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[193537_s_at] Bumetanide-sensitive Na-K-C1 cotransporter

Description K02A2.3 /REP_DB=WormBase Gene ID /WP=CE02791 /TR=SW:Q09573 /GB=AAC46706.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Bumetanide-sensitive Na-K-C1 cotransporter
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 26      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 70      6grp_total > 25      4grp_total > 38      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 281
1 2 3 4 range
A 444.7
(3)
P 		259
(-1)
P 		163.4
(-3)
P 		279.4
(-0.5)
P 		281
B 344.2
(3)
P 		207.1
(-2)
P 		183
(-2.9)
P 		180
(-3)
P 		164
C 302.2
(3)
P 		223.4
(-1.4)
P 		193.9
(-3)
P 		231.2
(-0.9)
P 		108
avr 363.7
(3) 		229.8
(-1.4) 		180.1
(-3) 		230.2
(-1.4) 		184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 321
1 2 3 4 5 6 range
A 381
(3)
P 		314.2
(1.4)
P 		225
(-0.8)
P 		137.4
(-3)
P 		173.5
(-2.1)
P 		225.3
(-0.8)
P 		244
B 458.5
(3)
P 		428.6
(2.4)
P 		204.3
(-1.8)
P 		192.4
(-2)
P 		147.4
(-2.8)
P 		137.9
(-3)
P 		321
C 323.6
(1.8)
P 		367.6
(3)
P 		220
(-0.9)
P 		213.7
(-1.1)
P 		142.1
(-3)
P 		182.8
(-1.9)
P 		226
avr 387.7
(3) 		370.1
(2.5) 		216.4
(-1.4) 		181.2
(-2.3) 		154.3
(-3) 		182
(-2.3) 		233
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 161.9
(3)
P 		56.2
(-2.2)
P 		42.7
(-2.9)
P 		40.9
(-3)
P 		121
B 109.8
(3)
P 		51.2
(-1.9)
P 		42.4
(-2.7)
P 		38.4
(-3)
P 		71
C 100
(3)
P 		64.2
(-0.5)
P 		67.6
(-0.2)
P 		39
(-3)
P 		61
avr 123.9
(3) 		57.2
(-1.7) 		50.9
(-2.2) 		39.4
(-3) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 70.2
(-0.2)
P 		111.7
(3)
P 		49
(-1.9)
P 		34.9
(-3)
P 		52.3
(-1.6)
P 		54.9
(-1.4)
P 		77
B 159.5
(3)
P 		142.7
(2.1)
P 		49.6
(-3)
P 		51
(-2.9)
P 		59.9
(-2.4)
P 		51.4
(-2.9)
P 		110
C 119.9
(3)
P 		112.2
(2.4)
P 		56
(-2)
P 		43
(-3)
P 		46
(-2.8)
P 		61.2
(-1.6)
P 		77
avr 116.5
(2.6) 		122.2
(3) 		51.5
(-2.4) 		43
(-3) 		52.7
(-2.3) 		55.8
(-2) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193537_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5483
Sequence Type Exemplar sequence
Representative Public ID K02A2.3 NCBI
Target Description K02A2.3 /REP_DB=WormBase Gene ID /WP=CE02791 /TR=SW:Q09573 /GB=AAC46706.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Bumetanide-sensitive Na-K-C1 cotransporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063154(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063154 NCBI Caenorhabditis elegans K02A2.3 (K02A2.3) mRNA, complete cds. 11/11 None
SNAP00000023848 ENSEMBL cdna:SNAP chromosome:CEL140:II:7426014:7430261:-1 11/11 None
GENEFINDER00000023857 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7426014:7431320:-1 11/11 None
K02A2.3 ENSEMBL cdna:known chromosome:CEL140:II:7425940:7430394:-1 gene:K02A2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7426010-7430340(-) 97.55 100.0
Public Domain and Genome References
Gene Title Bumetanide-sensitive Na-K-C1 cotransporter
Ensembl K02A2.3
Entrez Gene 174212 Entrez gene
SwissProt Q09573 EMBL-EBI
WormBase CE02791 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46706 Hypothetical protein K02A2.3 [Caenorhabditis elegans] ref|NP_495555.1| K02A2.3 [Caenorhabditis elegans] sp|Q09573|YRD3_CAEEL Hypothetical protein K02A2.3 in chromosome II 0.0
blast CAE67537 Hypothetical protein CBG13060 [Caenorhabditis briggsae] 0.0
blast CAC80545 putative Na-K-Cl cotransporter [Meloidogyne incognita] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 3.8E-10
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.7E-13
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 3.8E-10
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 2.4E-24
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 3.8E-10
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 2.4E-24
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495555.1 11 70-92,102-124,137-159,164-181,202-224,228-250,377-399,414-436,457-479,514-533,540-562
K02A2.3 11 70-92,102-124,137-159,164-181,202-224,228-250,377-399,414-436,457-479,514-533,540-562
SNAP00000023848 10 67-89,94-116,152-174,195-217,221-243,371-390,405-427,507-526,532-554,566-588
GENEFINDER00000023857 11 97-119,129-151,164-186,191-208,229-251,255-277,405-424,439-461,541-560,566-588,600-622
Sequence
Target Sequence 
>C. ELEGANS:193537_S_AT
gatgatgcgtcatagcgattcagcaagaagttttagtccacagccaggtgcacatacttc
catcaacttggatgagacagaaacatcatttaccgaaagcctatttgatgatttctaccg
ttcgggaactcccaatgaagatctcgaaggtgcaatgaaactgaacattcacaaaatgaa
tacatccgttcgtctaaatcgtgttattcgagaaaattctccagactctcagttgatctt
attgaatctaccatctcctccaagaaaccgtcttgctttcaacaattcttatatgacata
tttggacgtgttgactgaagatttgccacgtgtacttttcattg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGATGCGTCATAGCGATTCAGCA 466 411 2694 Antisense
TAGTCCACAGCCAGGTGCACATACT 368 651 2727 Antisense
GTGCACATACTTCCATCAACTTGGA 355 481 2741 Antisense
ATTTGATGATTTCTACCGTTCGGGA 394 1 2796 Antisense
GGGAACTCCCAATGAAGATCTCGAA 346 493 2817 Antisense
TACATCCGTTCGTCTAAATCGTGTT 609 643 2874 Antisense
AAATTCTCCAGACTCTCAGTTGATC 639 115 2907 Antisense
ATCTTATTGAATCTACCATCTCCTC 17 31 2929 Antisense
CCATCTCCTCCAAGAAACCGTCTTG 565 271 2944 Antisense
AACCGTCTTGCTTTCAACAATTCTT 100 147 2959 Antisense
GATTTGCCACGTGTACTTTTCATTG 685 427 3013 Antisense
Sequence Source Affymetrix Proprietary Database