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[193481_at]

Description H08M01.2 /REP_DB=WormBase Gene ID /WP=CE16171 /TR=O45611 /GB=CAB07634.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=GTPase-activator protein for Rho-like GTPases
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 23      4grp_highest_8_8 > 63      4grp_high_8_8 > 19      6grp_highest_nonorm > 61      6grp_total > 03      4grp_total > 29      6grp_highest_sub > De      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 277
1 2 3 4 range
A 512.7
(3)
P 		413.8
(-0.1)
P 		318.9
(-3)
P 		447.8
(1)
P 		194
B 413.5
(3)
P 		354.6
(-0)
P 		297.4
(-3)
P 		394.1
(2)
P 		116
C 384.3
(0.7)
P 		388.7
(0.8)
P 		235.8
(-3)
P 		477.2
(3)
P 		241
avr 436.8
(2.9) 		385.7
(0.9) 		284
(-3) 		439.7
(3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 337
1 2 3 4 5 6 range
A 532.5
(2.6)
P 		551.3
(3)
P 		477
(1.3)
P 		388.3
(-0.6)
P 		513.8
(2.2)
P 		281.8
(-3)
P 		270
B 564.6
(3)
P 		547.7
(2.6)
P 		432.1
(0.2)
P 		552.7
(2.7)
P 		446
(0.5)
P 		281.4
(-3)
P 		283
C 413
(-1)
P 		618.2
(3)
P 		440.9
(-0.5)
P 		494.4
(0.6)
P 		407
(-1.2)
P 		313.1
(-3)
P 		305
avr 503.4
(1.5) 		572.4
(3) 		450
(0.4) 		478.5
(1) 		455.6
(0.5) 		292.1
(-3) 		280
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 186.7
(3)
P 		89.8
(-1.8)
P 		83.4
(-2.1)
P 		65.6
(-3)
P 		121
B 131.9
(3)
P 		87.6
(-1.2)
P 		68.9
(-3)
P 		84
(-1.6)
P 		63
C 127.2
(3)
P 		111.8
(1)
P 		82.2
(-2.8)
P 		80.5
(-3)
P 		47
avr 148.6
(3) 		96.4
(-1.4) 		78.2
(-2.9) 		76.7
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 98.2
(-1.6)
P 		196.1
(3)
P 		103.9
(-1.3)
P 		98.5
(-1.6)
P 		155.1
(1.1)
P 		68.6
(-3)
P 		128
B 196.4
(3)
P 		182.3
(2.1)
P 		104.9
(-3)
P 		146.4
(-0.3)
P 		181.2
(2)
P 		104.9
(-3)
P 		92
C 153.1
(0.6)
P 		188.6
(3)
P 		112.2
(-2.1)
P 		99.4
(-3)
P 		131.7
(-0.8)
P 		104.8
(-2.6)
P 		89
avr 149.2
(0.5) 		189
(3) 		107
(-2.1) 		114.8
(-1.6) 		156
(0.9) 		92.8
(-3) 		96
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193481_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10141
Sequence Type Exemplar sequence
Representative Public ID H08M01.2 NCBI
Target Description H08M01.2 /REP_DB=WormBase Gene ID /WP=CE16171 /TR=O45611 /GB=CAB07634.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=GTPase-activator protein for Rho-like GTPases
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028086(10), NM_001028085(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028086 NCBI Caenorhabditis elegans H08M01.2b (H08M01.2) mRNA, complete cds. 10/11 None
NM_001028085 NCBI Caenorhabditis elegans H08M01.2a (H08M01.2) mRNA, complete cds. 11/11 None
GENEFINDER00000031795 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13836966:13844878:1 10/11 None
H08M01.2b ENSEMBL cdna:known chromosome:CEL140:IV:13827855:13844947:1 gene:H08M01.2 10/11 None
H08M01.2a ENSEMBL cdna:known chromosome:CEL140:IV:13827870:13844681:1 gene:H08M01.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000031788 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13829981-13844816(+) 100.0 100.0
Public Domain and Genome References
Ensembl H08M01.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07634 Hypothetical protein H08M01.2b [Caenorhabditis elegans] ref|NP_001023257.1| H08M01.2b [Caenorhabditis elegans] 0.0
blast CAE54901 Hypothetical protein H08M01.2a [Caenorhabditis elegans] ref|NP_001023256.1| H08M01.2a [Caenorhabditis elegans] 0.0
blast CAE74601 Hypothetical protein CBG22384 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000198 EMBL-EBI RhoGAP domain 1.2E-51
Pfam IPR000198 EMBL-EBI RhoGAP domain 9.3E-52
Pfam IPR000198 EMBL-EBI RhoGAP domain 1.2E-51
Sequence
Target Sequence 
>C. ELEGANS:193481_AT
gatctaagcacatcccttatcaactaccctgcaagtacgagtacacaaggagtcattgct
gcaaatgaaacattggagacattatgctcaaaatctccttcccaaataccaatttatctt
gagaaatgtatacaatttattgaacagaatggaggttttgaacaagagggactgtaccga
gtacctggaaatcaaactcatttggcagaagttgagaaacggtttttgaaatatggagaa
ttcgatgtctcctcgtttgacacgcctgtccacgtggcagcaaccgccctgaagagcttt
ttctcgtgcctaccggaatcattaataccgacggcttaccatttacggtggaaacaaatt
atgatggtttcagatgatattaagaaaatcgacggaatccgtgatgcgttggcaattctt
ccggtttcaaatcaaaaagtccttcaatatctagtcactcacctcacaaaagtgtcatgt
tctccaaaaaccgtaatgaattcaaataatttgtcaaaagtgtggacgcccacactgttt
aggtatgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTAAGCACATCCCTTATCAACT 621 419 2764 Antisense
CCTTATCAACTACCCTGCAAGTACG 260 265 2778 Antisense
ACATTATGCTCAAAATCTCCTTCCC 584 105 2842 Antisense
GAGGGACTGTACCGAGTACCTGGAA 256 401 2929 Antisense
AGAATTCGATGTCTCCTCGTTTGAC 694 75 3000 Antisense
TTCTCGTGCCTACCGGAATCATTAA 519 687 3064 Antisense
TACCGACGGCTTACCATTTACGGTG 131 649 3089 Antisense
GACGGAATCCGTGATGCGTTGGCAA 671 373 3154 Antisense
TTGGCAATTCTTCCGGTTTCAAATC 205 707 3172 Antisense
GTCCTTCAATATCTAGTCACTCACC 147 477 3202 Antisense
GACGCCCACACTGTTTAGGTATGTA 476 379 3288 Antisense
Sequence Source Affymetrix Proprietary Database