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[193514_at] mannosyl-oligosaccharide alpha-1,2-mannosidase

Description F10C2.5 /REP_DB=WormBase Gene ID /WP=CE09309 /TR=P90830 /GB=CAB04078.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=mannosyl-oligosaccharide alpha-1,2-mannosidase
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 34      4grp_high_8_8 > 49      6grp_highest_nonorm > 63      6grp_total > 60      4grp_total > 62      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 390
1 2 3 4 range
A 526.5
(3)
P 		430.7
(1.2)
P 		376.3
(0.2)
P 		210.1
(-3)
P 		316
B 599.7
(3)
P 		378.6
(-0.9)
P 		419.1
(-0.1)
P 		255.5
(-3)
P 		344
C 541.4
(3)
P 		324.3
(-2)
P 		520.4
(2.5)
P 		278.3
(-3)
P 		263
avr 555.9
(3) 		377.9
(-0.5) 		438.6
(0.7) 		248
(-3) 		308

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 459
1 2 3 4 5 6 range
A 453.7
(-3)
P 		545.9
(-1.4)
P 		810.1
(3)
P 		780
(2.5)
P 		464.1
(-2.8)
P 		476
(-2.6)
P 		356
B 479.4
(-3)
P 		564.5
(-0.6)
P 		687.9
(3)
P 		611
(0.8)
P 		507.4
(-2.2)
P 		571.3
(-0.4)
P 		209
C 408
(-2.2)
P 		589.8
(0.3)
P 		779.5
(3)
P 		743.7
(2.5)
P 		350.7
(-3)
P 		609.5
(0.6)
P 		429
avr 447
(-2.9) 		566.7
(-0.6) 		759.2
(3) 		711.6
(2.1) 		440.7
(-3) 		552.3
(-0.9) 		319
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 191.7
(3)
P 		93.5
(-0.7)
P 		98.4
(-0.5)
P 		30.8
(-3)
P 		161
B 191.2
(3)
P 		93.5
(-1.3)
P 		97.1
(-1.1)
P 		54.4
(-3)
P 		137
C 179.2
(2.9)
P 		93.2
(-0.9)
P 		181.4
(3)
P 		46.9
(-3)
P 		135
avr 187.4
(3) 		93.4
(-0.9) 		125.6
(0.4) 		44
(-3) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 83.6
(-3)
P 		194.2
(2.8)
P 		176.4
(1.9)
P 		197.8
(3)
P 		140
(-0)
P 		115.9
(-1.3)
P 		114
B 166.8
(-2.4)
P 		187.9
(0.1)
P 		167
(-2.4)
P 		161.8
(-3)
P 		206.1
(2.2)
P 		213
(3)
P 		51
C 151.2
(-0.5)
P 		180
(1.4)
P 		198.4
(2.6)
P 		149.6
(-0.6)
P 		113.5
(-3)
P 		204.1
(3)
P 		91
avr 133.9
(-3) 		187.4
(3) 		180.6
(2.2) 		169.7
(1) 		153.2
(-0.8) 		177.7
(1.9) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193514_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13506
Sequence Type Exemplar sequence
Representative Public ID F10C2.5 NCBI
Target Description F10C2.5 /REP_DB=WormBase Gene ID /WP=CE09309 /TR=P90830 /GB=CAB04078.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=mannosyl-oligosaccharide alpha-1,2-mannosidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073617(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073617 NCBI Caenorhabditis elegans F10C2.5 (F10C2.5) mRNA, complete cds. 11/11 None
SNAP00000025560 ENSEMBL cdna:SNAP chromosome:CEL140:V:12048020:12051018:1 11/11 None
GENEFINDER00000025571 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12048020:12051018:1 11/11 None
F10C2.5 ENSEMBL cdna:known chromosome:CEL140:V:12048020:12051205:1 gene:F10C2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12048019-12051018(+) 100.0 100.0
Public Domain and Genome References
Gene Title mannosyl-oligosaccharide alpha-1,2-mannosidase
Ensembl F10C2.5
Entrez Gene 179653 Entrez gene
SwissProt P90830 EMBL-EBI
WormBase CE09309 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249092_AT glycoside hydrolase family 47 protein at
CANINE:1599112_AT similar to ER degradation-enhancing-mannosidase-like protein 2 cfa
