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[193502_s_at]

Description F13D12.4 /REP_DB=WormBase Gene ID /WP=CE02183 /TR=SW:P52713 /GB=CAA88946.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=methylmalonate-semialdehyde dehydrogenase
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 11      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 05      6grp_total > 23      4grp_total > 03      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26263
1 2 3 4 range
A 8544.8
(-2.2)
P 		4938.9
(-3)
P 		5175.6
(-2.9)
P 		31202.3
(3)
P 		26263
B 7672.6
(-2.3)
P 		5027.7
(-3)
P 		5220.5
(-2.9)
P 		27387.1
(3)
P 		22359
C 7886.4
(-2.2)
P 		5049
(-3)
P 		5198.9
(-3)
P 		27696.9
(3)
P 		22648
avr 8034.6
(-2.2) 		5005.2
(-3) 		5198.3
(-3) 		28762.1
(3) 		23757

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5622
1 2 3 4 5 6 range
A 8040.1
(3)
P 		6689.6
(0.9)
P 		6368
(0.4)
P 		5702.3
(-0.6)
P 		4467.7
(-2.5)
P 		4148.5
(-3)
P 		3892
B 7541.4
(3)
P 		6319.6
(1.3)
P 		6464.8
(1.5)
P 		5624.2
(0.4)
P 		4151.8
(-1.7)
P 		3176.3
(-3)
P 		4365
C 7529.3
(2.5)
P 		5971.1
(0.8)
P 		8026
(3)
P 		5264.2
(0)
P 		5675.7
(0.5)
P 		2417.8
(-3)
P 		5608
avr 7703.6
(3) 		6326.8
(1.1) 		6952.9
(2) 		5530.2
(0.1) 		4765.1
(-1) 		3247.5
(-3) 		4456
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4764
1 2 3 4 range
A 3111.2
(0.5)
P 		1072.1
(-3)
P 		1353
(-2.5)
P 		4567.4
(3)
P 		3495
B 2446.7
(-1.4)
P 		1241.7
(-3)
P 		1209.7
(-3)
P 		5836.2
(3)
P 		4627
C 2610.6
(-0.8)
P 		1451.6
(-3)
P 		1812
(-2.3)
P 		4670.1
(3)
P 		3219
avr 2722.8
(-0.7) 		1255.1
(-3) 		1458.2
(-2.7) 		5024.6
(3) 		3770

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1981
1 2 3 4 5 6 range
A 1482.1
(-0.9)
P 		2379.1
(3)
P 		1386.8
(-1.3)
P 		1446.2
(-1.1)
P 		1348.2
(-1.5)
P 		1010
(-3)
P 		1369
B 2624
(3)
P 		2103.5
(0.8)
P 		1569.8
(-1.4)
P 		1489.7
(-1.7)
P 		1686.8
(-0.9)
P 		1184.4
(-3)
P 		1440
C 2790.8
(3)
P 		1822
(0.1)
P 		2042.8
(0.7)
P 		1058.6
(-2.2)
P 		1836.6
(0.1)
P 		809.4
(-3)
P 		1981
avr 2299
(3) 		2101.5
(2.1) 		1666.5
(0.1) 		1331.5
(-1.5) 		1623.9
(-0.1) 		1001.3
(-3) 		1298
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193502_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2746
Sequence Type Exemplar sequence
Representative Public ID F13D12.4 NCBI
Target Description F13D12.4 /REP_DB=WormBase Gene ID /WP=CE02183 /TR=SW:P52713 /GB=CAA88946.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=methylmalonate-semialdehyde dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026907(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026907 NCBI Caenorhabditis elegans ALdehyde deHydrogenase family member (alh-8) (alh-8) mRNA, complete cds. 11/11 None
SNAP00000015030 ENSEMBL cdna:SNAP chromosome:CEL140:II:11728984:11730938:1 11/11 None
GENEFINDER00000015051 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11728984:11730938:1 11/11 None
F13D12.4a.4 ENSEMBL cdna:known chromosome:CEL140:II:11728854:11731126:1 gene:F13D12.4 11/11 None
F13D12.4a.2 ENSEMBL cdna:known chromosome:CEL140:II:11728921:11731127:1 gene:F13D12.4 11/11 None
F13D12.4a.3 ENSEMBL cdna:known chromosome:CEL140:II:11728921:11731179:1 gene:F13D12.4 11/11 None
F13D12.4a.1 ENSEMBL cdna:known chromosome:CEL140:II:11728960:11731127:1 gene:F13D12.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026908 7/11 Cross Hyb Matching Probes None
F13D12.4b 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174800 2 1 NM_001026907 193502_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11728980-11730935(+) 100.0 100.0
Public Domain and Genome References
Ensembl F13D12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88946 Hypothetical protein F13D12.4a [Caenorhabditis elegans] ref|NP_001022078.1| ALdehyde deHydrogenase family member (alh-8) [Caenorhabditis elegans] sp|P52713|MMSA_CAEEL Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial precursor (MMSDH) (Malonate-semialdehyde dehydrogenase [acylating]) 0.0
blast CAE59703 Hypothetical protein CBG03134 [Caenorhabditis briggsae] 0.0
blast EAA07972 ENSANGP00000022164 [Anopheles gambiae str. PEST] ref|XP_312441.2| ENSANGP00000022164 [Anopheles gambiae str. PEST] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002086 EMBL-EBI Aldehyde dehydrogenase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193502_S_AT
ggaagccggataccgacatcggaccactgatctccaagcaatccaaggcccgtgtcctcc
gtcttatcgagagtgccaagaaggagggagctcaggttccacttgatggatccaatatca
ctgttccaggatttgagaatggaaacttcgtgggaccaacgattctcgctggagtcaagc
caaatatgacttgctacagagaagaaatcttcggcccagtccttgtcgtcatggaggccg
agaacttgaacgaggccatcgaaatcatcaacaacaacccatacggaaacggaacggcca
tcttcaccagcaacggagccaccgccagaaagttcaccaacgaagtcgatgtcggacaaa
tcggaatcaacgtgccaatcccagttccactcccaatgttctcgttcaccggctcgcgtg
gttcattcctcggagatctcaacttctacggaaaggccggaatccagttctacactcaat
ggaagactgtcactcaatactggaacgagtcgctcaccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAGCCGGATACCGACATCGGACC 708 529 1028 Antisense
ACCACTGATCTCCAAGCAATCCAAG 669 85 1050 Antisense
TGTCCTCCGTCTTATCGAGAGTGCC 444 557 1080 Antisense
GAAGGAGGGAGCTCAGGTTCCACTT 542 331 1107 Antisense
GACCAACGATTCTCGCTGGAGTCAA 233 385 1181 Antisense
GAGAAGAAATCTTCGGCCCAGTCCT 334 389 1226 Antisense
TACGGAAACGGAACGGCCATCTTCA 457 645 1309 Antisense
TCGGAATCAACGTGCCAATCCCAGT 562 603 1388 Antisense
GTGGTTCATTCCTCGGAGATCTCAA 496 489 1445 Antisense
GAAAGGCCGGAATCCAGTTCTACAC 588 357 1478 Antisense
AATACTGGAACGAGTCGCTCACCGA 623 159 1523 Antisense
Sequence Source Affymetrix Proprietary Database