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[193520_at]

Description W06B3.2 /REP_DB=WormBase Gene ID /WP=CE27220 /TR=Q9XTI6 /GB=CAB03981.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase (CDC2-CDC28 subfamily)
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 19      4grp_highest_8_8 > 28      4grp_high_8_8 > 0      6grp_highest_nonorm > 70      6grp_total > 29      4grp_total > 0      6grp_cgc > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 230
(-3)
P 		268.4
(3)
P 		237.6
(-1.8)
P 		257.5
(1.3)
P 		38
B 254.6
(3)
P 		185.6
(-3)
P 		222.5
(0.2)
P 		234.7
(1.3)
P 		69
C 223.2
(-1.4)
P 		190.1
(-3)
P 		311.6
(3)
P 		240.5
(-0.5)
P 		122
avr 235.9
(-0) 		214.7
(-3) 		257.2
(3) 		244.2
(1.2) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 441
1 2 3 4 5 6 range
A 238.5
(-0.2)
P 		347.1
(3)
P 		140.8
(-3)
P 		189.8
(-1.6)
P 		283.3
(1.1)
P 		248.8
(0.1)
P 		206
B 341.6
(1.5)
P 		285
(-0.8)
P 		231.1
(-3)
P 		254.9
(-2)
P 		378.4
(3)
P 		236.8
(-2.8)
P 		147
C 581.3
(3)
P 		329.1
(-0.6)
P 		164.2
(-3)
P 		224.1
(-2.1)
P 		261.8
(-1.6)
P 		230.7
(-2)
P 		417
avr 387.1
(3) 		320.4
(1.1) 		178.7
(-3) 		222.9
(-1.7) 		307.8
(0.7) 		238.8
(-1.3) 		208
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 83.7
(3)
P 		58.3
(-0.3)
P 		62.1
(0.2)
P 		37.7
(-3)
P 		46
B 81.2
(3)
P 		45.8
(-3)
P 		51.5
(-2)
P 		50
(-2.3)
P 		35
C 73.9
(-0.1)
P 		54.6
(-1.8)
P 		108.6
(3)
P 		40.6
(-3)
P 		68
avr 79.6
(3) 		52.9
(-1.4) 		74.1
(2.1) 		42.8
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 185
1 2 3 4 5 6 range
A 44
(-2.1)
P 		123.4
(3)
P 		30.7
(-3)
P 		48.1
(-1.9)
P 		85.5
(0.5)
P 		60.6
(-1.1)
P 		93
B 118.9
(0.9)
P 		94.9
(-0.6)
P 		56.1
(-3)
P 		67.5
(-2.3)
P 		153.7
(3)
P 		88.3
(-1)
P 		98
C 215.5
(3)
P 		100.4
(-1)
P 		41.8
(-3)
P 		45.1
(-2.9)
P 		84.7
(-1.5)
P 		77.2
(-1.8)
P 		174
avr 126.1
(3) 		106.2
(1.6) 		42.9
(-3) 		53.6
(-2.2) 		108
(1.7) 		75.4
(-0.7) 		83
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193520_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16868
Sequence Type Exemplar sequence
Representative Public ID W06B3.2 NCBI
Target Description W06B3.2 /REP_DB=WormBase Gene ID /WP=CE27220 /TR=Q9XTI6 /GB=CAB03981.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase (CDC2-CDC28 subfamily)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171783, NM_171782
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
W06B3.2b ENSEMBL cdna:known chromosome:CEL140:X:11997458:12001266:-1 gene:W06B3.2 None
W06B3.2a ENSEMBL cdna:known chromosome:CEL140:X:11997943:12005013:-1 gene:W06B3.2 None
GENEFINDER00000035064 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11998421:12001266:-1 None
SNAP00000035061 ENSEMBL cdna:SNAP chromosome:CEL140:X:11999856:12000548:-1 None
NM_171782 NCBI Caenorhabditis elegans SMAll family member (sma-5) (sma-5) mRNA, complete cds. None
NM_171783 NCBI Caenorhabditis elegans SMAll family member (sma-5) (sma-5) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000035060 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11998331-12000548(-) 100.0 100.0
Public Domain and Genome References
Ensembl W06B3.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD44110 Hypothetical protein W06B3.2b [Caenorhabditis elegans] emb|CAD44158.1| Hypothetical protein W06B3.2b [Caenorhabditis elegans] ref|NP_741908.1| SMAll family member (sma-5) [Caenorhabditis elegans] 0.0
blast CAB03981 Hypothetical protein W06B3.2a [Caenorhabditis elegans] emb|CAA18361.3| Hypothetical protein W06B3.2a [Caenorhabditis elegans] ref|NP_741909.1| SMAll family member (sma-5) [Caenorhabditis elegans] 0.0
blast CAJ21556 Hypothetical protein W06B3.2d [Caenorhabditis elegans] emb|CAJ21562.1| Hypothetical protein W06B3.2d [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-70
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-70
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 4.0E-66
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 2.0E-66
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 9.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.8E-74
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-26
Sequence
Target Sequence 
>C. ELEGANS:193520_AT
tccttcccgaacattcaacagcagttgacatgtgggcggttggatgtatctttggagaaa
tggtcattagaaatgaaattctgccgggaagaagtgttcaaggacagatcaaaatgcttt
tgactatgcttggacaacctcctcaagaagttattaatgaggttcgatgtgatcgcacaa
ggaagttgattcaagacttcggaaggaaagcggacgcagaatgggatgatattatgttct
gcaaagctcgaggagatgatcaaattgttcggggaaactgtgacactattgattttgtga
agcaactgttccaatatgatgcccagaagaggataaacattcaagatgctctcctacatc
catacatccagagagttattccagcagaagcaccacagaaaaaatgtccattcagagtga
agaaagatatgatgcaagttgaagatctcaatcatcaagaattgatcagcatgatgaagc
aagatgtccgatcagcggaaaacccaatcacatacagtgaactgcactctggtggtaaga
actttgcttttacagtctttctgttttgctttttgctaacaactcacgtgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTTCCCGAACATTCAACAGCAGT 696 591 662 Antisense
ATGTGGGCGGTTGGATGTATCTTTG 539 49 691 Antisense
TGACTATGCTTGGACAACCTCCTCA 313 571 782 Antisense
GCAACTGTTCCAATATGATGCCCAG 689 319 963 Antisense
ATTCAAGATGCTCTCCTACATCCAT 406 11 1000 Antisense
TCCTACATCCATACATCCAGAGAGT 690 591 1013 Antisense
GAGTTATTCCAGCAGAAGCACCACA 260 399 1034 Antisense
ATCAGCGGAAAACCCAATCACATAC 135 27 1152 Antisense
AGTGAACTGCACTCTGGTGGTAAGA 532 57 1177 Antisense
GAACTTTGCTTTTACAGTCTTTCTG 579 349 1200 Antisense
GCTTTTTGCTAACAACTCACGTGTG 508 303 1229 Antisense
Sequence Source Affymetrix Proprietary Database