CANINE:1594063_AT similar to ER degradation-enhancing-mannosidase-like protein 2 cfa
CANINE:1583939_S_AT similar to ER degradation-enhancing-mannosidase-like protein 2 cfa
CANINE_2:CFA.14431.1.A1_AT similar to ER degradation-enhancing-mannosidase-like protein 2 cfa
CANINE_2:CFA.2977.1.A1_S_AT similar to ER degradation-enhancing-mannosidase-like protein 2 cfa
CANINE_2:CFA.2977.2.A1_AT similar to ER degradation-enhancing-mannosidase-like protein 2 cfa
DROSOPHILA_2:1637602_AT dm
DROSOPHILA_2:1632200_S_AT dm
DROSGENOME1:152601_AT dm
HG-U133_PLUS_2:218282_AT chromosome 20 open reading frame 31 hs
HG-U133A:218282_AT chromosome 20 open reading frame 31 hs
HG-U133A_2:218282_AT chromosome 20 open reading frame 31 hs
HU35KSUBC:RC_AA251769_AT chromosome 20 open reading frame 31 hs
U133_X3P:G8922666_3P_AT chromosome 20 open reading frame 31 hs
HG-U95B:55652_AT chromosome 20 open reading frame 31 hs
MU11KSUBA:AA389917_S_AT RIKEN cDNA 9530090G24 gene mm
MG-U74AV2:160789_AT RIKEN cDNA 9530090G24 gene mm
MG-U74BV2:164887_I_AT RIKEN cDNA 9530090G24 gene mm
MOE430A:1423695_AT RIKEN cDNA 9530090G24 gene mm
MOUSE430A_2:1423695_AT RIKEN cDNA 9530090G24 gene mm
MOUSE430_2:1423695_AT RIKEN cDNA 9530090G24 gene mm
MOE430B:1444335_AT RIKEN cDNA 9530090G24 gene mm
MOUSE430_2:1444335_AT RIKEN cDNA 9530090G24 gene mm
MU19KSUBB:TC31281_AT RIKEN cDNA 9530090G24 gene (9530090G24Rik), mRNA mm
RG-U34B:RC_AI030929_AT similar to putative alpha-mannosidase rn
RAE230A:1376315_AT similar to putative alpha-mannosidase rn
RAT230_2:1376315_AT similar to putative alpha-mannosidase rn
RG-U34C:RC_AI145389_AT Similar to putative alpha-mannosidase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6487 protein amino acid N-linked glycosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04078 Hypothetical protein F10C2.5 [Caenorhabditis elegans] ref|NP_506018.1| F10C2.5 [Caenorhabditis elegans] 0.0
blast CAE75374 Hypothetical protein CBG23361 [Caenorhabditis briggsae] 0.0
blast EAL31574 GA17701-PA [Drosophila pseudoobscura] 1.0E-165
Protein Domains
Database ID Description E-Value
Pfam IPR001382 EMBL-EBI Glycoside hydrolase, family 47 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193514_AT
ggatcaactctacaaagcatacccaaatcatacggcagagttggaaaatttgaataaaga
agcaattattgaatttgaagaacttcaacatttggaggatattcgagaacttgttagaga
aatcccgatacctgaagttttttgtgagaaatgctgtgtcccacttcatgaaattgtcga
ttcggttgtacttcgagtgttaatggccgatgtttacgaatatcatttttatccaacgag
aaaaacaaaatttcttcatggtcctgtttgttggttagaggaaacaccacggattgagga
tgattacaaaaatgagccaccaagtgtgaacacagaaaaatggaatagtcctctcttcac
aagtgatcaggcttctctcaatgattttatcggagacgatcttgaaactgttcgaaagtt
tggattcaaaactcttctggctcctggttacaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATCAACTCTACAAAGCATACCCA 492 509 1833 Antisense
AGCATACCCAAATCATACGGCAGAG 512 75 1848 Antisense
GAGAAATCCCGATACCTGAAGTTTT 59 369 1949 Antisense
AGAAATGCTGTGTCCCACTTCATGA 258 73 1979 Antisense
GTCGATTCGGTTGTACTTCGAGTGT 55 471 2008 Antisense
GAGTGTTAATGGCCGATGTTTACGA 303 401 2027 Antisense
AAATTTCTTCATGGTCCTGTTTGTT 86 117 2080 Antisense
AATAGTCCTCTCTTCACAAGTGATC 237 173 2176 Antisense
ACAAGTGATCAGGCTTCTCTCAATG 108 115 2191 Antisense
GGATTCAAAACTCTTCTGGCTCCTG 45 509 2254 Antisense
ACTCTTCTGGCTCCTGGTTACAAAA 273 99 2263 Antisense
Sequence Source Affymetrix Proprietary Database