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[193497_at] guanine nucleotide-binding protein

Description F56H9.4 /REP_DB=WormBase Gene ID /WP=CE20886 /GEN=gpa-9 /TR=Q20910 /GB=CAA98953.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 0      6grp_highest_nonorm > 90      6grp_total > 09      4grp_total > 13      6grp_cgc > 10      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 113.8
(0.3)
P 		76.8
(-1.3)
A 		35.3
(-3)
A 		179.8
(3)
P 		145
B 87.4
(0.5)
P 		52.8
(-2)
A 		39.9
(-3)
A 		120.5
(3)
P 		81
C 104.6
(-0.1)
P 		35.7
(-3)
A 		37.4
(-2.9)
A 		176.4
(3)
P 		141
avr 101.9
(0.2) 		55.1
(-2.1) 		37.5
(-3) 		158.9
(3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 201.9
(3)
P 		126.9
(-1.2)
P 		198.1
(2.8)
P 		174.8
(1.5)
P 		138.9
(-0.5)
P 		94.9
(-3)
P 		107
B 211.8
(3)
P 		119.4
(-0.8)
P 		145.1
(0.2)
P 		133
(-0.3)
P 		131.8
(-0.3)
P 		67.5
(-3)
P 		144
C 258.3
(3)
P 		144.1
(-1.1)
P 		112.7
(-2.2)
P 		211.5
(1.3)
P 		105.6
(-2.4)
P 		90
(-3)
P 		168
avr 224
(3) 		130.1
(-1) 		152
(-0.1) 		173.1
(0.8) 		125.4
(-1.2) 		84.1
(-3) 		140
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 41.4
(3)
P 		16.7
(-1.6)
A 		9.2
(-3)
A 		26.3
(0.2)
P 		32
B 27.9
(3)
P 		13
(-1.8)
A 		9.2
(-3)
A 		25.7
(2.3)
P 		19
C 34.6
(3)
P 		10.3
(-3)
A 		13
(-2.3)
A 		29.7
(1.8)
P 		24
avr 34.6
(3) 		13.3
(-2.3) 		10.5
(-3) 		27.2
(1.2) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 37.2
(0.8)
P 		45.1
(3)
P 		43.2
(2.5)
P 		44.3
(2.8)
P 		41.9
(2.1)
P 		23.1
(-3)
P 		22
B 73.7
(3)
P 		39.7
(-1.2)
P 		35.2
(-1.8)
P 		35.2
(-1.8)
P 		53.5
(0.5)
P 		25.2
(-3)
P 		49
C 95.7
(3)
P 		44
(-1.6)
P 		28.7
(-3)
P 		42.5
(-1.8)
P 		34.2
(-2.5)
P 		30.1
(-2.9)
P 		67
avr 68.9
(3) 		42.9
(-0.6) 		35.7
(-1.7) 		40.7
(-1) 		43.2
(-0.6) 		26.1
(-3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193497_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13725
Sequence Type Exemplar sequence
Representative Public ID F56H9.4 NCBI
Target Description F56H9.4 /REP_DB=WormBase Gene ID /WP=CE20886 /GEN=gpa-9 /TR=Q20910 /GB=CAA98953.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073760(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073760 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-9) (gpa-9) mRNA, complete cds. 11/11 None
F56H9.4 ENSEMBL cdna:known chromosome:CEL140:V:12649429:12651903:-1 gene:F56H9.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036166 2/11 Cross Hyb Matching Probes None
GENEFINDER00000036172 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12649428-12651903(-) 99.06 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-9
Ensembl F56H9.4
Entrez Gene 191659 Entrez gene
SwissProt Q20910 EMBL-EBI
WormBase CE20886 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98953 Hypothetical protein F56H9.4 [Caenorhabditis elegans] ref|NP_506161.1| G Protein, Alpha subunit family member (gpa-9) [Caenorhabditis elegans] sp|Q20910|GPA9_CAEEL Guanine nucleotide-binding protein alpha-9 subunit 0.0
blast AAW02907 gpa-8 [Caenorhabditis briggsae] sp|Q61MC6|GPA8_CAEBR Guanine nucleotide-binding protein alpha-8 subunit emb|CAE63958.1| Hypothetical protein CBG08544 [Caenorhabditis briggsae] 1.0E-107
blast CAA98948 Hypothetical protein F56H9.3 [Caenorhabditis elegans] ref|NP_506160.1| G Protein, Alpha subunit family member (gpa-8) [Caenorhabditis elegans] gb|AAG32084.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q20907|GPA8_CAEEL Guanine nucleotide-binding protein alpha-8 subunit 1.0E-105
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 3.4E-15
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 2.0E-65
Sequence
Target Sequence 
>C. ELEGANS:193497_AT
tgaaaaccagagtcccaaccttgggaattgcacaatatgaagcaaatataaaacatttga
ctttcaaattcatttttttcgacattgggggacaacgtgctcaacgtcgaaaaattaccc
atctgagcggtcatgtgttagacgattcccatgcaattctttttattacttcactatcag
aatataatcaagttttgagagaagatgcttttgtgagccgaatagaagaatcacttcatt
tgtttaatgaaatatgcaatgggatgtattttcattcaaccgcaattattctatttttga
acaaaattgatcttttcgaaatcaaaatcactcacaccaacattacagtagcatttccag
actacgaaggtcctcgagaacgtgattgtgcacttgagtacattcgagttcaatttattt
ctctcaataataacaaaaatcggaaaatatatcagcatgtcacaagtgccacggatacag
ctagaattca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAAACCAGAGTCCCAACCTTGGG 432 569 632 Antisense
GACATTGGGGGACAACGTGCTCAAC 685 369 712 Antisense
ATTACCCATCTGAGCGGTCATGTGT 255 13 745 Antisense
TAGACGATTCCCATGCAATTCTTTT 603 651 770 Antisense
GGATGTATTTTCATTCAACCGCAAT 673 513 893 Antisense
AAAATCACTCACACCAACATTACAG 2 135 955 Antisense
GCATTTCCAGACTACGAAGGTCCTC 647 307 982 Antisense
GGTCCTCGAGAACGTGATTGTGCAC 708 501 1000 Antisense
GTGCACTTGAGTACATTCGAGTTCA 427 481 1019 Antisense
ATCAGCATGTCACAAGTGCCACGGA 454 29 1082 Antisense
GTGCCACGGATACAGCTAGAATTCA 534 477 1097 Antisense
Sequence Source Affymetrix Proprietary Database