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[193486_at] zinc finger DNA-binding protein

Description F16H11.5 /REP_DB=WormBase Gene ID /WP=CE04400 /GEN=nhr-45 /TR=Q19496 /GB=AAA98005.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=zinc finger DNA-binding protein
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 26      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 50      6grp_total > 25      4grp_total > 22      6grp_highest_sub > De      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 574
1 2 3 4 range
A 850.7
(1.4)
P 		534.8
(-2.2)
P 		461.6
(-3)
P 		989.8
(3)
P 		528
B 1035.7
(3)
P 		573.7
(-2.4)
P 		526.9
(-3)
P 		868
(1)
P 		509
C 934.4
(3)
P 		591.5
(-2.2)
P 		539.2
(-3)
P 		835.7
(1.5)
P 		395
avr 940.3
(3) 		566.7
(-2.2) 		509.2
(-3) 		897.8
(2.4) 		431

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 478
1 2 3 4 5 6 range
A 692
(2.2)
P 		754.8
(3)
P 		356.9
(-2.2)
P 		372
(-2)
P 		296.3
(-3)
P 		339.7
(-2.4)
P 		459
B 521.9
(3)
P 		519.7
(2.9)
P 		450.9
(0.9)
P 		318.5
(-3)
P 		325.3
(-2.8)
P 		320.9
(-2.9)
P 		203
C 728.1
(3)
P 		512
(0.1)
P 		453.4
(-0.6)
P 		314
(-2.5)
P 		276.5
(-3)
P 		418.1
(-1.1)
P 		452
avr 647.3
(3) 		595.5
(2.1) 		420.4
(-0.9) 		334.8
(-2.4) 		299.4
(-3) 		359.6
(-2) 		348
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 309.7
(3)
P 		116.1
(-3)
P 		120.7
(-2.9)
P 		144.9
(-2.1)
P 		194
B 330.3
(3)
P 		141.7
(-2.4)
P 		122.1
(-3)
P 		185
(-1.2)
P 		208
C 309.3
(3)
P 		170.1
(-2)
P 		187.9
(-1.3)
P 		140.9
(-3)
P 		168
avr 316.4
(3) 		142.6
(-3) 		143.6
(-3) 		156.9
(-2.5) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 207
1 2 3 4 5 6 range
A 127.6
(-1.4)
P 		268.4
(3)
P 		77.7
(-3)
P 		94.3
(-2.5)
P 		89.4
(-2.6)
P 		82.7
(-2.8)
P 		191
B 181.6
(3)
P 		173
(2.5)
P 		109.5
(-1.5)
P 		84.4
(-3)
P 		132.2
(-0)
P 		119.7
(-0.8)
P 		97
C 269.9
(3)
P 		156.2
(-0.3)
P 		115.4
(-1.5)
P 		63.2
(-3)
P 		89.5
(-2.2)
P 		140
(-0.8)
P 		207
avr 193
(2.7) 		199.2
(3) 		100.9
(-2) 		80.6
(-3) 		103.7
(-1.8) 		114.1
(-1.3) 		119
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193486_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16457
Sequence Type Exemplar sequence
Representative Public ID F16H11.5 NCBI
Target Description F16H11.5 /REP_DB=WormBase Gene ID /WP=CE04400 /GEN=nhr-45 /TR=Q19496 /GB=AAA98005.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=zinc finger DNA-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076396(11), AF273786(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076396 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-45) (nhr-45) mRNA, complete cds. 11/11 None
AF273786 NCBI Caenorhabditis elegans clone yk347g3 nuclear receptor NHR-45 (nhr-45) pseudogene, mRNA sequence. 11/11 A 
GENEFINDER00000010353 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4655454:4660269:-1 11/11 None
SNAP00000010348 ENSEMBL cdna:SNAP chromosome:CEL140:X:4656849:4659026:-1 11/11 None
F16H11.5 ENSEMBL cdna:known chromosome:CEL140:X:4656404:4660332:-1 gene:F16H11.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4656796-4660333(-) 100.0 100.0
Public Domain and Genome References
Gene Title zinc finger DNA-binding protein
Gene Symbol nhr-45
Ensembl F16H11.5
Entrez Gene 180738 Entrez gene
SwissProt Q19496 EMBL-EBI
WormBase CE33052 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
3707 steroid hormone receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA98005 Nuclear hormone receptor family protein 45 [Caenorhabditis elegans] ref|NP_508797.2| Nuclear Hormone Receptor family member (nhr-45) [Caenorhabditis elegans] 0.0
blast CAE68628 Hypothetical protein CBG14515 [Caenorhabditis briggsae] 0.0
blast CAE68628 Hypothetical protein CBG14515 [Caenorhabditis briggsae] 1.0E-174
blast CAB03299 Hypothetical protein T06C12.7 [Caenorhabditis elegans] ref|NP_506968.1| Nuclear Hormone Receptor family member (nhr-84) [Caenorhabditis elegans] 3.0E-14
blast CAB03299 Hypothetical protein T06C12.7 [Caenorhabditis elegans] ref|NP_506968.1| Nuclear Hormone Receptor family member (nhr-84) [Caenorhabditis elegans] 5.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 4.8E-9
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 2.0E-17
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 4.8E-9
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 2.3E-11
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 4.8E-9
Sequence
Target Sequence 
>C. ELEGANS:193486_AT
gtatgtctattcgagttttcgcccaacttccaaaggatcagaagtggattatcttcaagc
acttttggacgaggttctatgaattagatagatgttttgcaacttgccaacgactcggct
ataacctcactgacgagagggggctcacgctgaatggtcagattatcaattttggaatat
ctgttgtgaaactagagaatattagtgatatggatgctacacaagttagaaatttcctaa
aaggaagtatggataagttccgattgatctttatcaacccgttcaaaaagttgcaaccaa
ccgagtatgagctcatgtacatgatgatgagcataatgtggagtgtatcaaacctccctg
gaatcactgatgctactagggatatatctgaaaaggttgagcttcggttagctgaagatc
tccatacttattatgctgaacagtatgacaacaacaatccaaactacgctggtagaataa
cacggctaagtagcattgcctccgctgttgatgagatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATGTCTATTCGAGTTTTCGCCCA 615 455 1040 Antisense
TTTTCGCCCAACTTCCAAAGGATCA 87 675 1055 Antisense
CAAGCACTTTTGGACGAGGTTCTAT 111 185 1095 Antisense
TGCAACTTGCCAACGACTCGGCTAT 585 575 1137 Antisense
TCGGCTATAACCTCACTGACGAGAG 319 607 1154 Antisense
TGACGAGAGGGGGCTCACGCTGAAT 121 571 1170 Antisense
GTTGCAACCAACCGAGTATGAGCTC 277 437 1329 Antisense
AAACCTCCCTGGAATCACTGATGCT 350 125 1389 Antisense
TCGGTTAGCTGAAGATCTCCATACT 136 605 1443 Antisense
ACGGCTAAGTAGCATTGCCTCCGCT 526 93 1521 Antisense
CATTGCCTCCGCTGTTGATGAGATC 473 217 1533 Antisense
Sequence Source Affymetrix Proprietary Database