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[193493_at] guanine nucleotide-binding protein

Description F56H9.3 /REP_DB=WormBase Gene ID /WP=CE05973 /GEN=gpa-8 /TR=Q20907 /GB=CAA98948.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_total > 18      4grp_total > 04      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 201
1 2 3 4 range
A 28.5
(-2)
P 		32.8
(-1.8)
A 		3.8
(-3)
A 		152.8
(3)
P 		149
B 62.9
(-1.2)
P 		35.3
(-2.3)
A 		18.3
(-3)
A 		163.5
(3)
P 		145
C 59.5
(-1.3)
P 		2.7
(-3)
A 		30
(-2.2)
A 		203.8
(3)
P 		201
avr 50.3
(-1.7) 		23.6
(-2.8) 		17.4
(-3) 		173.4
(3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 91
(3)
P 		36.7
(-1.7)
P 		47.5
(-0.8)
A 		36.5
(-1.8)
A 		29.3
(-2.4)
A 		22.2
(-3)
A 		69
B 60.3
(3)
P 		39.6
(0.6)
P 		12.8
(-2.5)
A 		8.7
(-3)
A 		25.2
(-1.1)
P 		21.3
(-1.5)
A 		52
C 58
(3)
P 		42.6
(1.2)
P 		7.9
(-3)
A 		40
(0.8)
A 		25.5
(-0.9)
P 		31
(-0.2)
P 		50
avr 69.8
(3) 		39.6
(-0.8) 		22.7
(-3) 		28.4
(-2.3) 		26.7
(-2.5) 		24.8
(-2.7) 		47
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 10.4
(-0.4)
P 		7.1
(-1.3)
A 		1
(-3)
A 		22.4
(3)
P 		21
B 20.1
(0.1)
P 		8.7
(-2.1)
A 		4.2
(-3)
A 		34.8
(3)
P 		31
C 19.7
(0.4)
P 		0.8
(-3)
A 		10.5
(-1.3)
A 		34.4
(3)
P 		34
avr 16.7
(-0.3) 		5.5
(-2.9) 		5.2
(-3) 		30.5
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 16.8
(3)
P 		13
(1)
P 		10.4
(-0.4)
A 		9.2
(-1)
A 		8.8
(-1.2)
A 		5.4
(-3)
A 		11
B 21
(3)
P 		13.2
(0.5)
P 		3.1
(-2.7)
A 		2.3
(-3)
A 		10.2
(-0.5)
P 		8
(-1.2)
A 		19
C 21.5
(3)
P 		13
(0.4)
P 		2
(-3)
A 		8
(-1.2)
A 		8.2
(-1.1)
P 		10.4
(-0.4)
P 		20
avr 19.8
(3) 		13.1
(0.2) 		5.2
(-3) 		6.5
(-2.5) 		9.1
(-1.4) 		7.9
(-1.9) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193493_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13616
Sequence Type Exemplar sequence
Representative Public ID F56H9.3 NCBI
Target Description F56H9.3 /REP_DB=WormBase Gene ID /WP=CE05973 /GEN=gpa-8 /TR=Q20907 /GB=CAA98948.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073759(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073759 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-8) (gpa-8) mRNA, complete cds. 11/11 None
SNAP00000036165 ENSEMBL cdna:SNAP chromosome:CEL140:V:12641441:12643059:-1 11/11 None
GENEFINDER00000036171 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12641441:12643059:-1 11/11 None
F56H9.3 ENSEMBL cdna:known chromosome:CEL140:V:12641441:12643059:-1 gene:F56H9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12641440-12643059(-) 98.08 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-8
Ensembl F56H9.3
Entrez Gene 179731 Entrez gene
SwissProt Q20907 EMBL-EBI
WormBase CE05973 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98948 Hypothetical protein F56H9.3 [Caenorhabditis elegans] ref|NP_506160.1| G Protein, Alpha subunit family member (gpa-8) [Caenorhabditis elegans] gb|AAG32084.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q20907|GPA8_CAEEL Guanine nucleotide-binding protein alpha-8 subunit 0.0
blast AAW02907 gpa-8 [Caenorhabditis briggsae] sp|Q61MC6|GPA8_CAEBR Guanine nucleotide-binding protein alpha-8 subunit emb|CAE63958.1| Hypothetical protein CBG08544 [Caenorhabditis briggsae] 1.0E-180
blast CAA98953 Hypothetical protein F56H9.4 [Caenorhabditis elegans] ref|NP_506161.1| G Protein, Alpha subunit family member (gpa-9) [Caenorhabditis elegans] sp|Q20910|GPA9_CAEEL Guanine nucleotide-binding protein alpha-9 subunit 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 7.9E-19
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 3.5E-106
Sequence
Target Sequence 
>C. ELEGANS:193493_AT
ggactatttaccaaccgaagaagatattctgaaaagcagagttccaacatctggagttat
tcaatacaaaattatgctgaaaaattttaattttagaatatttgacgtcggaggtcaacg
agcccagcgaagaaaatggcttcatgtatttgatgatgttcaagctgttctttttataac
ttcattatcagaatatgatcaagttttaagagaagatgctactgtgaatcgaatgaaaga
atcgttgaatttatttgagaaaatatgtaatggaaggtattttctcaacactgcaatgat
attatttttaaataaaattgatcttttcaaaattaaaatcaagcataccaatattacagt
tgcattgacgagttataaaggtccacaggaatgtgatagcgcacttgactacattcgaaa
gcgattcatttcattgaataaaaacaaaaaacgctcaatttatgaacatgtgacttgtgc
cacagacactgaacagattcaggtggttattgattcggtcattgatgttgtgattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACTATTTACCAACCGAAGAAGAT 19 525 540 Antisense
GAAAAGCAGAGTTCCAACATCTGGA 106 355 570 Antisense
AGGTCAACGAGCCCAGCGAAGAAAA 404 45 651 Antisense
CAAGCTGTTCTTTTTATAACTTCAT 516 185 700 Antisense
GAAGGTATTTTCTCAACACTGCAAT 262 335 812 Antisense
ATTACAGTTGCATTGACGAGTTATA 607 11 892 Antisense
GGTCCACAGGAATGTGATAGCGCAC 707 501 919 Antisense
GATAGCGCACTTGACTACATTCGAA 111 425 934 Antisense
GACTTGTGCCACAGACACTGAACAG 462 371 1011 Antisense
GATTCAGGTGGTTATTGATTCGGTC 705 429 1035 Antisense
GATTCGGTCATTGATGTTGTGATTC 262 433 1051 Antisense
Sequence Source Affymetrix Proprietary Database