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[193527_at]

Description F27D9.1 /REP_DB=WormBase Gene ID /WP=CE24927 /GEN=unc-18 /TR=SW:P34815 /GB=AAA93389.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Acetylcholine regulator unc-18
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 31      6grp_total > 24      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1149
1 2 3 4 range
A 1517.2
(3)
P 		724.8
(-1.5)
P 		716.7
(-1.5)
P 		456
(-3)
P 		1061
B 1570.3
(3)
P 		811.3
(-1.3)
P 		675.3
(-2.1)
P 		516.9
(-3)
P 		1053
C 1512.6
(3)
P 		858.7
(-0.6)
P 		742
(-1.2)
P 		421.2
(-3)
P 		1091
avr 1533.4
(3) 		798.3
(-1.1) 		711.3
(-1.6) 		464.7
(-3) 		1069

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 898
1 2 3 4 5 6 range
A 1009.5
(-0.1)
P 		1408.8
(3)
P 		676.5
(-2.6)
P 		852.5
(-1.3)
P 		625.7
(-3)
P 		693.8
(-2.5)
P 		783
B 963.3
(1.1)
P 		1167.6
(3)
P 		974.7
(1.2)
P 		621.8
(-2.1)
P 		580.7
(-2.4)
P 		519.6
(-3)
P 		648
C 1417.8
(3)
P 		1084.8
(0.8)
P 		1232.8
(1.8)
P 		873.4
(-0.7)
P 		710
(-1.8)
P 		529.6
(-3)
P 		888
avr 1130.2
(2.2) 		1220.4
(3) 		961.3
(0.6) 		782.6
(-1.1) 		638.8
(-2.5) 		581
(-3) 		639
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 486
1 2 3 4 range
A 552.4
(3)
P 		157.3
(-1.9)
P 		187.3
(-1.5)
P 		66.7
(-3)
P 		486
B 500.8
(3)
P 		200.4
(-1.6)
P 		156.5
(-2.3)
P 		110.1
(-3)
P 		391
C 500.7
(3)
P 		246.9
(-0.5)
P 		258.6
(-0.4)
P 		71
(-3)
P 		430
avr 518
(3) 		201.5
(-1.4) 		200.8
(-1.4) 		82.6
(-3) 		435

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 378
1 2 3 4 5 6 range
A 186.1
(-2.3)
P 		501.1
(3)
P 		147.3
(-3)
P 		216.2
(-1.8)
P 		188.8
(-2.3)
P 		168.9
(-2.6)
P 		354
B 335.2
(1.6)
P 		388.7
(3)
P 		236.7
(-1.1)
P 		164.7
(-3)
P 		235.9
(-1.1)
P 		193.7
(-2.2)
P 		224
C 525.5
(3)
P 		331
(-0.3)
P 		313.8
(-0.6)
P 		175.6
(-3)
P 		229.8
(-2.1)
P 		177.3
(-3)
P 		350
avr 348.9
(1.5) 		406.9
(3) 		232.6
(-1.6) 		185.5
(-2.9) 		218.2
(-2) 		180
(-3) 		227
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193527_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16233
Sequence Type Exemplar sequence
Representative Public ID F27D9.1 NCBI
Target Description F27D9.1 /REP_DB=WormBase Gene ID /WP=CE24927 /GEN=unc-18 /TR=SW:P34815 /GB=AAA93389.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Acetylcholine regulator unc-18
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029437(11), NM_001029435(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029437 NCBI Caenorhabditis elegans UNCoordinated family member (unc-18) (unc-18) mRNA, complete cds. 11/11 None
NM_001029435 NCBI Caenorhabditis elegans UNCoordinated family member (unc-18) (unc-18) mRNA, complete cds. 11/11 None
SNAP00000002522 ENSEMBL cdna:SNAP chromosome:CEL140:X:7683896:7685175:1 11/11 None
GENEFINDER00000002527 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7683896:7685175:1 11/11 None
F27D9.1c ENSEMBL cdna:known chromosome:CEL140:X:7682357:7686329:1 gene:F27D9.1 11/11 A 
F27D9.1a.2 ENSEMBL cdna:known chromosome:CEL140:X:7682833:7685175:1 gene:F27D9.1 11/11 None
F27D9.1a.1 ENSEMBL cdna:known chromosome:CEL140:X:7682835:7685175:1 gene:F27D9.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181030 3 2 NM_001029435 193527_at (11)
NM_001029437 174516_at (11), 193527_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7682869-7685174(+) 100.0 100.0
Public Domain and Genome References
Ensembl F27D9.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAQ81273 Uncoordinated protein 18, isoform c [Caenorhabditis elegans] ref|NP_001024608.1| UNCoordinated family member (unc-18) [Caenorhabditis elegans] gb|AAB21818.1| putative acetylcholine regulator [Caenorhabditis elegans] 0.0
blast AAA93389 Uncoordinated protein 18, isoform a [Caenorhabditis elegans] ref|NP_001024606.1| UNCoordinated family member (unc-18) [Caenorhabditis elegans] gb|AAB28570.1| UNC-18 [Caenorhabditis elegans] sp|P34815|UNC18_CAEEL Putative acetylcholine regulator unc-18 (Uncoordinated protein 18) 0.0
blast CAE70259 Hypothetical protein CBG16760 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001619 EMBL-EBI Sec1-like protein 1.0E-126
Pfam IPR001619 EMBL-EBI Sec1-like protein 1.0E-126
Pfam IPR001619 EMBL-EBI Sec1-like protein 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193527_AT
tggtgccacttttgattgacccagccgtgcggtgtgaagaccgcctcagattgattctgt
tgtacattctttccaagaatggaattactgatgaaaacttgaataaacttttacaacacg
ctaatatctccatggctgataaggaaacaatcaccaacgctgcttacttgggattgaata
ttgttaccgataccggccgcaagaagacgtggaccccaaccaaaaaggaaagaccacacg
agcaagtttaccaatcttcccgctgggttccagttatcaaggatattattgaagatgcta
ttgacgagcgtttggacacaaaacatttcccattccttgctggacgacaagttaaccaag
gatacagagcccctgcatctgctagatatggtcagtggcacaaggaacgaggacaacaat
ccaactatcgcagtggaccacgactgattatctacattattggaggtgtcaccttctccg
aaatgcgagcttgttatgaagtgaccgctgcccgaaaaccgtgggaagtcgtcattgggt
ccgacagaatcatcactcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGTGCCACTTTTGATTGACCCAGC 327 553 1178 Antisense
GACCCAGCCGTGCGGTGTGAAGACC 457 381 1195 Antisense
GTGAAGACCGCCTCAGATTGATTCT 472 481 1211 Antisense
AACACGCTAATATCTCCATGGCTGA 49 137 1292 Antisense
ACGAGCAAGTTTACCAATCTTCCCG 493 93 1415 Antisense
CAAAACATTTCCCATTCCTTGCTGG 464 191 1496 Antisense
AGCCCCTGCATCTGCTAGATATGGT 321 83 1545 Antisense
GTGGACCACGACTGATTATCTACAT 336 491 1610 Antisense
TGTCACCTTCTCCGAAATGCGAGCT 114 559 1644 Antisense
TTATGAAGTGACCGCTGCCCGAAAA 113 681 1671 Antisense
TTGGGTCCGACAGAATCATCACTCC 26 709 1712 Antisense
Sequence Source Affymetrix Proprietary Database