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[193531_at] guanine nucleotide-binding protein

Description F48C11.1 /REP_DB=WormBase Gene ID /WP=CE20829 /GEN=gpa-6 /TR=Q93743 /GB=CAB02553.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_high1_10_10 > 23      4grp_high_8_8 > 12      6grp_total > 02      4grp_total > 13      6grp_cgc > 52      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 73.1
(-1.5)
P 		51.1
(-3)
P 		63.3
(-2.2)
P 		140.6
(3)
P 		90
B 64.2
(0.9)
P 		63.9
(0.8)
P 		49.8
(-3)
P 		71.8
(3)
M 		22
C 114.8
(3)
P 		75.3
(-2.2)
P 		69.6
(-3)
A 		94.3
(0.3)
P 		45
avr 84
(0.4) 		63.4
(-2.6) 		60.9
(-3) 		102.2
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 119.8
(3)
P 		59
(-2.3)
P 		98.8
(1.2)
P 		89.2
(0.3)
P 		53.6
(-2.7)
P 		50.6
(-3)
A 		69
B 72.1
(-1.4)
P 		119
(3)
P 		56.7
(-2.8)
P 		54.9
(-3)
M 		67.9
(-1.8)
P 		65.3
(-2)
P 		64
C 72.6
(-1.4)
P 		125.2
(3)
P 		59.7
(-2.5)
P 		83.9
(-0.5)
P 		103.9
(1.2)
P 		53.7
(-3)
P 		72
avr 88.2
(1.3) 		101.1
(3) 		71.7
(-1) 		76
(-0.4) 		75.1
(-0.5) 		56.5
(-3) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 26.6
(3)
P 		11.1
(-3)
P 		16.5
(-0.9)
P 		20.6
(0.7)
P 		16
B 20.5
(3)
P 		15.8
(-0.1)
P 		11.5
(-3)
P 		15.3
(-0.5)
M 		9
C 38
(3)
P 		21.6
(-1.5)
P 		24.3
(-0.7)
A 		15.9
(-3)
P 		22
avr 28.4
(3) 		16.2
(-3) 		17.4
(-2.4) 		17.3
(-2.5) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 22.1
(2.7)
P 		21
(2.1)
P 		21.5
(2.4)
P 		22.6
(3)
P 		16.2
(-0.7)
P 		12.3
(-3)
A 		10
B 25.1
(-0.4)
P 		39.6
(3)
P 		13.8
(-3)
P 		14.5
(-2.8)
M 		27.6
(0.2)
P 		24.4
(-0.5)
P 		26
C 26.9
(0.1)
P 		38.2
(3)
P 		15.2
(-3)
P 		16.9
(-2.6)
P 		33.6
(1.8)
P 		18
(-2.3)
P 		23
avr 24.7
(-0.1) 		32.9
(3) 		16.8
(-3) 		18
(-2.6) 		25.8
(0.4) 		18.2
(-2.5) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193531_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17697
Sequence Type Exemplar sequence
Representative Public ID F48C11.1 NCBI
Target Description F48C11.1 /REP_DB=WormBase Gene ID /WP=CE20829 /GEN=gpa-6 /TR=Q93743 /GB=CAB02553.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077788(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077788 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-6) (gpa-6) mRNA, complete cds. 11/11 None
GENEFINDER00000030515 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13191166:13193875:1 11/11 None
SNAP00000030514 ENSEMBL cdna:SNAP chromosome:CEL140:X:13191399:13193875:1 11/11 None
F48C11.1 ENSEMBL cdna:known chromosome:CEL140:X:13191166:13193875:1 gene:F48C11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13191165-13193875(+) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-6
Ensembl F48C11.1
Entrez Gene 181442 Entrez gene
SwissProt Q93743 EMBL-EBI
WormBase CE20829 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02553 Hypothetical protein F48C11.1 [Caenorhabditis elegans] ref|NP_510189.1| G Protein, Alpha subunit family member (gpa-6) [Caenorhabditis elegans] gb|AAG32082.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q93743|GPA6_CAEEL Guanine nucleotide-binding protein alpha-6 subunit 0.0
blast AAW02905 gpa-6 [Caenorhabditis briggsae] sp|Q4VT31|GPA6_CAEBR Guanine nucleotide-binding protein alpha-6 subunit 0.0
blast CAE61396 Hypothetical protein CBG05252 [Caenorhabditis briggsae] 0.0
blast CAB02553 Hypothetical protein F48C11.1 [Caenorhabditis elegans] ref|NP_510189.1| G Protein, Alpha subunit family member (gpa-6) [Caenorhabditis elegans] gb|AAG32082.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q93743|GPA6_CAEEL Guanine nucleotide-binding protein alpha-6 subunit 1.0E-176
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.4E-125
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.6E-97
Sequence
Target Sequence 
>C. ELEGANS:193531_AT
gataactacgtcccaagtattcaagatattgtacactgccgtatttcaacaacggggatt
aacgaacttgcattcaattacaagaaaatggatttcaaaatggtagacgttggaggacaa
agaagcgaacgtcggaaatggatacactgttttgacaacgtcgacatgattctctttatt
gtttcaatgagcgattatgatcaattagatccggaagacaacaaatacaatcgcatgaaa
caaagctatgaaatattcaaaacaattgttcattcggatttatttcgccatgcatcaatt
gtcctatttctcaacaaatacgatgtttttgttgaaaaactgaaaacgtcaccgcttcga
agaagtttcaaaaactatgaaggagataactccgaagagagcgctcgtgagttcattaaa
aagctttttcgtcgatgtatcacggatcgccacaagttttttgtcttcgaaaccacagcg
acagatacggggaatattgatctggtatttggctcagcagttgcacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATAACTACGTCCCAAGTATTCAAG 608 421 538 Antisense
GATATTGTACACTGCCGTATTTCAA 305 427 562 Antisense
GACAACGTCGACATGATTCTCTTTA 362 365 691 Antisense
GTTCATTCGGATTTATTTCGCCATG 484 443 805 Antisense
TTTCGCCATGCATCAATTGTCCTAT 157 669 820 Antisense
AACTGAAAACGTCACCGCTTCGAAG 210 1 875 Antisense
GAAGAGAGCGCTCGTGAGTTCATTA 333 339 931 Antisense
TCGTCGATGTATCACGGATCGCCAC 532 605 966 Antisense
GGATCGCCACAAGTTTTTTGTCTTC 90 509 981 Antisense
GAAACCACAGCGACAGATACGGGGA 277 359 1006 Antisense
GGTATTTGGCTCAGCAGTTGCACAT 637 505 1041 Antisense
Sequence Source Affymetrix Proprietary Database