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[193500_s_at] N-oligosaccharyl transferase 48kd subunit

Description T09A5.11 /REP_DB=WormBase Gene ID /WP=CE01081 /TR=SW:P45971 /GB=CAA85337.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=N-oligosaccharyl transferase 48kd subunit
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 07      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 47      6grp_total > 08      4grp_total > 58      6grp_highest_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3492
1 2 3 4 range
A 2914.2
(-1.2)
P 		4452.9
(1.6)
P 		5242.5
(3)
P 		1907.2
(-3)
P 		3335
B 3207.6
(0.1)
P 		4613.2
(3)
P 		4603.8
(3)
P 		1751
(-3)
P 		2862
C 2868.9
(-0.8)
P 		4366.7
(2.9)
P 		4424.7
(3)
P 		1942.7
(-3)
P 		2482
avr 2996.9
(-0.7) 		4477.6
(2.4) 		4757
(3) 		1867
(-3) 		2890

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1985
1 2 3 4 5 6 range
A 4451.6
(3)
P 		2824.4
(-3)
P 		3035.3
(-2.2)
P 		2858
(-2.9)
P 		3319.9
(-1.2)
P 		2941.7
(-2.6)
P 		1627
B 4210.1
(3)
P 		3241.8
(-2.4)
P 		3134.1
(-3)
P 		3322.4
(-2)
P 		3150.9
(-2.9)
P 		3199.2
(-2.6)
P 		1076
C 3112.3
(-0.3)
P 		3398.6
(0.9)
P 		3237.8
(0.2)
P 		3898
(3)
P 		2467.1
(-3)
P 		3536.3
(1.5)
P 		1431
avr 3924.7
(3) 		3154.9
(-1.9) 		3135.7
(-2) 		3359.5
(-0.6) 		2979.3
(-3) 		3225.7
(-1.4) 		945
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1263
1 2 3 4 range
A 1061.1
(1.3)
P 		966.6
(0.8)
P 		1370.5
(3)
P 		279.2
(-3)
P 		1091
B 1022.9
(2.1)
P 		1139.4
(3)
P 		1066.8
(2.4)
P 		373.1
(-3)
P 		766
C 949.7
(0.1)
P 		1255.5
(1.6)
P 		1542.1
(3)
P 		327.6
(-3)
P 		1215
avr 1011.2
(1.1) 		1120.5
(1.8) 		1326.5
(3) 		326.6
(-3) 		1000

