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[193482_at] guanine nucleotide-binding protein

Description T07A9.7 /REP_DB=WormBase Gene ID /WP=CE17216 /GEN=gpa-4 /TR=O44409 /GB=AAK39279.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_total > 14      4grp_total > 23      6grp_cgc > 30      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 179.2
(2.7)
P
87.6
(-2.9)
A
85.8
(-3)
A
184.6
(3)
A
99
B 134.3
(3)
P
56.9
(-3)
A
110.9
(1.2)
A
116.9
(1.7)
A
77
C 162.6
(3)
P
87.1
(-0.5)
P
33.6
(-3)
A
115.3
(0.8)
A
129
avr 158.7
(3)
77.2
(-3)
76.8
(-3)
138.9
(1.5)
82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 118.5
(3)
A
101.6
(1.8)
A
90.7
(1)
P
93.7
(1.2)
A
55.1
(-1.7)
A
37.1
(-3)
A
81
B 137.4
(3)
P
88
(0.1)
A
74.8
(-0.7)
A
61.6
(-1.5)
A
36.5
(-3)
A
42
(-2.7)
A
101
C 144.2
(3)
P
105.6
(0.5)
A
63.5
(-2.2)
A
98.1
(0.1)
A
50.2
(-3)
A
62.9
(-2.2)
A
94
avr 133.4
(3)
98.4
(0.6)
76.3
(-1)
84.5
(-0.4)
47.3
(-3)
47.3
(-3)
86
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 65.2
(3)
P
19
(-3)
A
22.4
(-2.6)
A
27
(-2)
A
46
B 42.8
(3)
P
14
(-3)
A
25.7
(-0.6)
A
24.9
(-0.7)
A
29
C 53.8
(3)
P
25
(-1.1)
P
11.7
(-3)
A
19.4
(-1.9)
A
42
avr 53.9
(3)
19.3
(-3)
19.9
(-2.9)
23.8
(-2.2)
35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 21.8
(-0.2)
A
36.2
(3)
A
19.7
(-0.6)
P
23.8
(0.3)
A
16.6
(-1.3)
A
9
(-3)
A
27
B 47.8
(3)
P
29.3
(-0.4)
A
18.2
(-2.4)
A
16.3
(-2.7)
A
14.8
(-3)
A
15.7
(-2.8)
A
33
C 53.4
(3)
P
32.2
(-0.4)
A
16.2
(-3)
A
19.7
(-2.4)
A
16.2
(-3)
A
21.1
(-2.2)
A
37
avr 41
(3)
32.6
(1)
18
(-2.4)
19.9
(-1.9)
15.9
(-2.9)
15.3
(-3)
26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193482_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11166
Sequence Type Exemplar sequence
Representative Public ID T07A9.7 NCBI
Target Description T07A9.7 /REP_DB=WormBase Gene ID /WP=CE17216 /GEN=gpa-4 /TR=O44409 /GB=AAK39279.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067520(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_067520 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-4) (gpa-4) mRNA, complete cds. 11/11 None
SNAP00000017764 ENSEMBL cdna:SNAP chromosome:CEL140:IV:402932:404352:1 10/11 None
GENEFINDER00000017780 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:401734:404836:1 11/11 None
T07A9.7 ENSEMBL cdna:known chromosome:CEL140:IV:401695:405480:1 gene:T07A9.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017763 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:401733-404352(+) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-4
Ensembl T07A9.7
Entrez Gene 176865 Entrez gene
SwissProt Q9BIG5 EMBL-EBI
WormBase CE31599 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK39279 G protein, alpha subunit protein 4 [Caenorhabditis elegans] ref|NP_499921.2| G Protein, Alpha subunit family member (gpa-4) [Caenorhabditis elegans] gb|AAG32080.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q9BIG5|GPA4_CAEEL Guanine nucleotide-binding protein alpha-4 subunit 0.0
blast AAW02903 gpa-4 [Caenorhabditis briggsae] sp|Q61B55|GPA4_CAEBR Guanine nucleotide-binding protein alpha-4 subunit emb|CAE67895.1| Hypothetical protein CBG13491 [Caenorhabditis briggsae] 1.0E-160
blast AAW02903 gpa-4 [Caenorhabditis briggsae] sp|Q61B55|GPA4_CAEBR Guanine nucleotide-binding protein alpha-4 subunit emb|CAE67895.1| Hypothetical protein CBG13491 [Caenorhabditis briggsae] 1.0E-140
blast CAA78807 GTP-binding protein alpha-i subunit [Lymnaea stagnalis] sp|P30682|GNAI_LYMST Guanine nucleotide-binding protein G(i), alpha subunit (Adenylate cyclase-inhibiting G alpha protein) 1.0E-128
blast CAA78807 GTP-binding protein alpha-i subunit [Lymnaea stagnalis] sp|P30682|GNAI_LYMST Guanine nucleotide-binding protein G(i), alpha subunit (Adenylate cyclase-inhibiting G alpha protein) 1.0E-109
blast AAK39279 G protein, alpha subunit protein 4 [Caenorhabditis elegans] ref|NP_499921.2| G Protein, Alpha subunit family member (gpa-4) [Caenorhabditis elegans] gb|AAG32080.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q9BIG5|GPA4_CAEEL Guanine nucleotide-binding protein alpha-4 subunit 1.0E-105
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.3E-110
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Sequence
Target Sequence
>C. ELEGANS:193482_AT
ttctcactggagctctctgatttaatgaagcaattgtggatggatgaaggtgtcaagaaa
tgcgttaaacgagcacatgaatatcagctgaacgactcggctgagtattacttcaacgca
ttggatcgtatctcatcatcctcttacttgccaactcaggatgatattcttagagctcgt
gtcaagtcaactggcattgttgagacaaccttcatgtataaggatttgtgtttcaaaatg
ttcgacgtcggaggtcaacgttcggaacgtaagaagtggatacattgctttgatagtgta
actgcagtcatattctgtgttgcactcagcgagtatgacttgagactcgccgaagaccag
actatgaaccgaatgcacgaatcaatgcaattgtttgactcaatcgtgaacaattgctgg
tttaccgagacatcgattatcctattccttaacaaaatggacattttcgaggaaagaatc
cgttacacaccattgaccgtctgtttcccagagtatcaa


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
TTCTCACTGGAGCTCTCTGATTTAA 643 691 376 Antisense
GACTCGGCTGAGTATTACTTCAACG 275 375 469 Antisense
TGGATCGTATCTCATCATCCTCTTA 149 551 497 Antisense
CATCCTCTTACTTGCCAACTCAGGA 629 211 512 Antisense
TAGAGCTCGTGTCAAGTCAACTGGC 675 651 546 Antisense
GCAGTCATATTCTGTGTTGCACTCA 63 313 679 Antisense
GACTTGAGACTCGCCGAAGACCAGA 212 369 712 Antisense
GACCAGACTATGAACCGAATGCACG 19 385 730 Antisense
GAGACATCGATTATCCTATTCCTTA 475 391 802 Antisense
TACACACCATTGACCGTCTGTTTCC 157 643 859 Antisense
GACCGTCTGTTTCCCAGAGTATCAA 616 381 870 Antisense
Sequence Source Affymetrix Proprietary Database