7.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000018331 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11449196-11451808(+) 100.0 100.0
Public Domain and Genome References
Gene Title zinc metallopeptidase (M12B family)
Gene Symbol mig-17
Ensembl F57B7.4
Entrez Gene 179575 Entrez gene
SwissProt Q20930 EMBL-EBI
WormBase CE31010 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4222 metalloendopeptidase activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98493 Hypothetical protein F57B7.4 [Caenorhabditis elegans] ref|NP_505901.2| abnormal cell MIGration family member (mig-17) [Caenorhabditis elegans] dbj|BAA96734.1| MIG-17 [Caenorhabditis elegans] 0.0
blast CAE75247 Hypothetical protein CBG23204 [Caenorhabditis briggsae] 0.0
blast CAE75247 Hypothetical protein CBG23204 [Caenorhabditis briggsae] 1.0E-173
blast XP_780466 PREDICTED: similar to a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 18, partial [Strongylocentrotus purpuratus] 9.0E-24
blast XP_780466 PREDICTED: similar to a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 18, partial [Strongylocentrotus purpuratus] 1.0E-23
Protein Domains
Database ID Description E-Value
Pfam IPR001590 EMBL-EBI Peptidase M12B, ADAM/reprolysin 1.7E-7
Pfam IPR001590 EMBL-EBI Peptidase M12B, ADAM/reprolysin 1.7E-7
Sequence
Target Sequence 
>C. ELEGANS:193545_S_AT
gctcacgaaattggtcactcacttggagcgcttcatgatggagcttatgaaactgctgaa
tgtgattcaaatgacaattatttaatggctgttgctgtatcaggaagtgcagatagacaa
agcttcttaaatagtcgacgaatgagtaattgtagcattaattcaattattgaaaatcta
aaagaaccaacagcaaattgtgtaaagaaatggaaaaccaaaaaaggaaaggatgtttca
caaaaagattttataaagaaaccaggagaacttgtaaaaattacaagacaatgccaagtt
gcatttggtcccacattcattccatgtttgcatatcggatacttccatgaacaatcaatt
tgtgaaaggatatggtgcagcgatggagagagtgatgaatgtcagacacttaattatttt
ccagcatttgatggaactgaatgcggatacaatatgtggtgccttgaaggtagctgtgtt
caaaataccaaaaaatggatggattgtaaagatattaattcaaagacatgttccaaatac
tcgacttcaaaattaaaacattattgtaaatcgaaagatttccgtgagatttgttgtaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCACGAAATTGGTCACTCACTTG 609 301 910 Antisense
CACTCACTTGGAGCGCTTCATGATG 585 197 925 Antisense
GGCTGTTGCTGTATCAGGAAGTGCA 302 541 996 Antisense
GCCAAGTTGCATTTGGTCCCACATT 591 277 1202 Antisense
ATTCATTCCATGTTTGCATATCGGA 300 11 1224 Antisense
ATATCGGATACTTCCATGAACAATC 312 21 1241 Antisense
GTCAGACACTTAATTATTTTCCAGC 167 467 1310 Antisense
GCGGATACAATATGTGGTGCCTTGA 453 293 1352 Antisense
GGTGCCTTGAAGGTAGCTGTGTTCA 84 503 1367 Antisense
GACATGTTCCAAATACTCGACTTCA 41 371 1434 Antisense
AGATTTCCGTGAGATTTGTTGTAGA 446 65 1485 Antisense
Sequence Source Affymetrix Proprietary Database