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 804
1 2 3 4 5 6 range
A 820.6
(-0.2)
P 		1004.5
(3)
P 		661
(-3)
P 		724.9
(-1.9)
P 		1001.8
(3)
P 		716.2
(-2)
P 		344
B 1464.9
(3)
P 		1079.1
(-0.3)
P 		761.1
(-3)
P 		880
(-2)
P 		1280.2
(1.4)
P 		1193
(0.7)
P 		704
C 1153.6
(2.5)
P 		1037
(0.8)
P 		824.1
(-2.4)
P 		783.9
(-3)
P 		798.3
(-2.8)
P 		1183.8
(3)
P 		400
avr 1146.4
(3) 		1040.2
(1.4) 		748.7
(-3) 		796.3
(-2.3) 		1026.8
(1.2) 		1031
(1.3) 		398
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193500_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4992
Sequence Type Exemplar sequence
Representative Public ID T09A5.11 NCBI
Target Description T09A5.11 /REP_DB=WormBase Gene ID /WP=CE01081 /TR=SW:P45971 /GB=CAA85337.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=N-oligosaccharyl transferase 48kd subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063254(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063254 NCBI Caenorhabditis elegans T09A5.11 (T09A5.11) mRNA, complete cds. 11/11 None
SNAP00000029442 ENSEMBL cdna:SNAP chromosome:CEL140:II:7864429:7865928:1 11/11 None
GENEFINDER00000029456 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7864429:7865928:1 11/11 None
T09A5.11.3 ENSEMBL cdna:known chromosome:CEL140:II:7863995:7866139:1 gene:T09A5.11 11/11 None
T09A5.11.2 ENSEMBL cdna:known chromosome:CEL140:II:7864426:7866143:1 gene:T09A5.11 11/11 None
T09A5.11.1 ENSEMBL cdna:known chromosome:CEL140:II:7864428:7866176:1 gene:T09A5.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7864425-7865925(+) 100.0 100.0
Public Domain and Genome References
Gene Title N-oligosaccharyl transferase 48kd subunit
Ensembl T09A5.11
Entrez Gene 174268 Entrez gene
SwissProt P45971 EMBL-EBI
WormBase CE01081 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247058_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit family protein at
CANINE:1602898_X_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase cfa
CANINE:1602897_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase cfa
CANINE:1582429_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase cfa
CANINE_2:CFA.3884.1.S1_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase cfa
CANINE_2:CFAAFFX.23148.1.S1_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase cfa
CANINE_2:CFAAFFX.23148.1.S1_S_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase cfa
CANINE:1605864_AT Oligosaccharyltransferase 48 kDa subunit cfa
DROSOPHILA_2:1625055_AT Oligosaccharyltransferase 48kD subunit dm
DROSGENOME1:142669_AT Oligosaccharyltransferase 48kD subunit dm
CHICKEN:GGA.13565.1.S1_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase gga
CHICKEN:GGAAFFX.6329.1.S1_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase gga
HG-U133_PLUS_2:208674_X_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-U133A_2:208674_X_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-U133A:208674_X_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-U133_PLUS_2:208675_S_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-U133A_2:208675_S_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-U133A:208675_S_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-FOCUS:208675_S_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HU35KSUBA:HUM_ALU_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HU35KSUBB:HUM_ALU_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HU35KSUBC:HUM_ALU_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HU35KSUBD:HUM_ALU_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HUGENEFL:HUM_ALU_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HC-G110:HUM_ALU_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HU35KSUBC:RC_H96850_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HUGENEFL:D29643_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
U133_X3P:G473936_3P_A_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
U133_X3P:208674_3P_X_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-U95AV2:38791_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-U95C:49030_S_AT dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
HG-U95D:87217_AT Dolichyl-diphosphooligosaccharide-protein glycosyltransferase hs
MOE430A:1416493_AT dolichyl-di-phosphooligosaccharide-protein glycotransferase mm
MOUSE430_2:1416493_AT dolichyl-di-phosphooligosaccharide-protein glycotransferase mm
MOUSE430A_2:1416493_AT dolichyl-di-phosphooligosaccharide-protein glycotransferase mm
MU11KSUBA:D89063_S_AT dolichyl-di-phosphooligosaccharide-protein glycotransferase mm
MG-U74AV2:101074_AT dolichyl-di-phosphooligosaccharide-protein glycotransferase mm
MU19KSUBB:TC33422_AT Dolichyl-di-phosphooligosaccharide-protein glycotransferase (Ddost), mRNA mm
RAE230A:1372247_AT dolichyl-di-phosphooligosaccharide-protein glycotransferase (predicted) rn
RAT230_2:1372247_AT dolichyl-di-phosphooligosaccharide-protein glycotransferase (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
18279 protein amino acid N-linked glycosylation via asparagine inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5789 endoplasmic reticulum membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85337 Hypothetical protein T09A5.11 [Caenorhabditis elegans] ref|NP_495655.1| T09A5.11 [Caenorhabditis elegans] sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor (Oligosaccharyl transferase 48 kDa subunit) 0.0
blast CAE59830 Hypothetical protein CBG03302 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005013 EMBL-EBI Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495655.1 1 404-426
T09A5.11.3 1 404-426
T09A5.11.2 1 404-426
T09A5.11.1 1 404-426
SNAP00000029442 1 404-426
GENEFINDER00000029456 1 404-426
Sequence
Target Sequence 
>C. ELEGANS:193500_S_AT
tccgcaaaaaccaactccgtcaatccttccgtacaaggagctcaatctggaaacgccgat
tttgctacagccatcactagatgggtgatgaaggagagtggagttcttcgtgtcaagact
gtcaatcaccacaagaaaggtgaaactgtaccaccagttgagggttatttcattaccgaa
gatgttgtttacaccattgaaatcgaagagttgaagaatggaaaatgggttccattccaa
ggaaaggacgttcaacttgagtttgttcgcatcgatccattcgtcagagcaacattgaag
aacagcaatggtcgtcttagcgttgctttcaagcttccagatgtgctcggagttttcaaa
ttcctggttgattacagacgcgttggatacactcatctctatgatgtgcaacaggtgtcg
gttcgtccattgtggcacacccagtatgaacgtttcattcgctccgcatatccatactac
gcctcttcgttctcaatgatggcgggactcgtgttgttctccatcgtctatctttatcat
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGCAAAAACCAACTCCGTCAATC 468 595 754 Antisense
AAGGAGCTCAATCTGGAAACGCCGA 170 159 788 Antisense
TTCGCATCGATCCATTCGTCAGAGC 21 697 1019 Antisense
CAATGGTCGTCTTAGCGTTGCTTTC 520 183 1059 Antisense
CAAGCTTCCAGATGTGCTCGGAGTT 539 185 1083 Antisense
TACAGACGCGTTGGATACACTCATC 629 643 1126 Antisense
GATGTGCAACAGGTGTCGGTTCGTC 132 409 1156 Antisense
TGGCACACCCAGTATGAACGTTTCA 590 545 1186 Antisense
ACGCCTCTTCGTTCTCAATGATGGC 456 91 1232 Antisense
AATGATGGCGGGACTCGTGTTGTTC 12 165 1248 Antisense
TTCTCCATCGTCTATCTTTATCATA 193 693 1270 Antisense
Sequence Source Affymetrix Proprietary Database